Skip to main content
SpringerLink
Log in

3D-Bioprinting

  • Chapter
  • First Online:
Basic Concepts on 3D Cell Culture

Part of the book series: Learning Materials in Biosciences ((LMB))

What You Will Learn in This Chapter

Bioprinting is a digital biofabrication method that is applied to generate complex and heterogenic 3D cell culture systems and living tissues. It adopts the concept of additive manufacturing to replicate the macroscopic anatomy of the tissue of interest by layer-wise printing of living cells embedded in a hydrogel matrix. The printed precursor structures are cultivated for several weeks in dedicated bioreactors, on-chip platforms, or host organisms to mature and form biofunctional tissue units.

This chapter will give you a detailed overview of 3D-bioprinting technology, its challenges as well as its current and future applications. Following a brief introduction, this chapter provides a step-by-step guide through individual operations throughout the bioprinting process chain.

Beginning with the generation of printable 3D-datasets obtained from medical imaging modalities, such as computed tomography (CT) and magnetic resonance imaging (MRI), and computer-aided design (CAD), this chapter discusses the formulation of bioinks from natural and synthetic origins. Holistically, this chapter discusses different bioprinting methods and mechanisms, ranging from stereolithography and microextrusion to laser-assisted and microvalve-based drop-on-demand printing.

This chapter closes with an overview of current and future applications of 3D-bioprinting for clinical purposes, such as implants, tissue substitutes, in vitro models, and organs-on-a-chip.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 69.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 89.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Langer R, Vacanti JP. Science. 1993;260:920.

    Article  CAS  PubMed  Google Scholar 

  2. Atala A, Bauer SB, Soker S, Yoo JJ, Retik AB. Lancet. 2006;367:1241.

    Article  PubMed  Google Scholar 

  3. Weber M, Gonzalez de Torre I, Moreira R, Frese J, Oedekoven C, Alonso M, Rodriguez Cabello CJ, Jockenhoevel S, Mela P. Tissue Eng Part C Methods. 2015;21:832.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Nieponice A, Soletti L, Guan J, Deasy BM, Huard J, Wagner WR, Vorp DA. Biomaterials. 2008;29:825.

    Article  CAS  PubMed  Google Scholar 

  5. Wilson WC, Boland T. Anat Rec A Discov Mol Cell Evol Biol. 2003;272:491.

    Article  PubMed  Google Scholar 

  6. Pandey PM, Reddy NV, Dhande SG. Int J Mach Tools Manuf. 2003;43:61.

    Article  Google Scholar 

  7. Ding D, Pan Z, Cuiuri D, Li H, van Duin S. Advanced design for additive manufacturing: 3d slicing and 2d path planning. In: New trends 3D print. Rijeka: InTech; 2016.

    Google Scholar 

  8. Szilvśi-Nagy M, Mátyási G. Math Comput Model. 2003;38:945.

    Article  Google Scholar 

  9. Wu T, Cheung EHM. Int J Adv Manuf Technol. 2005;29:1143.

    Article  Google Scholar 

  10. Eragubi M. Int J Innov Manag Technol. 2013;4:410.

    Google Scholar 

  11. Petitjean C, Dacher J-N. Med Image Anal. 2011;15:169.

    Article  PubMed  Google Scholar 

  12. Heimann T, Meinzer H-P. Med Image Anal. 2009;13:543.

    Article  PubMed  Google Scholar 

  13. Blaeser A, Heilshorn SC, Duarte Campos DF. Gels. 2019;5:29.

    Article  CAS  PubMed Central  Google Scholar 

  14. Zhang YS, Yue K, Aleman J, Mollazadeh-Moghaddam K, Bakht SM, Yang J, Jia W, Dell’Erba V, Assawes P, Shin SR, Dokmeci MR, Oklu R, Khademhosseini A. Ann Biomed Eng. 2017;45:148.

    Article  CAS  PubMed  Google Scholar 

  15. Groll J, Burdick JA, Cho DW, Derby B, Gelinsky M, Heilshorn SC, Jüngst T, Malda J, Mironov VA, Nakayama K, Ovsianikov A, Sun W, Takeuchi S, Yoo JJ, Woodfield TBF. Biofabrication. 2019;11:013001. https://doi.org/10.1088/1758-5090/aaec52.

