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HPAE-PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins Electrotransferred onto Polyvinylidene Fluoride Membranes

  • Michael Weitzhandler
  • Nebojsa Avdalovic
Part of the BioMethods book series (BIOMETHODS)

Abstract

Protein glycosylation is a major post-translational modification that generates a potentially large group of glycoforms from a single polypeptide chain (1). Over 50% of catalogued protein sequences contain the requisite sequence for N-glycosylation (AsnXxxSer(Thr); Xxx ≠ Pro) (2), and any Ser or Thr can potentially be glycosylated (3). Since glycosylation can play an important role in the biological and pharmacologic activity of the glycoprotein, there is increasing interest in identifying and characterizing the oligosaccharide moieties of glycoproteins.

Keywords

Sialic Acid Amino Sugar Carbohydrate Analysis Monosaccharide Analysis Membrane Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Rademacher TW, Parekh RB and Dwek RA (1988) Ann. Rev. Biochem. 57: 785.PubMedCrossRefGoogle Scholar
  2. 2.
    George DG, Barker WC and Hunt LT (1986) Nucleic Acids Res. 14: 11.PubMedCrossRefGoogle Scholar
  3. 3.
    Sadler JE (1984) In: V Ginsburg and P W Robbins (eds), Biology of Carbohydrates, p. 199, John Wiley, New York.Google Scholar
  4. 4.
    Matsudaira P (1987) J. Biol Chem. 262: 10035.PubMedGoogle Scholar
  5. 5.
    Rohringer R and Holden DW (1985) Anal Biochem. 144: 118.PubMedCrossRefGoogle Scholar
  6. 6.
    Kijomoto-Ochiai S, Katagiri YU and Ochiai H (1985) Anal. Biochem. 147: 222.CrossRefGoogle Scholar
  7. 7.
    Haselbeck A, Schickaneder E, von der Eitz H and Hösel W (1990) Anal Biochem. 191: 25.PubMedCrossRefGoogle Scholar
  8. 8.
    Segrest JP and Jackson RL (1972) Methods Enzymol 28: 54.CrossRefGoogle Scholar
  9. 9.
    Eckhardt AE, Hayes CE and Goldstein IJ (1976) Anal Biochem. 73: 192.PubMedCrossRefGoogle Scholar
  10. 10.
    Weitzhandler M, Kadlecek D, Avdalovic N, Forte JG, Chow D and Townsend RR (1993) Anal. Biol Chem. 268: 5121.Google Scholar
  11. 11.
    Townsend RR and Hardy MR (1991) Glycobiology 1: 139.PubMedCrossRefGoogle Scholar
  12. 12.
    Spellman MW (1990) Anal Chem. 62: 1714.PubMedCrossRefGoogle Scholar
  13. 13.
    Manzi A, Diaz S and Varki A (1990) Anal. Biochem. 188: 20.PubMedCrossRefGoogle Scholar
  14. 14.
    Pfeiffer G, Geyer H, Geyer R, Kalsner I and Wendorf P (1990) Biomed. Chromatogr. 4: 183.CrossRefGoogle Scholar
  15. 15.
    Townsend RR, Atkinson PH and Trimble RB (1991) Carbohydr. Res. 215: 211.PubMedCrossRefGoogle Scholar
  16. 16.
    Townsend RR, Hardy MR, Cumming DA, Carver J.P and Bendiak B (1989) Anal. Biochem. 182: 1.PubMedCrossRefGoogle Scholar
  17. 17.
    Cooper GA and Rohrer J. (1995) Anal. Biochem. 226: 182.PubMedCrossRefGoogle Scholar
  18. 18.
    Reddy GPand Bush CA(1991) Anal. Biochem. 198: 278.Google Scholar
  19. 19.
    Hardy MR, Townsend RR and Lee YC (1988) Anal. Biochem. 170: 54.PubMedCrossRefGoogle Scholar
  20. 20.
    Townsend RR, Hardy MH, Hindsgaul O and Lee YC (1988) Anal. Biochem. 174: 459.PubMedCrossRefGoogle Scholar
  21. 21.
    Laemmli UK (1970) Nature 227: 680.PubMedCrossRefGoogle Scholar
  22. 22.
    Harnes BD (1990) A Practical Approach, In: B. D. Harnes D. and Rickwood (eds) Gel Electrophoresis of Proteins, vol. 1, p. 148, IRL Press at Oxford University Press, Oxford.Google Scholar
  23. 23.
    Andersen DC, Goochee CF, Cooper G and Weitzhandler M (1994) Glycobiology 4: 459.PubMedCrossRefGoogle Scholar

Copyright information

© Birkhäuser Verlag Basel 1997

Authors and Affiliations

  • Michael Weitzhandler
  • Nebojsa Avdalovic

There are no affiliations available

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