Effect of Quercetin on Cell Proliferation and Heat Shock Protein Synthesis Induction in K562 Erythroleukemia

  • G. Elia
  • M. G. Santoro
Chapter
Part of the MCBU Molecular and Cell Biology Updates book series (MCBU)

Summary

Quercetin, an inhibitor of 5-lipoxygenase (5-Lox), has been shown to inhibit heat shock protein (HSP) synthesis. Since the antiproliferative activity of prostaglandins of the A type (PGA) has been associated with the induction of HSP70 synthesis, we have investigated the effect of quercetin on the control of cell proliferation by PGA1 in K562 cells. The results show that quercetin has no effect on PGA1-induced block of cell proliferation. However, analysis of the effect of quercetin on HSP70 induction after PGA1-treatment revealed that the presence of the drug only delayed but did not reduce HSP70 synthesis in these cells. In fact, after an initial 2–3 h delay, HSP70 synthesis continued up to 20 h at much higher levels than in the PGA1-treated control. The results suggest that in K562 cells the interaction of quercetin with the complex constituted by the cis-acting HSE in the promoter region of the gene and a trans-acting cytoplasmic HSF is limited in time, and that cells are able to regain a normal heat shock response in a few hours after exposure to the drug. The results also indicate that quercetin itself is a potent inhibitor of K562 cell proliferation.

Keywords

Heat Shock Protein K562 Cell Heat Shock Response Heat Shock Factor Heat Shock Transcription Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Basel AG 1993

Authors and Affiliations

  • G. Elia
    • 1
  • M. G. Santoro
    • 1
    • 2
  1. 1.Institute of Experimental MedicineCNRRomeItaly
  2. 2.Department of Experimental MedicineUniversity of L’AquilaL’AquilaItaly

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