Tissue Culture-Mediated Biotechnological Advancements in Genus Brassica

  • Monisha Mitra
  • Saikat GantaitEmail author


Brassica is documented as a distinguished genus, comprising a wide array of species, belonging to the family Brassicaceae that are majorly consumed as vegetables and processed for oil and condiments. Conventional breeding approaches are laborious; in addition, higher environmental influence interferes with the yield and quality of crop; hence, biotechnological intervention becomes a major requirement. Till date, a lot of studies have been carried out on biotechnological advancements of genus Brassica. Among those, tissue culture-mediated approaches, such as development of protocols for in vitro mass propagation, somaclonal variation, and secondary metabolites production, are highlighted in this chapter. Additionally, genetic transformation and protoplast culture, the other two major tissue culture-based methods, which were substantially used for genetic improvement of Brassica, have also been discussed.


Agrobacterium Callus Genetic transformation Micropropagation Protoplast culture Somatic embryogenesis 



2,4-dichlorophenoxy acetic acid


Gamborg’s medium






Indole-3-acetic acid


Indole-3-butyric acid




Murashige and Skoog medium


α-Naphthalene acetic acid


Plant growth regulator


Somatic embryo


N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea or Thidiazuron


4-hydroxy-3-methyl-terms-2-butenyl aminopurine



The authors acknowledge the e-library assistance from the Central Library, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, West Bengal, India. Further, they are thankful to the anonymous reviewers and the editor of this chapter for their critical comments and suggestions on the manuscript.


