Metallothionein-Determination in Biological Materials: Interlaboratory Comparison of 5 Current Methods

  • H. H. Dieter
  • L. Müller
  • J. Abell
  • K. H. Summer
Part of the Experientia Supplementum book series (EXS, volume 52)

Abstract

Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after “Kjeldahl-digestion”. Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.

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References

  1. 1.
    Chen, R.W., and Ganther, A.E. (1975) Environ. Physiol. Biochem. 5, 378–388Google Scholar
  2. 2.
    Piotrowski, J.R., Bolanowska, W., and Sapota, A. (1973) Acta Biochim. Pol. 20, 207–215.Google Scholar
  3. 3.
    Onosaka, S., and Cherian, M.G. (1982) Toxicol. Appl. Pharmacol. 63, 270–274.CrossRefGoogle Scholar
  4. 4.
    Olafson, R.W., and Sim, R.G. (1979) Anal. Biochem. 100, 343–351.CrossRefGoogle Scholar
  5. 5.
    Menzel, H., Abel, J., and Merfort, F. (1982) NaunynSchmiedeberg’s Arch. Pharmacol. Suppl. 319, R26.Google Scholar
  6. 6.
    Webb, M. (1979) In: “The Chemistry, Biochemistry and Biology of Cadmium”, M. Webb ed., Elsevier/North Holland, Amsterdam, pp. 195–266.Google Scholar
  7. 7.
    Winge, D.R., and Miklossy, K.A. (1982) J. Biol. Chem. 257, 3471–3476.Google Scholar
  8. 8.
    Klausner, St., Kägi, J.H.R., and Wilson, K.J. (1983) Biochem. J. 209, 71–80.Google Scholar
  9. 9.
    Kotsonis, F.N., and Klaassen, C.D. (1977) Toxicol. Appl. Pharmacol. 42, 583–588.CrossRefGoogle Scholar
  10. 10.
    Patierno, S.R., Pellis, N.R., Evans, R.M., and Costa, M. (1983) Life Sci. 32, 1629–1636.CrossRefGoogle Scholar
  11. 11.
    Waalkes, M.P., Garvey, S.J., and Klaassen, C.D. (1985) Toxicol. Appl. Pharmacol. 79, 524–527.CrossRefGoogle Scholar
  12. 12.
    Kimura, M., Otaki, N., and Imano, M. (1979) In: “Metallothioneins”, J.H.R. Kägi and M. Nordberg eds., Birkhäuser Verlag, Basel, pp. 163–168.CrossRefGoogle Scholar
  13. 13.
    Burnette, W.N. (1981) Anal. Biochem. 112, 195–203.CrossRefGoogle Scholar
  14. 14.
    Fels, G., and Veatch, R. (1959) Anal. Chem. 31, 451–452.CrossRefGoogle Scholar
  15. 15.
    Eaton, D.L., and Toal, B. (1982) Toxicol. Appl. Pharmacol. 66, 134–142.CrossRefGoogle Scholar
  16. 16.
    Day, F.A., Funk, A.E., and Brady, F.O. (1984) Chem. biol. Interact. 50, 159–174.CrossRefGoogle Scholar
  17. 17.
    Carlsson, J., Drevin, H., and Axen, R. (1978) Biochem. J. 173, 723–737.Google Scholar
  18. 18.
    Bolton, A.E., and Hunter, W.M. (1973) Biochem. J. 133, 529–539.Google Scholar
  19. 19.
    Sokolowski, G., and Weser, U. (1975) Hoppe-Seyler’s Z. Physiol. Chem. 356, 1715–1726.CrossRefGoogle Scholar
  20. 20.
    Ellman, G.L. (1959) Arch. Biochem. Biophys. 82, 70–77.CrossRefGoogle Scholar
  21. 21.
    Beutler, E., Duron, O., and Kelly, B.M. (1963) J. Lab. Clin. Med. 61, 882–888.Google Scholar
  22. 22.
    Heilmaier, C.E., and Summer, K.H. (1985) Arch. Toxicol. 256, 247–251.CrossRefGoogle Scholar
  23. 23.
    Waalkes, P.M., and Poirier, L.A. (1985) Toxicol. Appl. Pharmacol. 79, 47–53.CrossRefGoogle Scholar
  24. 24.
    Chellman, G.J., Shaikh, Z.A., Baggs, R.B., and Diamond, G.L. (1985) Toxicol. Appl. Pharmacol. 79, 511–523.CrossRefGoogle Scholar
  25. 25.
    Onosaka, S., and Cherian, M.G. (1981) Toxicology 22, 91–101.CrossRefGoogle Scholar
  26. 26.
    Kotsonis, F.N., and Klaassen, C.D. (1979) Toxicol. Appl. Pharmacol. 51, 19–27.CrossRefGoogle Scholar
  27. 27.
    Abel, J., unpublished data.Google Scholar

Copyright information

© Springer Basel AG 1987

Authors and Affiliations

  • H. H. Dieter
    • 1
  • L. Müller
    • 1
  • J. Abell
    • 2
  • K. H. Summer
    • 3
  1. 1.Institut für ToxikologieDüsseldorf 1Germany
  2. 2.Institute of Environmental HygieneDüsseldorfGermany
  3. 3.Institute of Toxicology and BiochemistryGSF Research CenterNeuherbergGermany

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