    Article  CAS  Google Scholar 

  16. Ashammakhi N, Ahadian S, Xu C, Montazerian H, Ko H, Nasiri R, Barros N, Khademhosseini A. Mater Today Bio. 2019;1:100008.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Duarte Campos DF, Blaeser A, Weber M, Jäkel J, Neuss S, Jahnen-Dechent W, Fischer H. Biofabrication. 2013;5:015003.

    Article  PubMed  CAS  Google Scholar 

  18. Duarte Campos DF, Blaeser A, Buellesbach K, Sen KS, Xun W, Tillmann W, Fischer H. Adv Healthc Mater. 2016;5:1336.

    Article  CAS  PubMed  Google Scholar 

  19. Blaeser A, Duarte Campos DF, Puster U, Richtering W, Stevens MM, Fischer H. Adv Healthc Mater. 2016;5:326.

    Article  CAS  PubMed  Google Scholar 

  20. Campos DFD, Philip MA, Gürzing S, Melcher C, Lin YY, Schöneberg J, Blaeser A, Theek B, Fischer H, Betsch M, Print 3D. Addit Manuf. 2019;6:63.

    Google Scholar 

  21. Duarte Campos DF, Blaeser A, Korsten A, Neuss S, Jäkel J, Vogt M, Fischer H. Tissue Eng Part A. 2015;21:740.

    Article  CAS  PubMed  Google Scholar 

  22. Duarte Campos DF, Rohde M, Ross M, Anvari P, Blaeser A, Vogt M, Panfil C, Yam GH, Mehta JS, Fischer H, Walter P, Fuest M, Biomed J. Mater Res Part A. 2019;107:1945.

    Article  CAS  Google Scholar 

  23. Duarte Campos DF, Zhang S, Kreimendahl F, Köpf M, Fischer H, Vogt M, Blaeser A, Apel C, Esteves-Oliveira M. Connect Tissue Res. 2019;61:205.

    Article  PubMed  CAS  Google Scholar 

  24. Campos DFD, Marquez AB, O’seanain C, Fischer H, Blaeser A, Vogt M, Corallo D, Aveic S. Cancers (Basel). 2019;11:2.

    Article  CAS  Google Scholar 

  25. Morris VB, Nimbalkar S, Younesi M, McClellan P, Akkus O. Ann Biomed Eng. 2017;45:286.

    Article  PubMed  Google Scholar 

  26. Hockaday LA, Kang KH, Colangelo NW, Cheung PYC, Duan B, Malone E, Wu J, Girardi LN, Bonassar LJ, Lipson H, Chu CC, Butcher JT. Biofabrication. 2012;4:035005.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Wang H, Heilshorn SC. Adv Mater. 2015;27:3717–36.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Blaeser A, Duarte Campos DF, Fischer H. Curr Opin Biomed Eng. 2017;2:58.

    Article  Google Scholar 

  29. Melchels FPW, Feijen J, Grijpma DW. Biomaterials. 2010;31:6121.

    Article  CAS  PubMed  Google Scholar 

  30. Ma X, Qu X, Zhu W, Li Y-S, Yuan S, Zhang H, Liu J, Wang P, Lai CSE, Zanella F, Feng G-S, Sheikh F, Chien S, Chen S. Proc Natl Acad Sci. 2016;113:2206.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Sun C, Fang N, Wu DM, Zhang X. Sensors Actuators A Phys. 2005;121:113.

    Article  CAS  Google Scholar 

  32. Fouassier J, Allonas X, Burget D. Prog Org Coat. 2003;47:16.

    Article  CAS  Google Scholar 

  33. Fedorovich NE, Oudshoorn MH, van Geemen D, Hennink WE, Alblas J, Dhert WJA. Biomaterials. 2009;30:344.

    Article  CAS  PubMed  Google Scholar 

  34. Han D, Yang C, Fang NX, Lee H. Addit Manuf. 2019;27:606.

    CAS  Google Scholar 

  35. Pati F, Jang J, Lee JW, Cho DW. Extrusion bioprinting. In: Essentials of 3D biofabrication and translation. Cambridge: Academic Press; 2015. p. 123–52.

