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Single-Use Bioreactors for Animal and Human Cells

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Animal Cell Culture

Part of the book series: Cell Engineering ((CEEN,volume 9))

Abstract

Single-use (SU) bioreactors are being increasingly used in production processes based on animal (i.e. mammalian and insect) and human cells. They are particularly suitable for the production of high-value products on small and medium scales, and in cases where fast and safe production is a requirement. Thus, it is not surprising that SU bioreactors have established themselves for screening studies, cell expansions, and product expressions where they are used for the production of pre-clinical and clinical samples of therapeutic antibodies and preventive vaccines. Furthermore, recent publications have revealed the potential of SU bioreactors for the production of cell therapeutics using human mesenchymal stem cells (hMSCs).

This chapter provides a perspective on current developments in SU bioreactors and their main applications. After briefly introducing the reader to the basics of SU bioreactor technology (terminology, historical milestones and characteristics compared to their reusable counterparts) an overview of the categories of currently available SU bioreactor types is provided. SU bioreactor instrumentation is then examined, before discussing well-established and novel applications of SU bioreactors for animal and human cells. This includes descriptions of the engineering characteristics of often-used types of SU bioreactors, covering wave-mixed, stirred, orbitally shaken systems and fixed-bed systems. In this context, the scaling-up of geometrically and non-geometrically similar SU bioreactors is also addressed.

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Abbreviations

1D:

One-dimensional

2D:

Two-dimensional

3D:

Three-dimensional

bDtBPP:

Bis(2,4-di-tert-butyl phenyl) phosphate

BEVS:

Baculovirus expression vector system

CFD:

Computational fluid dynamics

CHO:

Chinese Hamster Ovary (cells)

CMO:

Contract manufacturing organization

DoE:

Design of experiments

DO:

Dissolved oxygen

EDR:

Energy dissipation rate

EVA:

Ethylene vinyl acetate

hMSCs:

Human mesenchymal stem cells

hADSCs:

Human adipose-derived mesenchymal stem cells

hBM-hMSCs:

Human bone(marrow)-derived mesenchymal stem cells

HPTS:

Hydroxypyrene trisulfonate acid

LED:

Light-emitting diode

mAbs:

Monoclonal antibodies

MDCK:

Madin-Darby Canine Kidney (cells)

MEMS:

Micro-electro-mechanical systems

NK:

Natural killer (cells)

PE:

Polyethylene

PET:

Polyethylene terephthalate

PIV:

Particle image velocimetry

pO2 :

Partial pressure of oxygen

pCO2 :

Partial pressure of carbon dioxide

PTFE:

Polytetrafluoroethylene

PVC:

Polyvinylchloride

QbD:

Quality by Design

RANS:

Reynolds averaged Navier-Stokes (equations)

RFID:

Radio-frequency identification

RT:

Ruston turbine

SBI:

Segment blade impeller

Sf-9:

Spodoptera frugiperda (subclone 9)

SU:

Single-use

TBPP:

Tris(2,4-di-tert-butylphenyl) phosphite

VLP:

Virus-like particle

WCB:

Working cell bank

WVB:

Working virus bank

WFI:

Water for injections

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Acknowledgments

The authors would like to thank M.Sc. Carmen Schirmaier and B.Sc. Valentin Jossen for the experimental work with the hADSCs in the Mobius® CellReady 3 L bioreactor and the MC suspension investigations. Gerhard Greller (Sartorius Stedim Biotech) and Mark Selker (Finesse Solutions, Inc.) we thank for the fruitful discussions.

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  1. Institute of Biotechnology, School of Life Sciences and Facility Management, Zurich University of Applied Sciences, Wädenswil, Switzerland

    Stephan C. Kaiser, Dieter Eibl & Regine Eibl

  2. Technische Universität Berlin, Chair of Chemical and Process Engineering, Berlin, Germany

    Matthias Kraume

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Correspondence to Regine Eibl .

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    Mohamed Al-Rubeai

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Kaiser, S.C., Kraume, M., Eibl, D., Eibl, R. (2015). Single-Use Bioreactors for Animal and Human Cells. In: Al-Rubeai, M. (eds) Animal Cell Culture. Cell Engineering, vol 9. Springer, Cham. https://doi.org/10.1007/978-3-319-10320-4_14

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