Mapping the Protein–Protein Interactome Networks Using Yeast Two-Hybrid Screens

  • Seesandra Venkatappa RajagopalaEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 883)


The yeast two-hybrid system (Y2H) is a powerful method to identify binary protein–protein interactions in vivo. Here we describe Y2H screening strategies that use defined libraries of open reading frames (ORFs) and cDNA libraries. The array-based Y2H system is well suited for interactome studies of small genomes with an existing ORFeome clones preferentially in a recombination based cloning system. For large genomes, pooled library screening followed by Y2H pairwise retests may be more efficient in terms of time and resources, but multiple sampling is necessary to ensure comprehensive screening. While the Y2H false positives can be efficiently reduced by using built-in controls, retesting, and evaluation of background activation; implementing the multiple variants of the Y2H vector systems is essential to reduce the false negatives and ensure comprehensive coverage of an interactome.


Yeast two-hybrid system Protein–protein interactions Pooled library screening Two-hybrid array 



Work on this chapter was funded by NIH grant RO1GM79710.


  1. Bader JS, Chaudhuri A, Rothberg JM, Chant J (2004) Gaining confidence in high-throughput protein interaction networks. Nat Biotechnol 22(1):78–85CrossRefPubMedGoogle Scholar
  2. Bennett ST, Barnes C, Cox A, Davies L, Brown C (2005) Toward the 1,000 dollars human genome. Pharmacogenomics 6(4):373–382. doi: 10.1517/14622416.6.4.373 CrossRefPubMedGoogle Scholar
  3. Braun P, Tasan M, Dreze M, Barrios-Rodiles M, Lemmens I, Yu H, Sahalie JM, Murray RR, Roncari L, de Smet AS, Venkatesan K, Rual JF, Vandenhaute J, Cusick ME, Pawson T, Hill DE, Tavernier J, Wrana JL, Roth FP, Vidal M (2009) An experimentally derived confidence score for binary protein-protein interactions. Nat Methods 6(1):91–97PubMedCentralCrossRefPubMedGoogle Scholar
  4. Brent R, Ptashne M (1985) A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell 43(3 Pt 2):729–736CrossRefPubMedGoogle Scholar
  5. Cagney G, Uetz P, Fields S (2001) Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome. Physiol Genomics 7(1):27–34PubMedGoogle Scholar
  6. Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff E, Johannsen E (2007) Epstein-Barr virus and virus human protein interaction maps. Proc Natl Acad Sci U S A 104(18):7606–7611. doi: 10.1073/pnas.0702332104 PubMedCentralCrossRefPubMedGoogle Scholar
  7. Chen YC, Rajagopala SV, Stellberger T, Uetz P (2010) Exhaustive benchmarking of the yeast two-hybrid system. Nat Methods 7(9):667–668, author reply 8CrossRefPubMedGoogle Scholar
  8. de Chassey B, Navratil V, Tafforeau L, Hiet MS, Aublin-Gex A, Agaugue S, Meiffren G, Pradezynski F, Faria BF, Chantier T, Le Breton M, Pellet J, Davoust N, Mangeot PE, Chaboud A, Penin F, Jacob Y, Vidalain PO, Vidal M, Andre P, Rabourdin-Combe C, Lotteau V (2008) Hepatitis C virus infection protein network. Mol Syst Biol 4:230. doi: 10.1038/msb.2008.66 PubMedCentralCrossRefPubMedGoogle Scholar
  9. Ester C, Uetz P (2008) The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71. BMC Biochem 9:29. doi: 10.1186/1471-2091-9-29 PubMedCentralCrossRefPubMedGoogle Scholar
  10. Estojak J, Brent R, Golemis EA (1995) Correlation of two-hybrid affinity data with in vitro measurements. Mol Cell Biol 15(10):5820–5829PubMedCentralCrossRefPubMedGoogle Scholar
  11. Fields S, Song O (1989) A novel genetic system to detect protein-protein interactions. Nature 340(6230):245–246CrossRefPubMedGoogle Scholar
  12. Gavin AC, Bosche M, Krause R, Grandi P, Marzioch M, Bauer A, Schultz J, Rick JM, Michon AM, Cruciat CM, Remor M, Hofert C, Schelder M, Brajenovic M, Ruffner H, Merino A, Klein K, Hudak M, Dickson D, Rudi T, Gnau V, Bauch A, Bastuck S, Huhse B, Leutwein C, Heurtier MA, Copley RR, Edelmann A, Querfurth E, Rybin V, Drewes G, Raida M, Bouwmeester T, Bork P, Seraphin B, Kuster B, Neubauer G, Superti-Furga G (2002) Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415(6868):141–147CrossRefPubMedGoogle Scholar
