Golgi Apparatus, TGN and Trans Golgi-ER

  • Margit Pavelka
  • Jürgen Roth
Chapter

Abstract

The Golgi apparatus is a highly dynamic organelle that changes its shape and architecture concomitantly with the continuous flow and extensive traffic across its compartments. Chemical fixation is too slow to resolve properly most of the rapid shape changes and moreover in multiple cases, obscures the critical view of ultrastructural details due to artefacts occurring during fixation. These problems and restrictions may be overcome by using cryotechniques and in particular, by employing high pressure freezing for ultrafast immobilisation of cells and their subcompartments. This makes it possible to arrest cellular dynamics in less than half a second. High pressure cryoimmobilisation of membranes and compartments considerably improves the temporal resolution of cellular dynamics and, by preventing artefacts, improves spatial resolution and permits a view that is closer to the in vivo-state than that obtained by chemical fixation.

Keywords

Golgi Apparatus Trans Golgi Network Chemical Fixation Cellular Dynamic Golgi Cisterna 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Bouchet-Marchis C, Starkuviene V, and Grabenbauer M (2008) Golgi apparatus studied in vitreous sections. J Microsc 230: 308CrossRefGoogle Scholar
  2. Griffiths G and Simons K (1986) The trans Golgi network: Sorting at the exit site of the Golgi complex. Science 234: 438CrossRefPubMedGoogle Scholar
  3. Hess MW, Müller M, Debbage PL, Vetterlein M, and Pavelka M (2000) Cryopreparation provides new insight into effects of Brefeldin A on the structure of the HepG2 Golgi apparatus. J Struct Biol 130: 63CrossRefPubMedGoogle Scholar
  4. Jackson CL (2009) Mechanisms of transport through the Golgi complex. J Cell Sci 122: 443CrossRefPubMedGoogle Scholar
  5. Ladinsky MS, Mastronarde DN, McIntosh JR, Howell KE, and Staehelin LA (1999) Golgi structure in three dimensions: Functional insights from the normal rat kidney cell. J Cell Biol 144: 1135CrossRefPubMedGoogle Scholar
  6. Leis A, Rockel B, Andrees L, and Baumeister W (2008) Visualizing cells at the nanoscale. Trends Biochem Sci 34: 60CrossRefPubMedGoogle Scholar
  7. Luci ć V, Leis A, and Baumeister W (2008) Cryo-electron tomography of cells: connecting structure and function. Histochem Cell Biol 130:185CrossRefPubMedGoogle Scholar
  8. Mironov AA and Pavelka M (2008) The Golgi apparatus as a crossroads in intracellular traffic. In: The Golgi apparatus. State of the art 110 years after Camillo Golgi’s discovery (Mironov A and Pavelka M, eds). Wien New York: Springer, p 16Google Scholar
  9. Moor H (1987) Theory and practice of high pressure freezing. In: Cryotechniques in biological electron microscopy (Steinbrecht RA, and Zierold K, eds). Berlin Heidelberg: Springer, p 175Google Scholar

Copyright information

© Springer-Verlag/Wien 2010

Authors and Affiliations

  • Margit Pavelka
    • 1
  • Jürgen Roth
    • 2
  1. 1.Center for Anatomy and Cell Biology, Department of Cell Biology and Ultrastructure ResearchMedical University of ViennaViennaAustria
  2. 2.Yonsei University Graduate School, World Class University Program, Department of Biomedical ScienceYonsei UniversitySeoulKorea

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