Traditional Balsamic Vinegar as Coded by Us



There are several descriptions of how to make balsamic vinegars. Benedetti mentions 21 traditional methods that are still practised on a household level (Benedetti 2004); some of these are quite unusual, others are reasonable but very generic. Recently, a procedure was patented (Patent BO2011A000132) perfectly compatible with the product regulations for TBV, which clearly sets out all the steps for TBV production. Ideally, the process is divided into four main stages, with a separate description of the characteristics of the grapes. The stages are: cooking of grape must (GM), alcoholic fermentation of cooked must (CKM), acetic fermentation of base wine (BW), and maturation. The latter includes the chemical and physical transformations that occur during all stages, beginning with Maillard reactions generated by the heat treatment of the must, and ending with the same Maillard reactions in the barrel set. In the intermediate stages, two important microbiological transformations occur: alcoholic and acetic acid fermentations.


Titratable Acidity Maillard Reaction Gluconic Acid Alcoholic Fermentation Grape Variety 
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  1. Amerine MA, Kunkee RE (1968) Microbiology of winemaking. Annu Rev Microbiol 22:323–358CrossRefGoogle Scholar
  2. Asai T (1968) Oxidation of other aliphatic monoalcohols. In: Acetic acid bacteria: classification and biochemical activities. University of Tokyo Press, Japan, pp 141–151Google Scholar
  3. Benedetti B (2004) Fare in casa l’aceto balsamico, 2nd edn. Edizioni Il fiorino, ModenaGoogle Scholar
  4. Canas S, Belchior P, Mateus AM, Spranger MI, Bruno-de-Sousa R (2002) Kinetics of impregnation/evaporation and release of phenolic compounds from wood to brandy in experimental model. Ciência Téc Vitic 17:1–14Google Scholar
  5. Ciani M (1998) Wine vinegar production using base wines made with different yeast species. J Sci Food Agric 78:290–294CrossRefGoogle Scholar
  6. Ciani M, Maccarelli F (1998) Oenological properties of non-Saccharomyces yeasts associated with wine-making. Word J Microbiol Biotechnol 14:199–203CrossRefGoogle Scholar
  7. Davenport RR (1974) Microecology of yeast and yeast-like organisms associated with and English vineyard. Vitis 13:123–130Google Scholar
  8. Deak T, Beuchat LR (1996) Handbook of food spoilage yeasts. CRC, Boca Raton, FLGoogle Scholar
  9. De Vero l, Gala E, Gullo M, Solieri L, Landi S, Giudici P (2006) Application of denaturing gradient gel electrophoresis (DGGE) analysis to evaluate acetic acid bacteria in traditional balsamic vinegar. Food Mirobiology 23:809–813CrossRefGoogle Scholar
  10. Falcone PM, Giudici P (2010) Sugar conversion induced by the application of heat to the grape must. J Agric Food Sci 58:8680–8691CrossRefGoogle Scholar
  11. Fleet GH (1992) Spoilage yeast. Crit Rev Microbiol 12:1–44Google Scholar
  12. Fleet GH (2003) Yeast interactions and wine flavour. Int J Food Microbiol 86:11–22CrossRefGoogle Scholar
  13. Fleet GH, Heard GM (1993) Yeasts-growth during fermentation. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood, Chur, Switzerland, pp 27–55Google Scholar
  14. Gobbi M, De Vero L, Solieri L, Comitini F (2014) Fermentative aptitude of non-Saccharomyces wine yeast for reduction in the ethanol content in wine. European Food Research and Technology 239:41–48Google Scholar
  15. Giudici P (1990) Inibizione dell’acido acetico sull’attività dei lieviti osmofili isolati dall’aceto balsamico tradizionale. Industrie delle Bevande 110:475–480Google Scholar
  16. Giudici P (1993) Acido gluconico: criterio di genuinità nell’aceto balsamico tradizionale. Industrie delle Bevande 124:123–125Google Scholar
  17. Giudici P, Zambonelli C (1992) Criteri di selezione dei lieviti per enologia. Vignevini 9:29–34Google Scholar