  13. Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL Jr, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM (2003) A protein interaction map of Drosophila melanogaster. Science 302(5651):1727–1736CrossRefPubMedGoogle Scholar
  14. Goll J, Uetz P (2006) The elusive yeast interactome. Genome Biol 7(6):223PubMedCentralCrossRefPubMedGoogle Scholar
  15. Gong W, Shen YP, Ma LG, Pan Y, Du YL, Wang DH, Yang JY, Hu LD, Liu XF, Dong CX, Ma L, Chen YH, Yang XY, Gao Y, Zhu D, Tan X, Mu JY, Zhang DB, Liu YL, Dinesh-Kumar SP, Li Y, Wang XP, Gu HY, Qu LJ, Bai SN, Lu YT, Li JY, Zhao JD, Zuo J, Huang H, Deng XW, Zhu YX (2004) Genome-wide ORFeome cloning and analysis of Arabidopsis transcription factor genes. Plant Physiol 135(2):773–782PubMedCentralCrossRefPubMedGoogle Scholar
  16. Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ (1993) The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75(4):805–816CrossRefPubMedGoogle Scholar
  17. Hauser R, Ceol A, Rajagopala SV, Mosca R, Siszler G, Wermke N, Sikorski P, Schwarz F, Schick M, Wuchty S, Aloy P, Uetz P (2014) A second-generation protein-protein interaction network of Helicobacter pylori. Mol Cell Proteomics 13(5):1318–1329. doi: 10.1074/mcp.O113.033571 PubMedCentralCrossRefPubMedGoogle Scholar
  18. Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, Luhrmann R, Stelzl U (2012) Dynamic protein-protein interaction wiring of the human spliceosome. Mol Cell 45(4):567–580. doi: 10.1016/j.molcel.2011.12.034 CrossRefPubMedGoogle Scholar
  19. Ho Y, Gruhler A, Heilbut A, Bader GD, Moore L, Adams SL, Millar A, Taylor P, Bennett K, Boutilier K, Yang L, Wolting C, Donaldson I, Schandorff S, Shewnarane J, Vo M, Taggart J, Goudreault M, Muskat B, Alfarano C, Dewar D, Lin Z, Michalickova K, Willems AR, Sassi H, Nielsen PA, Rasmussen KJ, Andersen JR, Johansen LE, Hansen LH, Jespersen H, Podtelejnikov A, Nielsen E, Crawford J, Poulsen V, Sorensen BD, Matthiesen J, Hendrickson RC, Gleeson F, Pawson T, Moran MF, Durocher D, Mann M, Hogue CW, Figeys D, Tyers M (2002) Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature 415(6868):180–183CrossRefPubMedGoogle Scholar
  20. Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y (2001) A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A 98(8):4569–4574PubMedCentralCrossRefPubMedGoogle Scholar
  21. James P, Halladay J, Craig EA (1996) Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144(4):1425–1436PubMedCentralPubMedGoogle Scholar
  22. Jin F, Avramova L, Huang J, Hazbun T (2007) A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nat Methods 4(5):405–407PubMedCentralPubMedGoogle Scholar
  23. Joung JK, Ramm EI, Pabo CO (2000) A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97(13):7382–7387PubMedCentralCrossRefPubMedGoogle Scholar
  24. Kerrien S, Aranda B, Breuza L, Bridge A, Broackes-Carter F, Chen C, Duesbury M, Dumousseau M, Feuermann M, Hinz U, Jandrasits C, Jimenez RC, Khadake J, Mahadevan U, Masson P, Pedruzzi I, Pfeiffenberger E, Porras P, Raghunath A, Roechert B, Orchard S, Hermjakob H (2012) The IntAct molecular interaction database in 2012. Nucleic Acids Res 40(Database issue):D841–D846. doi:  10.1093/nar/gkr1088
  25. Khadka S, Vangeloff AD, Zhang C, Siddavatam P, Heaton NS, Wang L, Sengupta R, Sahasrabudhe S, Randall G, Gribskov M, Kuhn RJ, Perera R, LaCount DJ (2011) A physical interaction network of dengue virus and human proteins. Mol Cell Proteomics 10(12):M111 012187. doi:  10.1074/mcp.M111.012187
  26. Koegl M, Uetz P (2007) Improving yeast two-hybrid screening systems. Brief Funct Genomic Proteomic 6(4):302–312CrossRefPubMedGoogle Scholar
  27. Lacomble S, Portman N, Gull K (2009) A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod. PLoS One 4(11), e7685. doi: 10.1371/journal.pone.0007685 PubMedCentralCrossRefPubMedGoogle Scholar