    Chapter  Google Scholar 

  36. Ozbolat IT, Hospodiuk M. Biomaterials. 2016;76:321.

    Article  CAS  PubMed  Google Scholar 

  37. Cohen DL, Malone E, Lipson HOD, Bonassar LJ. Tissue Eng. 2006;12:1325.

    Article  CAS  PubMed  Google Scholar 

  38. Kolesky DB, Truby RL, Gladman AS, Busbee TA, Homan KA, Lewis JA. Adv Mater. 2014;26:1.

    Article  Google Scholar 

  39. Kolesky DB, Homan KA, Skylar-scott MA, Lewis JA. PNAS. 2016;113:3179.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Chang CC, Boland ED, Williams SK, Hoying JB. J Biomed Mater Res B Appl Biomater. 2011;98:160.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  41. Murphy SV, Atala A. Nat Biotechnol. 2014;32:773.

    Article  CAS  PubMed  Google Scholar 

  42. Markstedt K, Mantas A, Tournier I, Martínez Ávila H, Hägg D, Gatenholm P. Biomacromolecules. 2015;16:1489.

    Article  CAS  PubMed  Google Scholar 

  43. Colosi C, Shin SR, Manoharan V, Massa S, Costantini M, Barbetta A, Dokmeci MR, Dentini M, Khademhosseini A. Adv Mater. 2016;28:677.

    Article  CAS  PubMed  Google Scholar 

  44. Akkineni AR, Ahlfeld T, Lode A, Gelinsky M. Biofabrication. 2016;8:45001.

    Article  CAS  Google Scholar 

  45. Serra P, Piqué A. Adv Mater Technol. 2019;4:1.

    Article  CAS  Google Scholar 

  46. Gruene M, Unger C, Koch L, Deiwick A, Chichkov B. Biomed Eng Online. 2011;10:1.

    Article  Google Scholar 

  47. Guillotin B, Souquet A, Catros S, Duocastella M, Pippenger B, Bellance S, Bareille R, Rémy M, Bordenave L, Amédée J, Guillemot F. Biomaterials. 2010;31:7250.

    Article  CAS  PubMed  Google Scholar 

  48. Koch L, Kuhn S, Sorg H, Gruene M, Schlie S, Gaebel R, Polchow B, Reimers K, Stoelting S, Ma N, Vogt PM, Steinhoff G, Chichkov B. Tissue Eng Part C Methods. 2010;16:847.

    Article  CAS  PubMed  Google Scholar 

  49. Schiele NR, Corr DT, Huang Y, Raof NA, Xie Y, Chrisey DB. Biofabrication. 2010;2:032001.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  50. Tasoglu S, Demirci U. Trends Biotechnol. 2013;31:10.

