Silkworm: A Unique Creature for Natural Products

  • R. Venkatesh Kumar
  • Devika Srivastava


Insects are an exemplary source of natural products. However, still they are considered a marginalized source of natural products, although their number is sizable, and have great pharmacological potential of natural products when compared to another group of animals. Among beneficial insects, silkworms and their by-products have emerged as the most valuable creatures due to their incredible medicinal properties and other usages. Apart from production of silk, silkworms are being utilized in several ways, such as a source of nutrients for human consumption, as cattle feed, as an antipollutant, used in the manufacturing of vaccines, and as a bioreactors for the production of recombinant proteins. Further, silkworm pupae, pupal powder, and pupal oil have shown spectacular biological activities such as neuroprotective, antidiabetic, hypolipidemic, antioxidant, anticancer, and antibacterial. This chapter summarizes the usages of silkworms and their by-products with their chemical constituents, proven biological activities, biomedical and pharmaceutical application of silkworm, and their by-products.


Silkworms Silkworm excreta Pupae Papal oil Biological activities 


  1. Ahn MY, Shim SH, Jeong HK, Ryu KS (2008) Purification of a dimethyladenosine compound from silkworm pupae as a vasorelaxation substance. J Ethnopharmacol 117:115–122CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bodenheimer FS (1951) Insects as human food. W. Junk, The Hague, p 1951CrossRefGoogle Scholar
  3. Buhroo ZI, Bhat MA, Kamili AS, Ganai NA, Bali GK, Khan IL, Aziz A (2018) Trends in development and utilization of sericulture resources for diversification and value addition. J Entomol Zool Stud 6(4):601–615Google Scholar
  4. Chamberland JP, Moon HS (2014) Down-regulation of malignant potential by alpha linolenic acid in human and mouse colon cancer cells. Fam CancerGoogle Scholar
  5. Chukiatsiri S, Hangtrakul W (2018) Biological activities of protein extracts from silkworm pupae against non-communicable disease. In: The 6th international conference on biochemistry and molecular biology, 1–11Google Scholar
  6. Dai J, Shen J, Pan W, Shen S, Undurti ND (2013) Effects of polyunsaturated fatty acids on the growth of gastric cancer cells in vitro. Lipid Heal Dis 12:71CrossRefGoogle Scholar
  7. Datta RK (1994) Silkworms to produce human vaccine. Ind Silk 33:33–35Google Scholar
  8. Deshpande R, Mansara P, Suryavanshi S, Ghanekar RK (2013) Alpha-linolenic acid regulates the growth of breast and cervical cancer cell lines through regulation of NO release and induction of lipid peroxidation. J Mol Biochem 2:6–17Google Scholar
  9. Dev P, Ramappa VK, Gopal R, Sangeeta (2017) Analysis of the chemical composition of mulberry silkworm pupal oil with Fourier transforms infrared spectroscopy (FTIR), gas chromatography spectrometry (GCMS) and its antimicrobial property. Asian J Agri Res 11(4):108–115Google Scholar
  10. Dutta RN, Majumdar SK, Kar R, Bajpai AK (2010) Incense stick from silkworm excreta. Indian Silk, p 13Google Scholar
  11. Gallo MBC, Sarachine MJ (2009) Biological activities of lupeol. Int J Biomed Pharm Sci 3(Special Issue 1):46–66Google Scholar
  12. Gavia MH, Couto RC, Oyama LM et al (2003) Diets rich in polyunsaturated fatty acids effects on hepatic metabolism in rats. Nutri 19:144–149CrossRefGoogle Scholar
  13. Gui Z, Zhuang D (2000) Study on the silkworm powder and its physiological functions. China Sericult 2(5):53–54Google Scholar
  14. Imai K, Sugiura K, Komiya T, Yamashita O (1996) Isolation and partial structure of a unique lipophilic peptide, VAPpeptide from the heads of male silkworm moths. Biosci Biotech Biochem 60:355–357CrossRefGoogle Scholar
  15. Iyengar, MNS (2007) Indian Silk, p 15Google Scholar
  16. Iyengar MNS (2010) Development of special purpose silks. Indian Silk, p 18Google Scholar
  17. Ji SD, Kim NS, Kweon HY, Choi BH, Kim KY, Koh YH (2016) Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons. Int J Indust Entomol 33(1):6–14CrossRefGoogle Scholar
  18. Jian C, Xiang-Fu W, Zhou Z (2006) Expression, purification, and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor. J Biotech 123:236–247Google Scholar
  19. Joy O, Gopinathan KP (1994) Expression of microinjected foreign DNA in silkworm, Bombyx mori. Curr Sci 66(2):145–150Google Scholar