  28. Lamesch P, Milstein S, Hao T, Rosenberg J, Li N, Sequerra R, Bosak S, Doucette-Stamm L, Vandenhaute J, Hill DE, Vidal M (2004) C. elegans ORFeome version 3.1: increasing the coverage of ORFeome resources with improved gene predictions. Genome Res 14(10B):2064–2069PubMedCentralCrossRefPubMedGoogle Scholar
  29. Landy A (1989) Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem 58:913–949CrossRefPubMedGoogle Scholar
  30. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Alenquer ML, Jarvie TP, Jirage KB, Kim JB, Knight JR, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, McKenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu P, Begley RF, Rothberg JM (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437(7057):376–380. doi: 10.1038/nature03959 PubMedCentralPubMedGoogle Scholar
  31. Memisevic V, Zavaljevski N, Pieper R, Rajagopala SV, Kwon K, Townsend K, Yu C, Yu X, DeShazer D, Reifman J, Wallqvist A (2013) Novel Burkholderia mallei virulence factors linked to specific host-pathogen protein interactions. Mol Cell Proteomics 12(11):3036–3051. doi: 10.1074/mcp.M113.029041 PubMedCentralCrossRefPubMedGoogle Scholar
  32. Parrish JR, Yu J, Liu G, Hines JA, Chan JE, Mangiola BA, Zhang H, Pacifico S, Fotouhi F, DiRita VJ, Ideker T, Andrews P, Finley RL Jr (2007) A proteome-wide protein interaction map for Campylobacter jejuni. Genome Biol 8(7):R130PubMedCentralCrossRefPubMedGoogle Scholar
  33. Rain JC, Selig L, De Reuse H, Battaglia V, Reverdy C, Simon S, Lenzen G, Petel F, Wojcik J, Schachter V, Chemama Y, Labigne A, Legrain P (2001) The protein-protein interaction map of Helicobacter pylori. Nature 409(6817):211–215CrossRefPubMedGoogle Scholar
  34. Rajagopala SV, Titz B, Goll J, Parrish JR, Wohlbold K, McKevitt MT, Palzkill T, Mori H, Finley RL Jr, Uetz P (2007) The protein network of bacterial motility. Mol Syst Biol 3:128PubMedCentralCrossRefPubMedGoogle Scholar
  35. Rajagopala SV, Hughes KT, Uetz P (2009) Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins. Proteomics 9(23):5296–5302PubMedCentralCrossRefPubMedGoogle Scholar
  36. Rajagopala SV, Yamamoto N, Zweifel AE, Nakamichi T, Huang HK, Mendez-Rios JD, Franca-Koh J, Boorgula MP, Fujita K, Suzuki K, Hu JC, Wanner BL, Mori H, Uetz P (2010) The Escherichia coli K-12 ORFeome: a resource for comparative molecular microbiology. BMC Genomics 11:470PubMedCentralCrossRefPubMedGoogle Scholar
  37. Rajagopala SV, Casjens S, Uetz P (2011) The protein interaction map of bacteriophage lambda. BMC Microbiol 11:213. doi: 10.1186/1471-2180-11-213 PubMedCentralCrossRefPubMedGoogle Scholar
  38. Rajagopala SV, Sikorski P, Caufield JH, Tovchigrechko A, Uetz P (2012) Studying protein complexes by the yeast two-hybrid system. Methods 58(4):392–399. doi: 10.1016/j.ymeth.2012.07.015 PubMedCentralCrossRefPubMedGoogle Scholar
  39. Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014) The binary protein-protein interaction landscape of Escherichia coli. Nat Biotechnol 32(3):285–290. doi: 10.1038/nbt.2831 PubMedCentralCrossRefPubMedGoogle Scholar
  40. Raquet X, Eckert JH, Muller S, Johnsson N (2001) Detection of altered protein conformations in living cells. J Mol Biol 305(4):927–938CrossRefPubMedGoogle Scholar
  41. Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las RJ, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP, Vidal M (2014) A proteome-scale map of the human interactome network. Cell 159(5):1212–1226. doi: 10.1016/j.cell.2014.10.050 PubMedCentralCrossRefPubMedGoogle Scholar
  42. Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, Hill DE, Vidal M (2004) Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res 14(10B):2128–2135PubMedCentralCrossRefPubMedGoogle Scholar
  43. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature 437(7062):1173–1178CrossRefPubMedGoogle Scholar
  44. Schwartz H, Alvares CP, White MB, Fields S (1998) Mutation detection by a two-hybrid assay. Hum Mol Genet 7(6):1029–1032CrossRefPubMedGoogle Scholar