    Article  CAS  PubMed  Google Scholar 

  51. Xiong R, Zhang Z, Chai W, Huang Y, Chrisey DB. Biofabrication. 2015;7:45011.

    Article  Google Scholar 

  52. Tseng F, Kim C, Ho C. J Microelectromech Syst. 2002;11:427.

    Article  Google Scholar 

  53. Laser DJ, Santiago JG. J Micromech Microeng. 2004;14:R35.

    Article  Google Scholar 

  54. Cui X, Boland T. Biomaterials. 2009;30:6221.

    Article  CAS  PubMed  Google Scholar 

  55. Sen AK, Darabi J. J Micromech Microeng. 2007;17:1420.

    Article  CAS  Google Scholar 

  56. Chen P-H, Chen W-C, Chang S-H. Int J Mech Sci. 1997;39:683.

    Article  Google Scholar 

  57. Xu T, Jin J, Gregory C, Hickman JJJJ, Boland T. Biomaterials. 2005;26:93.

    Article  PubMed  CAS  Google Scholar 

  58. Kim JD, Choi JS, Kim BS, Chan Choi Y, Cho YW. Polymer (Guildf). 2010;51:2147.

    Article  CAS  Google Scholar 

  59. Lorber B, Hsiao W-K, Hutchings IM, Martin KR. Biofabrication. 2014;6:015001.

    Article  CAS  PubMed  Google Scholar 

  60. Wijshoff H. Phys Rep. 2010;491:77.

    Article  CAS  Google Scholar 

  61. De Maria C, Ferrari L, Montemurro F, Vozzi F, Guerrazzi I, Boland T, Vozzi G. Procedia Eng. 2015;110:98.

    Article  CAS  Google Scholar 

  62. Matai I, Kaur G, Seyedsalehi A, McClinton A, Laurencin CT. Biomaterials. 2020;226:119536.

    Article  CAS  PubMed  Google Scholar 

  63. Fritz Gyger AG. Produktbroschüre Mikroventile SMLD. 2010. www.fluidics.ch. Accessed 04 Aug 2015.

  64. Faulkner-jones A, Fyfe C, Cornelissen D, Gardner J, King J. Biofabrication. 2015;7:44102.

    Article  Google Scholar 

  65. Gudapati H, Dey M, Ozbolat I. Biomaterials. 2016;102:20.

    Article  CAS  PubMed  Google Scholar 

  66. Demirci U, Montesano G. Lab Chip. 2007;7:1139.

    Article  CAS  PubMed  Google Scholar 

  67. Ellson R, Mutz M, Browning B, Leejr L, Miller M, Papen R. J Assoc Lab Autom. 2003;8:29.

    Article  Google Scholar 

  68. Fang Y, Frampton JP, Raghavan S, Sabahi-Kaviani R, Luker G, Deng CX, Takayama S. Tissue Eng Part C Methods. 2012;18:647.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  69. Timmins NE, Dietmair S, Nielsen LK. Angiogenesis. 2004;7:97.

    Article  PubMed  Google Scholar 

  70. Grimes DR, Kelly C, Bloch K, Partridge M. J R Soc Interf. 2014;11:20131124.

    Article  Google Scholar 

  71. Bhise NS, Manoharan V, Massa S, Tamayol A, Ghaderi M. Biofabrication. 2016;8:14101.

    Article  CAS  Google Scholar 

  72. Kunz-Schughart LA, Freyer JP, Hofstaedter F, Ebner R. J Biomol Screen. 2004;9:273.

    Article  CAS  PubMed  Google Scholar 

  73. Lin R-Z, Lin R-Z, Chang H-Y. Biotechnol J. 2008;3:1172.

    Article  CAS  PubMed  Google Scholar 

  74. Jakab K, Norotte C, Marga F, Murphy K, Vunjak-Novakovic G, Forgacs G. Biofabrication. 2010;2:022001.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  75. Norotte C, Marga FS, Niklason LE, Forgacs G. Biomaterials. 2009;30:5910.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  76. Arai K, Murata D, Verissimo AR, Mukae Y, Itoh M, Nakamura A, Morita S, Nakayama K. PLoS One. 2018;13:1.

    Google Scholar 

  77. Yu Y, Moncal KK, Li J, Peng W, Rivero I, Martin JA, Ozbolat IT. Sci Rep. 2016;6:28714.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. Ong CS, Yesantharao P, Huang CY, Mattson G, Boktor J, Fukunishi T, Zhang H, Hibino N. Pediatr Res. 2018;83:223.

    Article  CAS  PubMed  Google Scholar 

  79. Chimene D, Kaunas R, Gaharwar AK. Adv Mater. 2019;1902026:1.

    Google Scholar 

  80. Chen YC, Lin RZ, Qi H, Yang Y, Bae H, Melero-Martin JM, Khademhosseini A. Adv Funct Mater. 2012;22:2027.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  81. Blaeser A, Million N, Duarte Campos DF, Gamrad L, Köpf M, Rehbock C, Nachev M, Sures B, Barcikowski S, Fischer H. Nano Res. 2016;9:3407.

