Abstract
The advent of drug discovery and advancement in developing new class of antimicrobial drugs has affected the microbial population. However, the microbial population has been very smart in developing themselves in order to overcome the onslaught of antimicrobial drugs and have evolved, rendering most of the commonly used antimicrobial drugs less effective. The world is looking at a problem where the infectious strains of microbes are emerging successful in the battle of drugs versus microbes. Researchers are working on different ways to overcome antimicrobial resistance. Gene editing using clustered regularly interspaced short palindromic repeats and its associated proteins (CRISPR-Cas) technique has emerged as one of the potential techniques to overcome antimicrobial resistance in microbes.
CRISPR-Cas machinery is an adaptive immune system found in bacteria and archaea. Using this machinery, the organisms eliminate the foreign invading genetic material. Literature reports suggest the potential of CRISPR-Cas system in removing antibiotic resistant genes in various strains of bacteria. The chapter enlists some of the prominent studies carried out to mitigate antimicrobial resistance using CRISPR-Cas system. The different strategies to deliver CRISPR-Cas system in microbes has been discussed in the chapter. The chapter also outlines the challenges associated with this novel technique against the emerging antimicrobial resistance.
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Abbreviations
- CRISPR :
-
Clustered Regularly Interspaced Short Palindromic Repeats
- Cas :
-
CRISPR-associated proteins
- FDA:
-
Food and Drug Administration
- crRNA:
-
CRISPR RNA
- Acrs :
-
anti-CRISPRs
- pre-crRNA :
-
Precursor CRISPR RNA
- tract-RNA:
-
Trans activating RNA
- dsRNA:
-
Double strand RNA
- PAM :
-
Protospacer adjacent motif
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Sarma, A.P., Jain, C., Solanki, M., Ghangal, R., Patnaik, S. (2020). Role of Gene Editing Tool CRISPR-Cas in the Management of Antimicrobial Resistance. In: Panwar, H., Sharma, C., Lichtfouse, E. (eds) Sustainable Agriculture Reviews 46. Sustainable Agriculture Reviews, vol 46. Springer, Cham. https://doi.org/10.1007/978-3-030-53024-2_6
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