Analysis of Free Radicals in Biological Systems pp 225-236 | Cite as
Measurement of one of the features of apoptosis: DNA fragmentation
Chapter
Summary
Unlike necrosis. apoptosis was observed to proceed in an orderly and controlled manner that appeared to follow a defined program. DNA fragmentation is one of the features of apoptosis in lymphocytes. Most chemical and physical treatments capable of inducing apoptosis are also known to evoke oxidative stress. Dexamethasone can induce apoptosis and trigger a quantitative increase in lipid peroxidation. This work shows two means to measure with flow cytometry DNA fragmentation in mouse thymocytes after dexamethasone treatment. Alternatively. electrophoretic analysis of thymocyte lysate DNA is performed.
Keywords
Dexamethasone Treatment Thymocyte Apoptosis Mouse Thymocyte Flow Cytometric Method Hypodiploid Cell
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