Advertisement

Taurine 9 pp 167-177 | Cite as

Effect of Taurine on Viability and Proliferation of Murine Melanoma B16F10 Cells

  • Yon-Suk Kim
  • Sun Hee Cheong
  • Jin-Woo Hwang
  • Gaurav Lodhi
  • Kwang-Ho Lee
  • Dong-Kug Choi
  • Hyuk Song
  • Sang-Hoon Lee
  • Dong-June Park
  • Chang-Bum Ahn
  • Sang-Ho Moon
  • Byong-Tae Jeon
  • Pyo-Jam ParkEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 803)

Abstract

This study was aimed at investigating the possible mechanism of the anti-proliferative effect of taurine on B16F10 melanoma cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red assays and microscopic analysis. Apoptotic cells were detected by flow cytometry analysis. The proteins involved in apoptosis and cellular differentiation were assessed by immunoblot analysis. Taurine inhibited cell proliferation and induced cell death in B16F10 cells. In this study, taurine was used to examine whether it could be a potential chemotherapy agent for skin cancer. Our data suggest that taurine inhibits B16F10 cell proliferation by inducing apoptosis and cellular differentiation.

Keywords

Taurine B16F10 Anti-proliferation Apoptosis Cell viability Cell cycle Doxorubicin Flow cytometry Hoechst 33342 Skin cancer 

Notes

Acknowledgements

This study was supported by a grant from the Korea Food Research Institute (Project name: Development of silver foods for the elderly)