  45. SenGupta DJ, Zhang B, Kraemer B, Pochart P, Fields S, Wickens M (1996) A three-hybrid system to detect RNA-protein interactions in vivo. Proc Natl Acad Sci U S A 93(16):8496–8501PubMedCentralCrossRefPubMedGoogle Scholar
  46. Shapira SD, Gat-Viks I, Shum BO, Dricot A, de Grace MM, Wu L, Gupta PB, Hao T, Silver SJ, Root DE, Hill DE, Regev A, Hacohen N (2009) A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection. Cell 139(7):1255–1267. doi: 10.1016/j.cell.2009.12.018 PubMedCentralCrossRefPubMedGoogle Scholar
  47. Stanyon CA, Limjindaporn T, Finley RL Jr (2003) Simultaneous cloning of open reading frames into several different expression vectors. Biotechniques 35(3):520–522, 4–6PubMedGoogle Scholar
  48. Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P (2010) Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome. Proteome Sci 8:8PubMedCentralCrossRefPubMedGoogle Scholar
  49. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksoz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell 122(6):957–968CrossRefPubMedGoogle Scholar
  50. Titz B, Rajagopala SV, Goll J, Hauser R, McKevitt MT, Palzkill T, Uetz P (2008) The binary protein interactome of Treponema pallidum--the syphilis spirochete. PLoS One 3(5):e2292. doi:  10.1371/journal.pone.0002292
  51. Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, Johnston M, Fields S, Rothberg JM (2000) A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 403(6770): 623–627CrossRefPubMedGoogle Scholar
  52. Uetz P, Dong YA, Zeretzke C, Atzler C, Baiker A, Berger B, Rajagopala SV, Roupelieva M, Rose D, Fossum E, Haas J (2006) Herpesviral protein networks and their interaction with the human proteome. Science 311(5758):239–242CrossRefPubMedGoogle Scholar
  53. Vidal M, Endoh H (1999) Prospects for drug screening using the reverse two-hybrid system. Trends Biotechnol 17(9):374–381CrossRefPubMedGoogle Scholar
  54. Vidal M, Legrain P (1999) Yeast forward and reverse ‘n’-hybrid systems. Nucleic Acids Res 27(4):919–929PubMedCentralCrossRefPubMedGoogle Scholar
  55. von Brunn A, Teepe C, Simpson JC, Pepperkok R, Friedel CC, Zimmer R, Roberts R, Baric R, Haas J (2007) Analysis of intraviral protein-protein interactions of the SARS coronavirus ORFeome. PLoS One 2(5), e459. doi: 10.1371/journal.pone.0000459 CrossRefGoogle Scholar
  56. von Mering C, Jensen LJ, Kuhn M, Chaffron S, Doerks T, Kruger B, Snel B, Bork P (2007) STRING 7--recent developments in the integration and prediction of protein interactions. Nucleic Acids Res 35(Database issue):D358–D362CrossRefGoogle Scholar
  57. Walhout AJ, Temple GF, Brasch MA, Hartley JL, Lorson MA, van den Heuvel S, Vidal M (2000) GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol 328:575–592CrossRefPubMedGoogle Scholar
  58. Wang X, Wei X, Thijssen B, Das J, Lipkin SM, Yu H (2012) Three-dimensional reconstruction of protein networks provides insight into human genetic disease. Nat Biotechnol 30(2):159–164. doi: 10.1038/nbt.2106 PubMedCentralCrossRefPubMedGoogle Scholar
  59. Yu H, Braun P, Yildirim MA, Lemmens I, Venkatesan K, Sahalie J, Hirozane-Kishikawa T, Gebreab F, Li N, Simonis N, Hao T, Rual JF, Dricot A, Vazquez A, Murray RR, Simon C, Tardivo L, Tam S, Svrzikapa N, Fan C, de Smet AS, Motyl A, Hudson ME, Park J, Xin X, Cusick ME, Moore T, Boone C, Snyder M, Roth FP, Barabasi AL, Tavernier J, Hill DE, Vidal M (2008) High-quality binary protein interaction map of the yeast interactome network. Science (New York, NY) 322(5898):104–110. doi:  10.1126/science.1158684
  60. Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M (2011) Next-generation sequencing to generate interactome datasets. Nat Methods 8(6):478–480. doi: 10.1038/nmeth.1597 PubMedCentralCrossRefPubMedGoogle Scholar

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© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.J. Craig Venter InstituteRockvilleUSA

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