Electrochemical Antibody-Aptamer Assay for VEGF Cancer Biomarker Detection

  • Andrea Ravalli
  • Giovanna Marrazza
  • Lourdes Rivas
  • Alfredo De La Escosura-Muniz
  • Arben Merkoci
Conference paper
First Online:
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 268)

Abstract

In this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker.

Keywords

Vascular Endothelial Growth Factor Vascular Endothelial Growth Factor Level Differential Pulse Voltammetry Vascular Endothelial Growth Factor Protein Vascular Endothelial Growth Factor Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Andrea Ravalli
    • 1
  • Giovanna Marrazza
    • 1
  • Lourdes Rivas
    • 2
  • Alfredo De La Escosura-Muniz
    • 2
  • Arben Merkoci
    • 2
  1. 1.Department of Chemistry “Ugo Schiff”University of FlorenceSesto FiorentinoItaly
  2. 2.Nanobioelectronics & Biosensors GroupCIN2 (ICN-CSIC), Catalan Institute of NanotechnologyBellaterraSpain

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