References

  1. Balkan J, Kanbagli O, Aykac-Toker G, Uysal M (2002) Taurine treatment reduces hepatic lipids and oxidative stress in chronically ethanol-treated rats. Biol Pharm Bull 25:1231–1233CrossRefPubMedGoogle Scholar
  2. Basu A, Haldar S (1998) The relationship between BcI2, Bax and p53: consequences for cell cycle progression and cell death. Mol Hum Reprod 4:1099–1109CrossRefPubMedGoogle Scholar
  3. Carmichael J, Degraff WG, Gamson J, Russo D, Gazdar AF, Levitt ML, Minna JD, Mitchell JB (1989) Radiation sensitivity of human lung cancer cell lines. Eur J Cancer Clin Oncol 25:527–534CrossRefPubMedGoogle Scholar
  4. D’Agostini C, Pica F, Febbraro G, Grelli S, Chiavaroli C, Garaci E (2005) Antitumour effect of OM-174 and Cyclophosphamide on murine B16 melanoma in different experimental conditions. Int Immunopharmacol 5:1205–1212CrossRefPubMedGoogle Scholar
  5. Choi HS, Cha YN, Kim CK (2006) Taurine chloramine inhibits PMA-stimulated superoxide production in human neutrophils perhaps by inhibiting phosphorylation and translocation of p47phox. Int Immunopharmacol 6:1431–1440CrossRefPubMedGoogle Scholar
  6. Condron C, Casey RG, Kehoe S, Toomey D, Creagh T, Bouchier-Hayes DJ (2010) Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder. Urol Res 38:215–222CrossRefPubMedGoogle Scholar
  7. Das J, Vasan V, Sil PC (2012) Taurine exerts hypoglycemic effect in alloxan induced diabetic rats, improves insulin-mediated glucose transport signaling pathway in heart and ameliorates cardiac oxidative stress and apoptosis. Toxicol Appl Pharmacol 258:296–308CrossRefPubMedGoogle Scholar
  8. Das J, Sil PC (2012) Taurine ameliorates alloxan-induced diabetic renal injury, oxidative stress-related signaling pathways and apoptosis in rats. Amino Acids 43:1509–1523CrossRefPubMedGoogle Scholar
  9. Gottardi W, Nagl M (2010) N-chlorotaurine, a natural antiseptic with outstanding tolerability. J Antimicrob Chemother 65:399–409CrossRefPubMedGoogle Scholar
  10. Henderson JP, Byun J, Williams MV, Mueller DM, McCormick ML, Heinecke JW (2001) Production of brominating intermediates by myeloperoxidase: a transhalogenation pathway for generating mutagenic nucleobases during inflammation. J Biol Chem 276:7867–7875CrossRefPubMedGoogle Scholar
  11. Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ (1993) Bcl-2 function in an antioxidant pathway to prevent apoptosis. Cell 75:241–251CrossRefPubMedGoogle Scholar
  12. Kerai MD, Waterfield CJ, Kenyon SH, Asker DS, Timbrell JA (1998) Taurine: protective properties against ethanol-induced hepatic steatosis and lipid peroxidation during chronic ethanol consumption in rats. Amino Acids 15:53–76CrossRefPubMedGoogle Scholar
  13. Marciani DJ, Press JB, Reynolds RC, Pathak AK, Pathak V, Gundy LE, Farmer JT, Koratich MS, May RD (2000) Development of semisynthetic triterpenoid saponin derivatives with immune stimulating activity. Vaccine 18:3141–3151CrossRefPubMedGoogle Scholar
  14. Marcinkiewicz J, Kontny E (2014) Taurine and inflammatory diseases. Amino Acids 46:7–20CrossRefPubMedCentralPubMedGoogle Scholar
  15. Matsuda M, Asano Y (2012) A simple assay of taurine concentrations in food and biological samples using taurine dioxygenase. Anal Biochem 427:121–123CrossRefPubMedGoogle Scholar
  16. Marcinkiewicz J (2009) Taurine bromamine: a new therapeutic option in inflammatory skin diseases. Pol Arch Med Wewn 119:673–675PubMedGoogle Scholar
  17. Nagl M, Hess MW, Pfaller K, Hengster P, Gottardi W (2000) Bactericidal activity of micromolar N-chlorotaurine: evidence for its antimicrobial function in the human defense system. Antimicrob Agents Chemother 44:2507–2513CrossRefPubMedCentralPubMedGoogle Scholar
  18. Parra A, Rivas F, Martin-Fonseca S, Garcia-Granados A, Martinez A (2011) Maslinic acid derivatives induce significant apoptosis in b16f10 murine melanoma cells. Eur J Med Chem 46:5991–6001CrossRefPubMedGoogle Scholar
  19. Ribeiro RA, Vanzela EC, Oliveira CAM, Bonfleur ML, Boschero AC, Carneiro EM (2010) Taurine supplementation: involvement of cholinergic/phospholipase C and protein kinase A pathways in potentiation of insulin secretion and Ca2+ handling in mouse pancreatic islets. Br J Nutr 104:1148–1155CrossRefPubMedGoogle Scholar
  20. Roy A, Sil PC (2012) Taurine protects murine hepatocytes against oxidative stress-induced apoptosis by tert-butyl hydroperoxide via PI3K/Akt and mitochondrial-dependent pathways. Food Chem 131:1086–1096CrossRefGoogle Scholar
  21. Shalby AB, Assaf N, Ahmed HH (2011) Possible mechanisms for N-acetyl cysteine and taurine in ameliorating acute renal failure induced by cisplatin in rats. Toxicol Mech Methods 21:538–546CrossRefPubMedGoogle Scholar
  22. Shao X, Hu ZT, Hu CY, Bu Q, Yan GY, Deng PC, Lv L, Wu D, Deng Y, Zhao JX, Zhu RM, Li Y, Li H, Xu Y, Yang H, Zhao Y, Cen X (2012) Taurine protects methamphetamine-induced developmental angiogenesis defect through antioxidant mechanism. Toxicol Appl Pharmacol 260:260–270CrossRefPubMedGoogle Scholar
  23. Shivananjappa MM, Muralidhara (2012) Taurine attenuates maternal and embryonic oxidative stress in a streptozotocin diabetic rat model. Reprod Biomed Online 24:558–566CrossRefPubMedGoogle Scholar
  24. Turna G, Kilic N, Yildirim Z, Sari S (2011) Effects of Thymus sipyleus and taurine on hepatic MDA, GSH, AOPP levels and SOD activity in Ehrlich Acide solid tumor model generated mice. Turk Klin Tip Bilim Derg 31:1153–1159Google Scholar
  25. Ueki I, Stipanuk MH (2009) 3T3-L1 adipocytes and rat adipose tissue have a high capacity for taurine synthesis by the cysteine dioxygenase/cysteinesulfinate decarboxylase and cysteamine dioxygenase pathways. J Nutr 139:207–214CrossRefPubMedCentralPubMedGoogle Scholar
  26. Weiss SJ, Klein R, Slivka A, Wei M (1982) Chlorination of taurine by human neutrophils: evidence for hypochlorous acid generation. J Clin Invest 70:598–607CrossRefPubMedCentralPubMedGoogle Scholar
  27. Yang L, Latchoumycandane C, McMullen MR, Pratt BT, Zhang R, Papouchado BG, Nagy LE, Feldstein AE, McIntyre TM (2010) Chronic alcohol exposure increases circulating bioactive oxidized phospholipids. J Biol Chem 285:22211–22220CrossRefPubMedCentralPubMedGoogle Scholar
  28. Yildirim Z, Kilic N (2011) Effects of taurine and age on cerebellum antioxidant status and oxidative stress. Int J Gerontol 5:166–170CrossRefGoogle Scholar
  29. Zhang J, Sun R, Wei H, Tian Z (2005) Antitumor effects of recombinant human prolactin in human adenocarcinoma-bearing SCID mice with human NK cell xenograft. Int Immunopharmacol 5:417–425CrossRefPubMedGoogle Scholar
  30. Zulli A (2011) Taurine in cardiovascular disease. Curr Opin Clin Nutr Metab Care 14:57–60CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Yon-Suk Kim
    • 1
  • Sun Hee Cheong
    • 1
    • 2
  • Jin-Woo Hwang
    • 1
  • Gaurav Lodhi
    • 1
  • Kwang-Ho Lee
    • 1
  • Dong-Kug Choi
    • 1
  • Hyuk Song
    • 3
  • Sang-Hoon Lee
    • 4
  • Dong-June Park
    • 5
  • Chang-Bum Ahn
    • 6
  • Sang-Ho Moon
    • 2
  • Byong-Tae Jeon
    • 2
  • Pyo-Jam Park
    • 1
    • 2
    Email author
  1. 1.Department of BiotechnologyKonkuk UniversityChungjuSouth Korea
  2. 2.Nokyong Research CenterKonkuk UniversityChungjuSouth Korea
  3. 3.Department of Food Bioscience, Research Institute for Biomedical & Health Science, College of Biomedical & Health ScienceKonkuk UniversityChungjuSouth Korea
  4. 4.Food Resource Research Center, Korea Food Research InstituteRepublic of Korea University of Science and TechnologyDaejeonSouth Korea
  5. 5.Food Resource Research CenterKorea Food Research InstituteSeongnamSouth Korea
  6. 6.Division of Food and NutritionChonnam National UniversityGwangjuSouth Korea

Personalised recommendations