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Detection and production of nitric oxide

  • Chapter
Analysis of Free Radicals in Biological Systems

Abstract

The methods most commonly used to detect or measure nitric oxide are summarized in this chapter. Measurement of nitrite produced from •NO by activated human monocytes or by synthetic thionitrites is described in detail. Special emphasis is made on the spectroscopic assays for •NO, including spectrophotometry and EPR spectroscopy. The assays are based on the reaction between a metal center and •NO. A very specific EPR test, based on the reversible coupling reaction between •NO and the tyrosyl radical of ribonucleotide reductase, is reported.

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Author information

Authors and Affiliations

  1. Groupe de Recherches des Pathologies Oxydatives, Centre Hospitalier Universitaire, BP217, F-38043, Grenoble Cedex 9, France

    C. Garrel

  2. Laboratoire d’Etudes Dynamiques et Structurales de la Sélectivité, Université Joseph Fourier, BP53, F-38041, Grenoble, France

    J.-L. Decout & M. Fontecave

Authors
  1. C. Garrel
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  2. J.-L. Decout
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  3. M. Fontecave
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Editor information

Editors and Affiliations

  1. Laboratoire de Biochimie C, CHU Albert Michallon, BP 217, F-38043, Grenoble Cédex 9, France

    A. E. Favier

  2. CEA/Département de Recherche Fondamentale sur la Matière, Condensée, SESAM/LAN, 17, rue des Martyrs, F-38054, Grenoble Cédex 9, France

    J. Cadet

  3. Biophysics Research Institute, Medical College of Wisconsin, 8701 Watertown Plank Road, PO Box 26509, Milwaukee, WI, 53226-0509, USA

    B. Kalyanaraman

  4. Laboratoire de Biochimie Recherche, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    M. Fontecave

  5. Laboratoire de Chimie Biomimétique LEDSS II, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    J.-L. Pierre

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Garrel, C., Decout, JL., Fontecave, M. (1995). Detection and production of nitric oxide. In: Favier, A.E., Cadet, J., Kalyanaraman, B., Fontecave, M., Pierre, JL. (eds) Analysis of Free Radicals in Biological Systems. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9074-8_20

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  • DOI: https://doi.org/10.1007/978-3-0348-9074-8_20

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-9895-9

  • Online ISBN: 978-3-0348-9074-8

  • eBook Packages: Springer Book Archive

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