    Article  CAS  Google Scholar 

  82. Mirahmadi F, Tafazzoli-Shadpour M, Shokrgozar MA, Bonakdar S. Mater Sci Eng C Mater Biol Appl. 2013;33:4786.

    Article  CAS  PubMed  Google Scholar 

  83. Kosik-Kozioł A, Costantini M, Bolek T, Szöke K, Barbetta A, Brinchmann J, Świȩszkowski W. Biofabrication. 2017;9:044105. https://doi.org/10.1088/1758-5090/aa90d7.

    Article  CAS  PubMed  Google Scholar 

  84. Kumar BYS, Isloor AM, Kumar GCM, Inamuddin, Asiri AM. Sci Rep. 2019;9:1.

    Article  Google Scholar 

  85. Köpf M, Campos DFD, Blaeser A, Sen KS, Fischer H. Biofabrication. 2016;8:1.

    Article  CAS  Google Scholar 

  86. Kreimendahl F, Köpf M, Thiebes AL, Duarte Campos DF, Blaeser A, Schmitz-Rode T, Apel C, Jockenhoevel S, Fischer H. Tissue Eng Part C Methods. 2017;23:604.

    Article  CAS  PubMed  Google Scholar 

  87. Shim J-H, Kim JY, Park M, Park J, Cho D-W. Biofabrication. 2011;3:034102.

    Article  PubMed  CAS  Google Scholar 

  88. Yoon Y, Kim CH, Lee JE, Yoon J, Lee NK, Kim TH, Park SH. Biofabrication. 2019;11:025015. https://doi.org/10.1088/1758-5090/ab08c2.

    Article  CAS  PubMed  Google Scholar 

  89. de Ruijter M, Ribeiro A, Dokter I, Castilho M, Malda J. Adv Healthc Mater. 2019;8:e1800418. https://doi.org/10.1002/adhm.201800418.

    Article  CAS  PubMed  Google Scholar 

  90. Kang H-W, Lee SJ, Ko IK, Kengla C, Yoo JJ, Atala A. Nat Biotechnol. 2016;34:312.

    Article  CAS  PubMed  Google Scholar 

  91. Wu W, DeConinck A, a Lewis J. Adv Mater. 2011;23:H178.

    Article  CAS  PubMed  Google Scholar 

  92. Tabriz AG, Hermida MA, Leslie NR, Shu W. Biofabrication. 2015;7:045012.

    Article  PubMed  Google Scholar 

  93. Boland T, Xu T, Damon B, Cui X. Biotechnol J. 2006;1:910.

    Article  CAS  PubMed  Google Scholar 

  94. Radisic M, Park H, Chen F, Salazar-Lazzaro JE, Wang Y, Dennis R, Langer R, Freed LE, Vunjak-Novakovic G. Tissue Eng. 2006;12:2077.

    Article  CAS  PubMed  Google Scholar 

  95. Lowe KC. Tissue Eng. 2003;9:389.

    Article  CAS  PubMed  Google Scholar 

  96. Blaeser A, Duarte Campos DF, Weber M, Neuss S, Theek B, Fischer H, Jahnen-Dechent W. Biores Open Access. 2013;2:374.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  97. Hinton TJ, Jallerat Q, Palchesko RN, Park JH, Grodzicki MS, Shue HJ, Ramadan MH, Hudson AR, Feinberg AW. Sci Adv. 2015;1:e1500758. https://doi.org/10.1126/sciadv.1500758.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  98. Lee A, Hudson AR, Shiwarski DJ, Tashman JW, Hinton TJ, Yerneni S, Bliley JM, Campbell PG, Feinberg AW. Science. 2019;365:482.

    Article  CAS  PubMed  Google Scholar 

  99. Blaeser A, Duarte Campos DF, Köpf M, Weber M, Fischer H. RSC Adv. 2014;4:46460.

    Article  CAS  Google Scholar 

  100. Jia W, Gungor-Ozkerim PS, Zhang YS, Yue K, Zhu K, Liu W, Pi Q, Byambaa B, Dokmeci MR, Shin SR, Khademhosseini A. Biomaterials. 2016;106:58.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  101. Vacanti CA, Vacanti JP. Orthop Clin North Am. 2000;31:351.

