Primary cell cultures from fish in ecotoxicology

  • Gilles Monod
  • Alain Devaux
  • Yves Valotaire
  • Jean-Pierre Cravedi
Chapter
Part of the EXS book series (EXS, volume 86)

Summary

Fish cell cultures from fish are used in studies on the fate and effects of xenobiotics at the cellular level. So far, special attention has been paid to primary cultures of hepatocytes. This cell type has been particularly useful for studies on biotransformation of xenobiotics. Among biotransformation enzymes, a number of studies have been devoted to cytochrome P4501A due to its inducibility by ecotoxicologically relevant pollutants. Biomarkers of DNA damage, i.e., DNA adducts and single strand breaks, have also been investigated. Furthermore, primary cultures of fish hepatocytes appear as promising models for studies on the effects of xenobiotics on critical steps of fish reproductive physiology. All the data are discussed in an ecotoxicological perspective.

Keywords

Rainbow Trout Primary Cell Culture Fish Cell Fish Cell Line Cytochrome P450 Content 
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Copyright information

© Springer Basel AG 1998

Authors and Affiliations

  • Gilles Monod
    • 1
  • Alain Devaux
    • 2
  • Yves Valotaire
    • 3
  • Jean-Pierre Cravedi
    • 4
  1. 1.Institut National de la Recherche AgronomiqueUnité d’Écotoxicologie AquatiqueRennes CedexFrance
  2. 2.Laboratoire des Sciences de l’EnvironnementÉcole Nationale des Travaux Publics de l’ÉtatVaulx-en Velin CedexFrance
  3. 3.Équipe d ‘Endocrinologie Moléculaire de la ReproductionUPRES-A CNRS 6026Rennes CedexFrance
  4. 4.Laboratoire des XénobiotiquesInstitut National de la Recherche AgronomiqueToulouse CedexFrance

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