Ensemble Prostate Tumor Classification in H&E Whole Slide Imaging via Stain Normalization and Cell Density Estimation

  • Michaela Weingant
  • Hayley M. Reynolds
  • Annette Haworth
  • Catherine Mitchell
  • Scott Williams
  • Matthew D. DiFrancoEmail author
Conference paper
Part of the Lecture Notes in Computer Science book series (LNCS, volume 9352)


Classification of prostate tumor regions in digital histology images requires comparable features across datasets. Here we introduce adaptive cell density estimation and apply H&E stain normalization into a supervised classification framework to improve inter-cohort classifier robustness. The framework uses Random Forest feature selection, class-balanced training example subsampling and support vector machine (SVM) classification to predict the presence of high- and low-grade prostate cancer (HG-PCa and LG-PCa) on image tiles. Using annotated whole-slide prostate digital pathology images to train and test on two separate patient cohorts, classification performance, as measured with area under the ROC curve (AUC), was 0.703 for HG-PCa and 0.705 for LG-PCa. These results improve upon previous work and demonstrate the effectiveness of cell-density and stain normalization on classification of prostate digital slides across cohorts.


Machine learning Digital pathology Tumor prediction Prostate Cell counting 


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© Springer International Publishing Switzerland 2015

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Authors and Affiliations

  • Michaela Weingant
    • 1
  • Hayley M. Reynolds
    • 2
    • 3
  • Annette Haworth
    • 2
    • 3
  • Catherine Mitchell
    • 4
  • Scott Williams
    • 5
  • Matthew D. DiFranco
    • 6
    Email author
  1. 1.Vienna University of TechnologyViennaAustria
  2. 2.Department of Physical SciencesPeter MacCallum Cancer CentreMelbourneAustralia
  3. 3.Sir Peter MacCallum Department of OncologyUniversity of MelbourneMelbourneAustralia
  4. 4.Department of PathologyPeter MacCallum Cancer CentreMelbourneAustralia
  5. 5.Division of Radiation Oncology and Cancer ImagingPeter MacCallum Cancer CentreMelbourneAustralia
  6. 6.Center for Medical Physics and Biomedical EngineeringMedical University ViennaViennaAustria

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