    Article  CAS  PubMed  Google Scholar 

  102. Daly AC, Cunniffe GM, Sathy BN, Jeon O, Alsberg E, Kelly DJ. Adv Healthc Mater. 2016;5:2353.

    Article  CAS  PubMed  Google Scholar 

  103. Daly AC, Freeman FE, Gonzalez-Fernandez T, Critchley SE, Nulty J, Kelly DJ. Adv Healthc Mater. 2017;6:1.

    Article  CAS  Google Scholar 

  104. Singh YP, Bandyopadhyay A, Mandal BB. ACS Appl Mater Interfaces. 2019;11:33684.

    Article  CAS  PubMed  Google Scholar 

  105. Rathan S, Dejob L, Schipani R, Haffner B, Möbius ME, Kelly DJ. Adv Healthc Mater. 2019;8:1.

    Article  CAS  Google Scholar 

  106. Piard C, Baker H, Kamalitdinov T, Fisher J. Biofabrication. 2019;11:025013. https://doi.org/10.1088/1758-5090/ab078a.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  107. Merceron TK, Burt M, Seol Y-J, Kang H-W, Lee SJ, Yoo JJ, Atala A. Biofabrication. 2015;7:035003.

    Article  PubMed  CAS  Google Scholar 

  108. Michael S, Sorg H, Peck C-T, Koch L, Deiwick A, Chichkov B, Vogt PM, Reimers K. PLoS One. 2013;8:e57741.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  109. Yurie H, Ikeguchi R, Aoyama T, Kaizawa Y, Tajino J, Ito A, Ohta S, Oda H, Takeuchi H, Akieda S, Tsuji M, Nakayama K, Matsuda S. PLoS One. 2017;12:1.

    Article  CAS  Google Scholar 

  110. Ke D, Yi H, Est-Witte S, George S, Kengla C, Atala A. Biofabrication. 2019;12:015022. https://doi.org/10.1088/1758-5090/ab5354.

    Article  CAS  PubMed  Google Scholar 

  111. Marchioli G, van Gurp L, van Krieken PP, Stamatialis D, Engelse M, van Blitterswijk CA, Karperien MBJ, de Koning E, Alblas J, Moroni L, van Apeldoorn AA. Biofabrication. 2015;7:025009.

    Article  CAS  PubMed  Google Scholar 

  112. Marchioli G, Di Luca A, De Koning E, Engelse M, Van Blitterswijk CA, Karperien M, Van Apeldoorn AA. Adv Healthc Mater. 2016;5:1606.

    Article  CAS  PubMed  Google Scholar 

  113. Cui H, Zhu W, Nowicki M, Zhou X, Khademhosseini A, Zhang LG. Adv Healthc Mater. 2016;5:395.

    Article  CAS  Google Scholar 

  114. Yang F-Y, Yu M-X, Zhou Q, Chen W-L, Gao P, Huang Z. Cell Transplant. 2012;21:1805.

    Article  PubMed  Google Scholar 

  115. Abbadessa A, Mouser VHM, Blokzijl MM, Gawlitta D, Dhert WJA, Hennink WE, Malda J, Vermonden T. Biomacromolecules. 2016;17:2137.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  116. Costantini M, Idaszek J, Szöke K, Jaroszewicz J, Dentini M, Barbetta A, Brinchmann JE, Święszkowski W. Biofabrication. 2016;8:035002.

    Article  PubMed  CAS  Google Scholar 

  117. Gao B, Yang Q, Zhao X, Jin G, Ma Y, Xu F. Trends Biotechnol. 2016;34:746.

    Article  CAS  PubMed  Google Scholar 

  118. Hsieh FY, Lin HH, Hsu SH. Biomaterials. 2015;71:48.

    Article  CAS  PubMed  Google Scholar 

  119. Shim J-H, Jang K-M, Hahn SK, Park JY, Jung H, Oh K, Park KM, Yeom J, Park SH, Kim SW, Wang JH, Kim K, Cho D-W. Biofabrication. 2016;8:014102.

