Neuronal differentiation and long-term culture of the human neuroblastoma line SH-SY5Y
Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder in industrialized countries. Present cell culture models for PD rely on either primary cells or immortal cell lines, neither of which allow for long-term experiments on a constant population, a crucial requisite for a realistic model of slowly progressing neurodegenerative diseases.
KeywordsDopaminergic neurons mitotic inhibitors neuronal differentiation neuronal markers perfusion culture retinoic acid
Unable to display preview. Download preview PDF.
- Cajal SR y (1928) Degeneration and regeneration of the nervous system. University Press, LondonGoogle Scholar
- Encinas M, Iglesias M, Liu Y, Wang H, Muhaisen A, Cena V, Gallego C, Cornelia JX (2000) Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells. J Neurochem 75: 991–1003PubMedCrossRefGoogle Scholar
- Rasband WS (2006) Image J. U. S. National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/