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Structural Changes of GPI Anchor After Its Attachment to Proteins: Functional Significance

  • Taroh KinoshitaEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 842)

Abstract

One hundred and fifty or more of human proteins are anchored to the outer leaflet of plasma membrane by a glycolipid, termed glycosylphosphatidylinositol (GPI). While GPI-anchored proteins (GPI-APs) have wide range of functions, they share two unique characteristics due to the common membrane-anchors. One is that GPI-APs are mainly associated with membrane microdomains so called membrane rafts. Recent reports demonstrated that GPI-APs exist as homodimers and that homodimerization is important for their raft association. Another characteristic of GPI-APs is release from the cell surface after cleavage by GPI-cleaving enzymes. Preassembled GPI is transferred en bloc by GPI transamidase to the C-terminus of proteins having a C-terminal GPI-attachment signal peptide. A unique feature of GPI-anchor is that GPI structure is dynamically modified during transport to the cell surface. Structural remodeling of lipid and glycan moieties in the endoplasmic reticulum is critical for efficient recruitment of GPI-APs into ER-exit site and association with their cargo receptors. Fatty acid remodeling of GPI in the Golgi is important for homodimerization and raft association. These structure–function relationships will be discussed.

Keywords

Chinese Hamster Ovary Cell Intellectual Disability Hypomorphic Mutation Diacyl Glycerol Cargo Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.WPI Immunology Frontier Research Center and Research Institute for Microbial DiseasesOsaka UniversitySuitaJapan

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