    Article  PubMed  CAS  Google Scholar 

  120. Huang S, Yao B, Xie J, Fu X. Acta Biomater. 2016;32:170.

    Article  CAS  PubMed  Google Scholar 

  121. Huang Y, Zhang XF, Gao G, Yonezawa T, Cui X. Biotechnol J. 2017;12:1600734. https://doi.org/10.1002/biot.201600734.

  122. Martin I, Wendt D, Heberer M. Trends Biotechnol. 2004;22:80.

    Article  CAS  PubMed  Google Scholar 

  123. Chen HC, Hu YC. Biotechnol Lett. 2006;28:1415.

    Article  CAS  PubMed  Google Scholar 

  124. Stevens MM, Marini RP, Schaefer D, Aronson J, Langer R, Shastri VP. Proc Natl Acad Sci U S A. 2005;102:11450.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  125. Kim J, Ma T. Tissue Eng Part A. 2012;18:120719071626004.

    Google Scholar 

  126. Heher P, Maleiner B, Prüller J, Teuschl AH, Kollmitzer J, Monforte X, Wolbank S, Redl H, Rünzler D, Fuchs C. Acta Biomater. 2015;24:251.

    Article  CAS  PubMed  Google Scholar 

  127. Moreira R, Velz T, Alves N, Gesche VN, Malischewski A, Schmitz-Rode T, Frese J, Jockenhoevel S, Mela P. Tissue Eng Part C Methods. 2015;21:530.

    Article  CAS  PubMed  Google Scholar 

  128. Radisic M, Marsano A, Maidhof R, Wang Y, Vunjak-Novakovic G. Nat Protoc. 2008;3:719.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  129. Lee V, Singh G, Trasatti J, Bjornsson C, Xu X, Tran T, Yoo S, Dai G, Karande P. Tissue Eng Part C. 2014;20:473.

    Article  CAS  Google Scholar 

  130. Jeon H, Kang K, Park SA, Kim WD, Paik SS, Lee S-H, Jeong J, Choi D. Gut Liver. 2017;11:121.

    Article  PubMed  Google Scholar 

  131. Miri AK, Mostafavi E, Khorsandi D, Hu SK, Malpica M, Khademhosseini A. Biofabrication. 2019;11:042002. https://doi.org/10.1088/1758-5090/ab2798.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  132. Schöneberg J, De Lorenzi F, Theek B, Blaeser A, Rommel D, Kuehne AJC, Kießling F, Fischer H. Sci Rep. 2018;8:1.

    Article  CAS  Google Scholar 

  133. Campos DFD, Lindsay CD, Roth JG, Lesavage BL, Seymour AJ, Krajina BA, Ribeiro R, Costa PF, Blaeser A, Heilshorn SC. Front Bioeng Biotechnol. 2020;8:1.

    Article  Google Scholar 

  134. Zhang YS, Arneri A, Bersini S, Shin S-R, Zhu K, Goli-Malekabadi Z, Aleman J, Colosi C, Busignani F, Dell’Erba V, Bishop C, Shupe T, Demarchi D, Moretti M, Rasponi M, Dokmeci MR, Atala A, Khademhosseini A. Biomaterials. 2016;110:45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  135. Homan KA, Kolesky DB, Skylar-Scott MA, Herrmann J, Obuobi H, Moisan A, Lewis JA. Sci Rep. 2016;6:34845.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  136. Horváth L, Umehara Y, Jud C, Blank F, Petri-Fink A, Rothen-Rutishauser B. Sci Rep. 2015;5:7974.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  137. Dai X, Ma C, Lan Q, Xu T. Biofabrication. 2016;8:045005.

