Theoretical Aspects

  • Alexander P. Demchenko
Chapter

Abstract

Quantitative measures are highly needed in fluorescence sensing. In this Chapter the reader finds discussion on parameters that have to be optimized in every sensor, such as selectivity, sensitivity and limit of detection. Concentrating on reversible binding, in which the mass action law is observed, the methods of determining the binding constants and modeling the ligand binding isotherms are presented. Kinetics of target binding and its influence on the results of analyte determination are discussed. Focusing on fluorescence sensing, linear and intensity-weighted formats are distinguished and analyzed. At the end of this Chapter the reader finds the Section “Sensing and thinking” with the list of questions and problems. It addresses the issue, how to provide the optimal quantitative measure of target binding.

Keywords

Sensor sensitivity and limit of detection Reversible/irreversible binding Mass action law Binding constants Binding isotherms Binding kinetics Fluorescence Linear/weighted response 
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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Alexander P. Demchenko
    • 1
  1. 1.Laboratory of Nanobiotechnology Palladin Institute of BiochemistryNational Academy of Sciences of UkraineKievUkraine

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