Intrastriatal transplantation of mouse bone marrow-derived stem cells improves motor behavior in a mouse model of Parkinson’s disease

  • D. Offen
  • Y. Barhum
  • Y.-S. Levy
  • A. Burshtein
  • H. Panet
  • T. Cherlow
  • E. Melamed
Conference paper

DOI: 10.1007/978-3-211-73574-9_16

Part of the Journal of Neural Transmission. Supplementa book series (NEURALTRANS, volume 72)
Cite this paper as:
Offen D. et al. (2007) Intrastriatal transplantation of mouse bone marrow-derived stem cells improves motor behavior in a mouse model of Parkinson’s disease. In: Gerlach M., Deckert J., Double K., Koutsilieri E. (eds) Neuropsychiatric Disorders An Integrative Approach. Journal of Neural Transmission. Supplementa, vol 72. Springer, Vienna

Abstract

Strategies of cell therapy for the treatment of Parkinson’s disease (PD) are focused on replacing damaged neurons with cells to restore or improve function that is impaired due to cell population damage. In our studies, we used mesenchymal stromal cells (MSCs) from mouse bone marrow. Following our novel neuronal differentiation method, we found that the basic cellular phenotype changed to cells with neural morphology that express specific markers including those characteristic for dopaminergic neurons, such as tyrosine hydroxylase (TH). Intrastriatal transplantation of the differentiated MSCs in 6-hydroxydopamine-lesioned mice led to marked reduction in the amphetamine-induced rotations. Immunohistological analysis of the mice brains four months post transplantation, demonstrated that most of the transplanted cells survived in the striatum and expressed TH. Some of the TH positive cells migrated toward the substantia nigra. In conclusion, transplantation of bone marrow derived stem cells differentiated to dopaminergic-like cells, successfully improved behavior in an animal model of PD suggesting an accessible source of cells that may be used for autotransplantation in patient with PD.

Keywords

Multipotent mesenchymal stromal cells (MSCs) dopamine dopaminergic neurons Parkinson’s disease (PD) stem cells 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • D. Offen
    • 1
    • 2
  • Y. Barhum
    • 2
  • Y.-S. Levy
    • 2
  • A. Burshtein
    • 2
  • H. Panet
    • 2
  • T. Cherlow
    • 2
  • E. Melamed
    • 2
    • 3
  1. 1.Felsenstein Medical Research Center, Beilinson CampusTel Aviv University, Rabin Medical CenterIsrael
  2. 2.Laboratory of Neurosciences, Felsenstein Medical Research Center, Sackler Faculty of MedicineTel Aviv University, Tel AvivIsrael
  3. 3.Department of NeurologyRabin Medical CenterPetah TiqvaIsrael

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