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Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines

  • Marko Popovic
  • Kaspar Vogt
  • Knut Holthoff
  • Arthur Konnerth
  • Brian M. Salzberg
  • Amiram Grinvald
  • Srdjan D. Antic
  • Marco Canepari
  • Dejan Zecevic
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 859)

Abstract

A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on how exactly signals are integrated by individual nerve cells functioning as complex operational units. Regional electrical properties of branching neuronal processes which determine the input-output function of any neuron are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor, at multiple sites, subthreshold events as they travel from the sites of origin (synaptic contacts on distal dendrites) and summate at particular locations to influence action potential initiation. It became possible recently to carry out this type of measurement using high-resolution multisite recording of membrane potential changes with intracellular voltage-sensitive dyes. This chapter reviews the development and foundation of the method of voltage-sensitive dye recording from individual neurons. Presently, this approach allows monitoring membrane potential transients from all parts of the dendritic tree as well as from axon collaterals and individual dendritic spines.

Keywords

Fluorescence Glutamate uncaging Voltage-imaging Potentiometric probes Voltage-sensitive dyes Neurons Axons Dendrites Dendritic spines 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Marko Popovic
    • 1
  • Kaspar Vogt
    • 2
  • Knut Holthoff
    • 3
  • Arthur Konnerth
    • 4
  • Brian M. Salzberg
    • 5
    • 6
  • Amiram Grinvald
    • 7
  • Srdjan D. Antic
    • 8
  • Marco Canepari
    • 9
  • Dejan Zecevic
    • 1
  1. 1.Department of Cellular and Molecular PhysiologyYale University School of MedicineNew HavenUSA
  2. 2.Division of Pharmacology and NeurobiologyBiozentrum – University of BaselBaselSwitzerland
  3. 3.Hans Berger Klinik für NeurologieFriedrich-Schiller-Universität JenaJenaGermany
  4. 4.Center for Integrated Protein Science and Institute of NeuroscienceTechnical University MunichMunichGermany
  5. 5.Department of NeuroscienceUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  6. 6.Department of PhysiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  7. 7.Department of NeurobiologyWeizmann Institute of ScienceRehovotIsrael
  8. 8.Department of NeuroscienceU. Conn. Health CenterFarmingtonUSA
  9. 9.Laboratoire Interdisciplinare de Physique(CNRS Université Joseph Fourier UMR 5588)GrenobleFrance

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