  20. Kawasaki H, Sato H, Suzuki M (1970) Structural proteins in the silkworm egg-shells. Insect Biochem:130–148Google Scholar
  21. Kunz RI, Brancalhao RMC, Fatima LD, Ribeiro C, Maria Raquel MarcalNatali MRM (2016) Silkworm sericin: properties and biomedical applications. Biomed Res Int. 19 pagesGoogle Scholar
  22. Kwon MG, Kim DS, Lee JH, Park SW, Choo YK, Han YS, Kim JS, Hwang KA, Kinarm K, Kisung KO (2012) Isolation and analysis of natural compounds from silkworm pupae and effect of its extracts on alcohol detoxification. Entomolog Res 42(1):55–62CrossRefGoogle Scholar
  23. Lawrence BD (2014) Processing of Bombyx mori silk for biomedical application. In: Silk biomaterials for the tissue engineering and regenerative medicine, pp 78–99CrossRefGoogle Scholar
  24. Liu J, Rajendram R, Zhang L (2010) Chapter 158: Effects of oloeanolic acid and maslinic acid on glucose and lipid metabolism: implications for the beneficial effects of olive oil on health. In: Olives and olive oil in health and disease prevention, pp 1423–1429CrossRefGoogle Scholar
  25. Łochynska M, Frankowski J (2018) The biogas production potential from silkworm waste. Waste Manag 79:564–570CrossRefPubMedPubMedCentralGoogle Scholar
  26. Longvah T, Manghtya K, Qadri SSYH (2012) Eri silkworm: a source of edible oil with a high content of α- linolenic acid and of significant nutritional value. J Sci Food Agric 92:1988–1993CrossRefPubMedPubMedCentralGoogle Scholar
  27. Mahesh DS, Vidharthi BS, Narayanswamy TK, Subbarayappa CT, Muthuraju R, Shrushti P (2015) Bionutritional Science of Silkworm Pupal residue to Mine new ways for utilization. Int J Adv Res Biol Sci 2(9):135–140Google Scholar
  28. Mark Index, 11th Edition, 9506Google Scholar
  29. Matsuda S, Nerome R, Maegawa K, Kotaki A, Sugita S, Kawasaki K, Kuroda K, Yamaguchi R, Takasaki T, Nerome K (2017) Development of a Japanese encephalitis virus-like particle vaccine in silk worms using codon-optimised prM and envelope genes. Heliyon:1–7Google Scholar
  30. Muhammad FM, Ahsan M, Abdul W (2018) Quercetin – a mini review. Mod Concep Dev Agrono:1–5Google Scholar
  31. Nazim N, Buhroo ZI, Mushtaq N, Javid K, Rasool S, Mir GM (2017) Medicinal values of products and by products of sericulture. J Pharma Phytochem 6(5):1388–1392Google Scholar
  32. Nerome K, Kuroda K, Sugita S, Kawasaki K, Iinuma H, Nerome SMR (2015) The usefulness of an influenza Virus-Like Particle (VLP) vaccine produced in silkworm pupae and virosomes and liposomes prepared by chemical means: from Virosome to VLP and the future of vaccines. J Gastrointestinal Diges Syst 5(1):1–7Google Scholar
  33. Olumuyiwa T, Omotoso TO (2015) An evaluation of the nutrients and some anti-nutrients in silkworm, Bombyx mori L. (Bombycidae: Lepidoptera). Jordan J Biol Sci 8(1):45–50CrossRefGoogle Scholar
  34. Pachiappan P, Mohaneaj P, Thangamalar CAA (2016) In vitro evaluation of the antioxidant activity of bioproducts extracted from silkworm pupae. Environ We Int J Sci Tech 11:33–39Google Scholar
  35. Paulino AT, Simionato JI, Garcia JC, Nozaki J (2006) Characterization of chitosan and chitin produced from silkworm chrysalides. Carbohydrate Poly 64:98–103CrossRefGoogle Scholar
  36. Priyadarshini P, Maria Joncy M, Saratha AM (2017) Industrial utilization of silkworm pupae – a review. J Int Acad Res Multidis 5:2320–5083Google Scholar
  37. Rahmasari R, Sumiati, Astuti DA (2014) The effects of silkworm pupae (Bombyx mori) meal to substitute fish meal on production and physical quantity of quail eggs (Cortunixcortunix japonica). Indonesian Trop Anim Agric 39(3):180–187Google Scholar
  38. Rajakumar S, Chikkanna, Bindroo BB (2014) Food and medicinal values in “silkworm” and its host plant “Mulberry”-exploring new horizons. Int J Food Nutr Sci 3(1):124–130Google Scholar
  39. Ramakanth, Raman KVA (1997) Cocoon Pelade for better health. Ind Silk 35:35Google Scholar
  40. Ramappa VK, Dev P, Kumar Y (2017) Silkworm pupal oil: a novel source of omega-3 fatty acid. Ind Silk 7-8:24–25Google Scholar
  41. Rao UP (1994) Chemical composition and nutritional evaluation of spent silk worm pupae. J Agric Food Chem 42:2201–2203CrossRefGoogle Scholar
  42. Rattana S, Katisart T, Sungthong B, Butiman C (2017) Pharmacogn J. Acute and sub-acute toxicities of Thai silkworm powder (Bombyx mori Linn.) from three races in male wistar rats and In vitro antioxidant activities. Pharmacogn J 9(4):541–545Google Scholar
