Microsystem Technology pp 241-254 | Cite as
Using oligonucleotide probe arrays to access genetic diversity
Chapter
Abstract
The goal of the Human Genome Project is to sequence all 3 billion base pairs of the human genome. Progress in this has been rapid; GenBank® finished April1997 with 843 million bases of sequence. The challenge to the scientific community is to understand the biological relevance of this genetic information. In most cases the sequence being generated for any single region of the genome represents the genotype of a single individual. A complete understanding of the function of specific genes and other regions of the genome and their role in human disease and development will only become apparent when the sequence of many more individuals is known.
Keywords
Human Immunodeficiency Virus Type Human Genome Project Oligonucleotide Array Probe Array Target Nucleic Acid
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
- 1.Bains W, Smith, GC (1988) A novel method for nucleic acid sequence determination. J Theor Biol 135: 303 - 307PubMedCrossRefGoogle Scholar
- 2.Drmanac R, Labat I, Brukner I, Crkvenjakov R (1989) Sequencing of megabase plus DNA by hybridization: theory of the method. Genomics4: 114–128PubMedCrossRefGoogle Scholar
- 3.Fodor SPA, Lipshutz RJ, Huang X (1993) DNA sequencing by hybridization, p 3–9. Proceedings of The Robert A. Welch Foundation 37th Conference on Chemical Research — 40 Years of the DNA Double Helix. Robert A. Welch Foundation, HoustonGoogle Scholar
- 4.Fodor SPA, Rava R, Huang XC, Pease AC, Holmes CP, Adams CL (1993) Multiplexed biochemical assays with biological chips. Nature364: 555–556PubMedCrossRefGoogle Scholar
- 5.Fodor SPA, Read JL, Pirrung MC, Stryer L, Lu AT, Solas D (1991) Light-directed, spa-tially addressable parallel chemical synthesis. Science251: 767–773PubMedCrossRefGoogle Scholar
- 6.Kozal MJ, Merigan TC (1993) HIV resistance to dideoxynucleoside inhibitors. Infect Dis Clin Practice2: 247–253CrossRefGoogle Scholar
- 7.Land S, McGavin C, Lucas R, Birch C (1992) Incidence of zidovudine-resistant human immunodeficiency virus isolated from patients before, during, and after therapy. J Infect Dis166: 1139–1142PubMedCrossRefGoogle Scholar
- 8.Larder BA, Kemp SD (1989) Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT). Science246: 1155–1157PubMedCrossRefGoogle Scholar
- 9.Lipshutz R, Fodor SPA (1994) Advanced DNA sequencing technologies. Curr Opin Struct Biol4: 376–380CrossRefGoogle Scholar
- 10.Lysov YP, Florentiev VL, Khorlin AA, Khrapko KV, Shik VV, Mirzabekov AD (1988) DNA sequencing by hybridization with oligonucleotides. A novel method. Dokl Acad Sci USSR 303: 1508–1511Google Scholar
- 11.Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74: 560–664PubMedCrossRefGoogle Scholar
- 12.Pease AC, Solas D, Sullivan EJ, Cronin MT, Holmes CP, Fodor SPA (1993) Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc Natl Acad Sci USA 91: 5022–5026CrossRefGoogle Scholar
- 13.Richman DD (1995) Protease uninhibited. Nature 374: 494–495PubMedCrossRefGoogle Scholar
- 14.Richman DD, Shih CK, Lowy I, Rose J, Prodanovich P, Goff S, Griffin J (1991) Human immunodeficiency virus type 1 mutants resistant to nonnucleoside inhibitors of reverse transcriptase arise in tissue culture. Proc Natl Acad Sci USA88: 11241–11245PubMedCrossRefGoogle Scholar
- 15.Sanger F, Nicklen S, Coulson R (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74: 5463–5467PubMedCrossRefGoogle Scholar
- 16.Southern E, Maskos U, Elder R (1992) Hybridization with oligonucleotide arrays. Genomics13: 1008–1017PubMedCrossRefGoogle Scholar
- 17.Kozal MJ, Shah N, Shen N, Yang R, Fucini R, Merrigan T, Richman D, Morris D, Hubbell E, Chee MS, Gingeras TG (1996) Extensive polymorphisms observed in HIV-1 clade B protease gene using high-density oligonucleotide arrays. Nature Medicine7: 753–759CrossRefGoogle Scholar
- 18.Lockhart DJ, Dong H, Byrne MC, Follettie M, Gallo MV, Chee MS, Mittmann M, Wang C, Kobayashi M, Horton H, Brown EL (1996) Expression monitoring by hybridization to high-density oligonucleotide arrays. Nature Biotech14: 1675–1680CrossRefGoogle Scholar
- 19.Hacia JG, Brody LC, Chee MS, Fodor SPA, Collins FS (1996) Detection of heterozygous mutations in BRCA1 using high-density oligonucleotide arrays and two-colour fluorescence analysis. Nature Genetics14: 441–447PubMedCrossRefGoogle Scholar
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