Paternity testing of endangered species of birds by DNA fingerprinting with non-radioactive labelled oligonucleotide probes

  • J. Mêthé
  • C. Eisenmann
  • A. Seitz
Part of the Progress in Systems and Control Theory book series (EXS)

Summary

In the last years, DNA fingerprinting became the most powerful tool for identification and paternity testing in man. The success of this method encouraged the German Federal Ministry of Environment, Natural Protection and Reactor Safety to apply DNA fingerprinting in the field of protection of endangered species of birds, such as birds of prey or parrots. In the last three years, we received more than 400 blood and tissue samples of 23 species of birds of prey or parrots, most of them obtained by confiscation, to establish paternity and legal breeding success. We used digoxigenated oligonucleotide probes, mainly (GGAT)4 and (GACA)4 for hybridization. In most cases of confiscated families of birds, paternity testing showed exclusions of nestlings.

Keywords

Endangered Species Natural Protection Short Exposure Time Snow Goose Paternity Testing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arctander P (1988) Comparative studies of avian DNA by restriction fragment length polymorphisms analysis: convenient procedures on blood samples from live birds. J Orn 129: 205–216CrossRefGoogle Scholar
  2. Burke T, Bruford MW (1987) DNA fingerprinting in birds. Nature 327: 149–152CrossRefGoogle Scholar
  3. Epplen JT, Kammerbauer D, Steimle V, Zischler H, Alber E, Andreas A, Hala K, Nanda I, Schmid M, Rieß O, Weising K (1989) Methodology and application of oligonucleotide fingerprinting including characterization of individual hypervariable loci. In: Radola BJ (ed) Electrophoresis Forum 89, Technische Universität München, pp 175–186Google Scholar
  4. Gyllenstein UB, Jakobsson S, Temrin H, Wilson AC (1989) Nucleotide sequence and genomic organization of bird minisatellites. Nucleic Acids Res 17: 2203–2214CrossRefGoogle Scholar
  5. Hill WG (1987) DNA fingerprints applied to animal and bird populations. Nature 327: 98–99CrossRefGoogle Scholar
  6. Jeffreys AJ, Wilson V, Thein SL (1985a) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314: 67–73CrossRefGoogle Scholar
  7. Jeffreys AJ, Wilson V, Thein SL (1985b) Individual-specific ‘fingerprints’ of human DNA. Nature 316: 76–79CrossRefGoogle Scholar
  8. Jeffreys AJ, Wilson V, Thein SL, Weatherall DJ, Ponder BAJ (1986) DNA ‘fingerprints’ and segregation analysis of multiple markers in human pedigrees. Am J Hum Genet 39: 11–24Google Scholar
  9. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18: 1–5CrossRefGoogle Scholar
  10. Longmire JL, Ambrose RE, Brown NC, Cade TJ, Maechtle TL, Seegar WS, Ward FP, White CM (1991) Use of sex-linked minisatellite fragments to investigate genetic differentiation and migration of North American populations of the peregrine falcon (Falco peregrinus)In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA fingerprinting: approaches and applications, 1st ed. Birkhäuser Verlag, Basel Boston Berlin, pp 217–229CrossRefGoogle Scholar
  11. Mêthé J, Wolfes R, Seitz A (1991) DNA fingerprinting for the protection of endangered species. In: Berghaus G, Brinkmann B, Rittner C, Staak M (eds) DNA-technology and its forensic application, 1st ed. Springer Verlag, Berlin, pp 170–171CrossRefGoogle Scholar
  12. Miller SA, Dykes DO, Polesky HT (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215CrossRefGoogle Scholar
  13. Quinn TW, Quinn JS, Cooke F, White BN (1987) DNA marker analysis detects multiple maternity and paternity in single broods of the lesser snow goose. Nature 326: 392–394CrossRefGoogle Scholar
  14. Schacker U, Schneider PM, Holtkamp B, Bohnke E, Fimmers R, Sonneborn HH, Ritter C (1990) Isolation of the DNA minisatellite probe MZ1.3 and its application to DNA fingerprinting analysis. Forensic Sci Int 44: 209–224CrossRefGoogle Scholar
  15. Weiss ML (1989) DNA fingerprints in physical anthropology. Am J Hum Biol 1: 567–579CrossRefGoogle Scholar
  16. Wetton JH, Carter RE, Parkin DT, Walters D (1987) Demographic study of a wild house sparrow population by DNA fingerprinting. Nature 327: 147–149CrossRefGoogle Scholar
  17. Wolfes R, Mêthé J, Seitz A (1991) Forensics of birds of prey by DNA fingerprinting with 32P-labeled oligonucleotide probes. Electrophoresis 12: 175–180CrossRefGoogle Scholar

Copyright information

© Springer Basel AG 1993

Authors and Affiliations

  • J. Mêthé
    • 1
  • C. Eisenmann
    • 1
  • A. Seitz
    • 1
  1. 1.Institute of ZoologyUniversity of MainzMainzGermany

Personalised recommendations