  43. Ryu SP (2014) Silkworm pupae powder ingestion increases fat metabolism in swim-trained rats. J Exerc Nutr Biochem 18(2):141–149CrossRefGoogle Scholar
  44. Ryu KS, Lee HS, Choue RW (1997) An activity of lowering blood-glucose levels according to preparative condition of silkworm powder. Korean J Sericulture Sci:39–79Google Scholar
  45. Simionato JI, Paulino AT, Garcia JC, Nozaki J (2006) Adsoption of Aluminium from waste water by chitin and chitosan produced from silkworm chrysalides. Polym Int 55:1243–1248CrossRefGoogle Scholar
  46. Simionato JI, Villalobos LDG, Bulla MK, Augusto F, Coro G, Garcia JC (2014) Application of chitin and chitosan extracted from silkworm chrysalides in the treatment of textile effluents contaminated with remazoldye. ActaScientiarium 36:693–698Google Scholar
  47. Singh KP, Jayasomu RS (2002) Bombyx mori – A review of its potential as a medicinal insect. Pharmaceutical Biol 40(1):28–32Google Scholar
  48. Soumya M, Harinatha AR, Nageswari G, Venkatappa B (2017) Silkworm (Bombyx mori) and its constituents: A fascinating insect in science and research. J Entomol Zool Stud 5(5):1701–1705Google Scholar
  49. Sravan GK, Das UN (1997) Cytotoxic action of alpha-Linolenic acid and eicosapantanoic acids on myeloma cells in vitro. Prostaglandin Leukot Essent Fatty Acids 56(4):285–293CrossRefGoogle Scholar
  50. Suresh HN, Mahalingam CA, Pallavi (2012) Amount of chitin, chitosan and chitosan based on chitin weight in pure races of multivoltine and bivoltine silkworm pupae Bombyx mori L. Int J Sci Nature 3(1):214–216Google Scholar
  51. Terada S, Nishimura T, Sasaki M, Yamada H, Miki M (2002) Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma. Cytotechnology 40:3–12CrossRefPubMedPubMedCentralGoogle Scholar
  52. Tomotake H, Katagiri M, Yamato M (2010) Silkworm pupae (Bombyx mori) are a new source of high-quality protein and lipid. J Nutr Sci Vitaminol 56:446–448CrossRefPubMedPubMedCentralGoogle Scholar
  53. Tulp M, Bohlin L (2004) Unconventional natural sources for future drug discovery. Drug Discov Today 9:450–458CrossRefPubMedPubMedCentralGoogle Scholar
  54. Uttara B, Singh AV, Zamboni P, Maharajan RT (2009) Oxidative stress and neurodenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 7:65–74CrossRefPubMedPubMedCentralGoogle Scholar
  55. Vijayakumar RS (1996) Sericulture By-products of China. Ind Silk 34:19Google Scholar
  56. Vimolmankang S, Somkhanngeon C, Sukrong S, Mai C (2014) Potential pharmaceutical uses of the isolated Compounds from silkworm excreta. J Sci 41(1):97–104Google Scholar
  57. Wattanathorn J, Muchimapura S, Boosel A, Kongpa S, Kaewrueng W, Un TT, Wannanon P, Thukhamee W (2012) Silkworm pupae protect against Alzheimer’s disease. Am J Agri Biol Sci 7(3):330–336CrossRefGoogle Scholar
  58. Wilt T, Ishani A, MacDonald R, Stark G, Mulrow C, Lau J (2000) Beta-sitostreols for benign prostatic hyperplasia. Cochrane Lib (2):CD001043Google Scholar
  59. Xia B, Li Z, Ding Y (1989) Properties of the ultraviolet spectrum of domestic silkworm chorionins. CanyeKexue 15:45–48Google Scholar
  60. Yellamma K (2018) Metabolic turnover of carbohydrates during pupal-adult transition stage in the silk worm, Bombyx mori. Int J Dev Res 8(1):18164–18170Google Scholar
  61. Young IS, Woodside (2001) Antioxidants in health and disease. J Clin Pathol 54:176–186CrossRefPubMedPubMedCentralGoogle Scholar
  62. Zhang H, Lin Y, Shen G, Tan X, Lei C, Long W, Liu H, Zhang Y, Xu Y, Wu J, Gu J (2017) Pigmentary analysis of eggs of the silkworm Bombyx mori. J Insect Physiol 101:142–150Google Scholar
  63. Zhao HP, Feng XQ, Cui WZ, Zou FZ (2007) Mechanical properties of silkworm cocoon pelades. Eng Frac Mech 74:1953–1962CrossRefGoogle Scholar
  64. Zheng T, Su S, Dai X, Zhang L, Duan JA, Yang ZO (2018) Metabolomic analysis of biochemical changes in the serum and urine of freund’s adjuvant-induced arthritis in rats after treatment with silkworm excrement. Molecules 23:1490CrossRefGoogle Scholar
  65. Zou J, Han D (2006) Proximate, amino acid and mineral composition of pupae of the silkworm Antheraea pernyi. J Food Comp Anal:850–853Google Scholar

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Authors and Affiliations

  • R. Venkatesh Kumar
    • 1
  • Devika Srivastava
    • 1
  1. 1.Department of ZoologyBabasaheb Bhimrao Ambedkar UniversityLucknowIndia

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