Imaging Flow Cytometry and High-Throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi
Traditional methods for automated and high-throughput morphological analysis of filamentous fungi can be time-consuming and difficult. Here, two suitable image acquisition methods and subsequent image and data processing steps are presented. The acquisition methods are based on imaging flow cytometry and whole-slide microscopy. Guidelines for developing customized image and data analysis routines for satisfying individual research requirements are presented.
KeywordsAutomated morphological analysis Morphological characterization Imaging flow cytometry Whole-slide microscopy Filamentous fungi
The authors would like to thank Sandoz GmbH for providing the strains and guidance. Financial support was provided by the Austrian research funding association (FFG) under the scope of the COMET program within the research network “Process Analytical Chemistry (PAC)” (contract # 825340).
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