Structural Analysis of the Glucocorticoid Receptor Protein

  • Jan Carlstedt-Duke
  • Per-Erik Strömstedt
  • Karin Dahlman
  • Carol Rae
  • Anders Berkenstam
  • Janet Hapgood
  • Hans Jörnvall
  • Jan-Åke Gustafsson
Chapter
Part of the Birkhäuser Congress Reports Life Sciences book series (ALS)

Summary

The glucocorticoid receptor protein consists of at least three functional domains. Two of the domains are responsible for the binding of the two different ligands, steroid and DNA, in a coordinated manner. Probing of the structure of the steroid-binding domain by affinity-labelling and radiosequence analysis has identified three steroid-binding amino acid residues in the rat glucocorticoid receptor and two in the human progestin receptor. These residues occur within a hydrophobic surface on this domain. One of the steroid-binding amino acids in the progestin receptor corresponds to the equivalent residue previously identified in the glucocorticoid receptor. The second amino acid identified did not bind steroid in the glucocorticoid receptor. The DNA-binding domain of the human glucocorticoid receptor has been expressed at a high level in E. coli. This protein binds DNA with the same specificity as the intact receptor protein. Two subunits bind to each specific binding site on DNA. The structure and DNA-binding properties of this domain are metal-dependent.

Keywords

Glucocorticoid Receptor Cleavage Method Triamcinolone Acetonide Triamcinolone Acetonide Limited Proteolysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Basel AG 1989

Authors and Affiliations

  • Jan Carlstedt-Duke
    • 1
    • 2
  • Per-Erik Strömstedt
    • 1
  • Karin Dahlman
    • 1
    • 2
  • Carol Rae
    • 1
  • Anders Berkenstam
    • 1
  • Janet Hapgood
    • 1
  • Hans Jörnvall
    • 2
    • 3
  • Jan-Åke Gustafsson
    • 1
    • 2
  1. 1.Department of Medical NutritionHuddinge University Hospital F69HuddingeSweden
  2. 2.Center for BioTechnologyHuddinge University Hospital F69HuddingeSweden
  3. 3.Department of Chemistry, Karolinska InstitutetHuddinge University Hospital F69HuddingeSweden

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