Organelle Transfer, Sorting Out, Recombination

Techniques for organelle transfer and plasmon transfer: The use of albino mutants, X-rays, and hormones togetherwith different ratios of donor and recipient protoplasts (Pl. Sci. Lett. 14: 49–55, 1979) were mentioned. Also successful experiments with enucleated protoplasts (MGG 185: 211–215, 1982 and poster PE1, this conference), and selective chloroplast (cp) markers (MGG 189: 365–369, 1983) were mentioned. Other techniques such as microinjection of organelles were mentioned as desired techniques to be adopted for plant protoplast manipulation. The rapid progress in transfer of specific cytoplasmic genomes and cytoplasmic characters was pointed out, namely chloroplasts (cp), mitochondria (mt) and cytoplasmic male sterility (cms).

Analysis of somatic hybrids and cybrids: (a) Genetic analysis was possible in cases of clear genetic markers, such as albinism and cms. (b) Biochemical and molecular analysis included so far polypeptide and enzyme analyses, restriction enzymes and Southern blot hybridization with various probes.