  18. Giudici P, Altieri C, Cavalli R (1992) Aceto balsamico tradizionale, preparazione del fermentato di base. Industrie delle Bevande 122:478–483Google Scholar
  19. Giudici P, Solieri L, Pulvirenti AM, Cassanelli S (2005) Strategies and perspectives for genetic improvement of wine yeasts. Appl Microbiol Biotechnol 66:622–628CrossRefGoogle Scholar
  20. Granchi L, Ganucci D, Messini A, Vincenzini M (2002) Oenological properties of Hanseniaspora osmophila and Kloeckera corticis from wines produced by spontaneous fermentations of normal and dried grapes. FEMS Yeast Res 2:403–407Google Scholar
  21. Gullo M, Giudici P (2008) Acetic acid bacteria in traditional balsamic vinegar: phenotypic traits relevant for starter cultures selection. Int J Food Microbiol 125:46–53CrossRefGoogle Scholar
  22. Gullo M, Caggia C, De Vero L, Giudici P (2006) Characterization of acetic acid bacteria in traditional balsamic vinegar. Int J Food Microbiol 106:209–212CrossRefGoogle Scholar
  23. Gullo M, De Vero L, Giudici P (2009) Succession of selected strains of Acetobacter pasteurianus and other acetic acid bacteria in Traditional Balsamic Vinegar. Appl Environ Microbiol 75(8):2585–2589CrossRefGoogle Scholar
  24. Landi S, Castellari L, Gullo M, Solieri L, De Vero L, Giudici P (2005) Yeasts and sugar of traditional balsamic vinegar. In: Vinegars and acetic acid bacteria international symposium, Reggio Emilia, ItalyGoogle Scholar
  25. Lemmetti F, Giudici P (2010) Bilancio di massa ed età dell’Aceto Balsamico Tradizionale. Industrie delle Bevande 230:18–28Google Scholar
  26. Loureiro V, Malfeito-Ferreira M (2003) Spoilage yeasts in wine industries. Int J Food Microbiol 86:23–50CrossRefGoogle Scholar
  27. Muratore G, Licciardello F, Restuccia C, Puglisi ML, Giudici P (2006) Role of different factors affecting the formation of 5-hydroxymethyl-2-furancarboxaldehyde in heated grape must. J Agric Food Chem 54:860–863CrossRefGoogle Scholar
  28. Patent No BO2011A000132 (Italy) Procedimento per la produzione di un condimento agrodolce invecchiato di mosto d’uva cottoGoogle Scholar
  29. Pitt JI (1975) Xerophilic fungi and the spoilage of foods of plant origin. In: Duckworth RB (ed) Water relations of foods. Academic, London, pp 273–307CrossRefGoogle Scholar
  30. Sacchetti M (1974) Sull’aceto balsamico modenese. Edagricole, BolognaGoogle Scholar
  31. Saeki A (1990) Studies on acetic acid fermentation. III continuous production of vinegar with immobilized Saccharomycodes ludwigii cells and immobilized Acetobacter aceti cells entrapped in calcium alginate gel beads. J Jpn Soc Food Sci 37:722–725CrossRefGoogle Scholar
  32. Siau JE (1984) Transport process in wood. Springer, BerlinCrossRefGoogle Scholar
  33. Solieri L, Giudici P (2008) Yeasts associated to Traditional Balsamic Vinegar: ecological and technological features. Int J Food Microbiol 125(1):36–45CrossRefGoogle Scholar
  34. Solieri L, Gullo M, De Vero L, Antunez O, Perez-ortin JE, Giudici P (2005) Homogeneity of interspecific hybrids between Saccharomyces cerevisiae and Saccharomyces uvarum by phenotypic and transcriptional analysis. Int J Biotechnol Biochem 1:11–21Google Scholar
  35. Solieri L, Landi S, De Vero L, Giudici P (2006) Molecular assessment of indigenous yeast population from traditional balsamic vinegar. J Appl Microbiol 101(1):63–71CrossRefGoogle Scholar
  36. Solieri L, Cassanelli S, Giudici P (2007) A new putative Zygosaccharomyces yeast species isolated from traditional balsamic vinegar. Yeast 24:403–417CrossRefGoogle Scholar
  37. Turtura GC, Benfenati L (1988) Caratteristiche microbiologiche e chimiche dell’Aceto Balsamico Naturale. Studio del prodotto. Ann Microbiol 38:51–74Google Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Life SciencesUniversity of Modena and Reggio EmiliaReggio EmiliaItaly
  2. 2.ModenaItaly

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