Chymotrypsin Probe for Real-Time Visualization of Pancreatic Leak

  • Suguru Yamashita
  • Takeaki Ishizawa
  • Masayo Sakabe
  • Mako Kamiya
  • Yasuteru UranoEmail author
  • Norihiro KokudoEmail author


Pancreatic fistula remains the most serious complication after digestive surgery. This is mainly because there are no techniques to identify pancreatic leak from the pancreatic stump during surgery. Nor have there been any techniques for rapid evaluation of protease activity in leaking fluid during surgery that can cause severe tissue damage. We have designed and developed a novel fluorescence probe (glutaryl phenylalanine hydroxymethyl rhodamine green) that is activated by chymotrypsin. The use of this novel probe is expected to result in a reduced incidence of pancreatic fistula and operative mortality after digestive surgery.


Pancreatic fistula Pancreatic leak Glutaryl phenylalanine hydroxymethyl rhodamine green Chymotrypsin 


Financial Disclosure: The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in this manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, and royalties. No writing assistance was used in the production of this manuscript.

Funding/Support: This work was supported by grants from the Takeda Science Foundation, the Ministry of Education, Culture, Sports, Science, Technology of Japan, and the Ministry of Health, Labour, and Welfare of Japan, and Global Leader Program for Social Design and Management.

Supplementary material

Video 36.1

Fluorescence imaging of leaking pancreatic juice using the chymotrypsin probe. Fluorescence imaging clearly visualized leakage of pancreatic juice along a stapler line beginning 3 min after spraying chymotrypsin probe on the pancreatic stump (WMV 39,304 kb)


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Suguru Yamashita
    • 1
  • Takeaki Ishizawa
    • 2
    • 3
  • Masayo Sakabe
    • 4
  • Mako Kamiya
    • 4
  • Yasuteru Urano
    • 4
    Email author
  • Norihiro Kokudo
    • 3
    Email author
  1. 1.Department of Surgery, Graduate School of MedicineUniversity of TokyoBunkyo-kuJapan
  2. 2.Department of Gastroenterological SurgeryCancer Institute Hospital, Japanese Foundation for Cancer ResearchKoto-kuJapan
  3. 3.Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of MedicineUniversity of TokyoBunkyo-kuJapan
  4. 4.Laboratory of Chemical Biology and Molecular Imaging, Graduate School of MedicineUniversity of TokyoBunkyo-kuJapan

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