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Role of Ionic Liquids in the Enzyme Stabilization: A Case Study with Trichoderma Ressie Cellulase

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Multifaceted Protocols in Biotechnology, Volume 2

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Abstract

This chapter discusses the stabilization of Trichoderma ressie cellulase (Tri-Cel) in ionic liquids (ILs) to enable the in situ hydrolysis of cellulosic and lignocellulosic substances. It is well recognized that enzymes tend to lose their activities in ILs, but several methods have been used to increase or minimize the loss of activity in ILs. In this study, cellulase was therefore tested in several ILs. This approach opens an insight for further studies to discover more about the effects of ILs on cellulase and their interactions in the aqueous system. It can also offer successful manufacturing and processing of different biomass biofuels.

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Acknowledgements

The author would like to acknowledge the Ministry of Education Malaysia for awarding the FRGS Grant (Grant no. FRGS-13-088-0329) and the Research Management Centre, IIUM for the grant [RMCG20-021-0021].

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Authors and Affiliations

  1. International Institute for Halal Research and Training (INHART), IIUM, Jalan Gombak, Malaysia

    Amal A. M. Elgharbawy

  2. Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Kuala Lumpur, 50728, Malaysia

    Md Zahangir Alam, Nassereldeen Ahmad Kabbashi & Parveen Jamal

  3. Chemical Engineering Department, Universiti Teknologi PETRONAS, Seri Iskandar, Malaysia

    Muhammad Moniruzzaman

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  1. Amal A. M. Elgharbawy
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  2. Md Zahangir Alam
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  3. Muhammad Moniruzzaman
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  4. Nassereldeen Ahmad Kabbashi
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  5. Parveen Jamal
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Correspondence to Amal A. M. Elgharbawy .

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Editors and Affiliations

  1. International Institute for Halal Research and Training, International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Azura Amid

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Elgharbawy, A.A.M., Alam, M.Z., Moniruzzaman, M., Kabbashi, N.A., Jamal, P. (2021). Role of Ionic Liquids in the Enzyme Stabilization: A Case Study with Trichoderma Ressie Cellulase. In: Amid, A. (eds) Multifaceted Protocols in Biotechnology, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-030-75579-9_2

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