    Article  PubMed  CAS  Google Scholar 

  138. Zhou X, Zhu W, Nowicki M, Miao S, Cui H, Holmes B, Glazer RI, Zhang LG. ACS Appl Mater Interfaces. 2016;8:30017.

    Article  CAS  PubMed  Google Scholar 

  139. Zhang B, Montgomery M, Chamberlain MD, Ogawa S, Korolj A, Pahnke A, Wells LA, Masse S, Kim J, Reis L, Momen A, Nunes SS, Wheeler AR, Nanthakumar K, Keller G, Sefton MV, Radisic M. Nat Mater. 2016;15:669.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  140. Lind JU, Busbee TA, Valentine AD, Pasqualini FS, Yuan H, Yadid M, Park S, Kotikian A, Nesmith AP, Campbell PH, Vlassak JJ, Lewis JA, Parker KK. Nat Mater. 2017;16:303. https://doi.org/10.1038/NMAT4782.

    Article  CAS  PubMed  Google Scholar 

  141. Skardal A, Devarasetty M, Kang H-W, Mead I, Bishop C, Shupe T, Lee SJ, Jackson J, Yoo J, Soker S, Atala A. Acta Biomater. 2015;25:24.

    Article  CAS  PubMed  Google Scholar 

  142. Ma X, Qu X, Zhu W, Li Y, Yuan S, Zhang H, Liu J, Wang P. Proc Natl Acad Sci. 2016;113:2206.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  143. Lee H, Cho D-W. Lab Chip. 2016;16:2618.

    Article  CAS  PubMed  Google Scholar 

  144. Nguyen DG, Funk J, Robbins JB, Crogan-Grundy C, Presnell SC, Singer T, Roth AB. PLoS One. 2016;11:1.

    Google Scholar 

  145. Gu Q, Tomaskovic-Crook E, Lozano R, Chen Y, Kapsa RM, Zhou Q, Wallace GG, Crook JM. Adv Healthc Mater. 2016;5:1429.

    Article  CAS  PubMed  Google Scholar 

  146. Hou X, Liu S, Wang M, Wiraja C, Huang W, Chan P, Tan T, Xu C. SLAS Technol Transl Life Sci Innov. 2017;22:447. https://doi.org/10.1177/2211068216655753.

    Article  Google Scholar 

  147. Zhang YS, Davoudi F, Walch PDK, Manbachi A, Luo X, Dell’Erba V, Miri A, Albadawi H, Arneri A, Li X, Wang X, Dokmeci M, Khademhosseini A, Oklu R. Lab Chip. 2016;16:4097.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  148. Paul SM, Mytelka DS, Dunwiddie CT, Persinger CC, Munos BH, Lindborg SR, Schacht AL. Nat Rev Drug Discov. 2010;9:203.

    Article  CAS  PubMed  Google Scholar 

  149. Zhang YS, Arneri A, Bersini S, Shin S-R, Zhu K, Malekabadi ZG, Aleman J, Colosi C, Busignani F, Dell’Erba V. Biomaterials. 2016;110:45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Materials Science & Engineering, Stanford University, Stanford, CA, USA

    Daniela F. Duarte Campos

  2. AME – Institute of Applied Medical Engineering, RWTH Aachen University, Aachen, Germany

    Daniela F. Duarte Campos

  3. BioMedical Printing Technology, TU Darmstadt, Darmstadt, Germany

    Andreas Blaeser

  4. Centre for Synthetic Biology, TU Darmstadt, Darmstadt, Germany

    Andreas Blaeser

Authors
  1. Daniela F. Duarte Campos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andreas Blaeser
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andreas Blaeser .

Editor information

Editors and Affiliations

  1. Institute of Cell and Tissue Culture Technologies, University of Natural Resources and Life Sciences, Vienna, Austria

    Cornelia Kasper

  2. Institute of Cell and Tissue Culture Technologies, University of Natural Resources and Life Sciences, Vienna, Austria

    Dominik Egger

  3. Institute of Technical Chemistry, Leibniz University of Hannover, Hannover, Germany

    Antonina Lavrentieva

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Nature Switzerland AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Duarte Campos, D.F., Blaeser, A. (2021). 3D-Bioprinting. In: Kasper, C., Egger, D., Lavrentieva, A. (eds) Basic Concepts on 3D Cell Culture . Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-66749-8_9

Download citation

Publish with us

Policies and ethics

Search

Navigation