  1. Akin-Idowu PE, Ibitoye DO, Ademoyegun OT (2009) Tissue culture as a plant production technique for horticultural crops. Afr J Biotechnol 8:3782–3788Google Scholar
  2. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218CrossRefGoogle Scholar
  3. Baillie AMR, Epp DJ, Hucheson D, Keller WA (1992) In vitro culture of isolated microspores and regeneration of plants in Brassica campestris. Plant Cell Rep 11:234–237PubMedGoogle Scholar
  4. Basak H, Biswas BK, Azad MAK, Arifuzzaman M, Sharmeen F (2012) Micropropagation of mustard (Brassica spp.) from leaf explants. Thai J Agric Sci 45:75–81Google Scholar
  5. Beranek M, Bechyně M, Klíma M (2007) Protoplast isolation and fusion between Brassica carinata Braun. and Brassica rapa L. Agric Trop Subtrop 40:1–6Google Scholar
  6. Berthomieu P, Jouanin L (1992) Transformation of rapid cycling cabbage (Brassica oleracea var. capitata) with Agrobacterium rhizogenes. Plant Cell Rep 11:334–338CrossRefGoogle Scholar
  7. Bhala PL, Singh MB (2008) Agrobacterium mediated transformation of Brassica napus and Brassica oleracea. Nat Protoc 3:181–189CrossRefGoogle Scholar
  8. Bhattacharya RC, Viswakarma N, Bhat SR, Kirti PB, Chopra VL (2002) Development of insect resistant transgenic cabbage plants expressing a synthetic cryIA (b) gene from Bacillus thuringiensis. Curr Sci 83:146–150Google Scholar
  9. Bhattacharya RC, Maheswari M, Dineshkumar V, Kirti PB, Bhat SR, Chopra VL (2004) Transformation of Brassica oleracea var. capitata with bacterial betA gene enhances tolerance to salt stress. Sci Hortic 100:215–227CrossRefGoogle Scholar
  10. Blackshaw RE, Kanashiro D, Moloney MM, Crosby WL (1994) Growth, yield and quality of canola expressing resistance to acetolactate synthase inhibiting herbicides. Can J Plant Sci 74:745–751CrossRefGoogle Scholar
  11. Brewer EP, Saunders JA, Angle JS, Chaney RL, McIntosh MS (1999) Somatic hybridization between the zinc accumulator Thlaspi caerulescens and Brassica napus. Theor Appl Genet 99:761–771CrossRefGoogle Scholar
  12. Burbulis N, Kuprienė R, Blinstrubienė A (2008) Callus induction and plant regeneration from somatic tissue in spring rapeseed (Brassica napus L). Biologija 54:258–263Google Scholar
  13. Burnett L, Arnoldo M, Yarrow S, Huang B (1994) Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tiss Org Cult 37:253–256Google Scholar
  14. Cao J, Earle ED (2003) Transgene expression in broccoli (Brassica oleracea var. italica) clones propagated in vitro via leaf explants. Plant Cell Rep 21:789–796CrossRefGoogle Scholar
  15. Cao J, Tang JD, Strizhov N, Shelton AM, Earle ED (1999) Transgenic broccoli with high levels of Bacillus thuringiensis Cry1C protein control diamondback moth larvae resistant to Cry1A or Cry1C. Mol Breed 5:131–141CrossRefGoogle Scholar
  16. Cardi T, Earle ED (1997) Production of new CMS Brassica oleracea by transfer of Anand cytoplasm from B. rapa through protoplast fusion. Theor Appl Genet 94:204–212CrossRefGoogle Scholar
  17. Cardoza V, Stewart CN Jr (2003) Increased Agrobacterium mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl explants. Plant Cell Rep 21:599–604CrossRefGoogle Scholar
  18. Cardoza V, Stewart CN Jr (2004) Brassica biotechnology: progress in cellular and molecular biology. In Vitro Cell Dev Biol Plant 40:542–551CrossRefGoogle Scholar
  19. Chamandosti F, Majd A, Mehrabian S (2006) In vitro plant regeneration from callus of cotyledons in canola (Brassica napus L). Pak J Biol Sci 9:302–306Google Scholar
  20. Chandler SF, Thorpe TA (1987) Characterization of growth, water relations, and proline accumulation in sodium sulfate tolerant callus of Brassica napus L. cv. Westar (Canola). Plant Physiol 84:106–111CrossRefPubMedPubMedCentralGoogle Scholar
  21. Chaudhary S, Parmenter DL, Moloney MM (1998) Transgenic Brassica carinata as a vehicle for the production of recombinant proteins in seeds. Plant Cell Rep 17:195–200CrossRefGoogle Scholar
  22. Chikkala VR, Nugent GD, Dix PJ, Stevenson TW (2009) Regeneration from leaf explants and protoplasts of Brassica oleracea var. botrytis (cauliflower). Sci Hortic 119:330–334CrossRefGoogle Scholar
  23. Cho HS, Cao J, Ren JP, Earle ED (2001) Control of Lepidopteran insect pests in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) transformed with a synthetic Bacillus thuringiensis Cry1C gene. Plant Cell Rep 20:1–7CrossRefGoogle Scholar
  24. Daud NFA, Hasbullah NA, Azis NA, Rasad FM, Amin MAM, Lassim MM (2015) In vitro regeneration of Brassica oleracea var. capitata trough steams, roots, leaves and petioles cultures. In: International conference on agricultural, ecological and medical sciences (AEMS-2015), April, pp 7–83eGoogle Scholar
  25. Deng SY, Heap IM, Klein TA (1991) In vitro vegetative propagation of Chinese cabbage. Plant Cell Tiss Org Cult 26:135–139CrossRefGoogle Scholar
  26. Engineer CB, Fitzsimmons KC, Schmuke JJ, Dotson SB, Kranz RG (2005) Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer trap system in Arabidopsis. BMC Plant Biol 7:5–9Google Scholar
  27. Ertaș F, Tuncer B (2016) In vitro shoot and root regeneration in Ercis cabbage (Brassica oleraceae var. capitata). In: VII international scientific agriculture symposium, “Agrosym 2016”, Jahorina, Bosnia and Herzegovina, 6–9 Oct 2016. Proceedings. University of East Sarajevo, Faculty of Agriculture, pp 170–175Google Scholar
  28. Facciotti MT, Bertain PB, Yuan L (1999) Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase. Nat Biotechnol 17:593–597CrossRefGoogle Scholar
  29. Farzinebrahimi R, Taha RM, Fadainasaband M, Mokhtari S (2012) In vitro plant regeneration, antioxidant and antibacterial studies on broccoli, Brassica oleracea var. italica. Pak J Bot 44:2117–2122Google Scholar
  30. Gambhir G, Srivastava DK (2015) Thidiazuron induces high frequency shoot regeneration in leaf and petiole explants of cabbage (Brassica oleracea L. var. capitata). J Biotechnol Biomater 5:172Google Scholar
  31. Gambhir G, Kumar P, Srivastava DK (2017a) High frequency regeneration of plants from cotyledon and hypocotyl cultures in Brassica oleracea cv. pride of India. Biotechnol Rep 15:107–113CrossRefGoogle Scholar
  32. Gambhir G, Kumar P, Srivastava DK (2017b) Effect of antibiotic sensitivity on different cultured tissues and its significance in genetic transformation of cabbage Brassica oleracea. Biosci Biotechnol Res Commun 10:652–661CrossRefGoogle Scholar
  33. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158CrossRefGoogle Scholar
  34. Gantait S, Kundu S (2017a) In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiol Plant 39:196CrossRefGoogle Scholar
  35. Gantait S, Kundu S (2017b) Neoteric trends in tissue culture-mediated biotechnology of Indian ipecac [Tylophora indica (Burmf) Merrill]. 3 Biotech 7:231CrossRefPubMedPubMedCentralGoogle Scholar
  36. Gantait S, Mandal N (2010) Tissue culture of Anthurium andreanum: a significant review and future prospective. Int J Bot 6:207–219CrossRefGoogle Scholar
  37. Gantait S, El-Dawayati MM, Panigrahi J, Labrooy C, Verma SK (2018) The retrospect and prospect of the applications of biotechnology in Phoenix dactylifera L. Appl Microbiol Biotechnol 102:8229–8259CrossRefGoogle Scholar
  38. Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the ‘gene-jockeying’ tool. Microbiol Mol Biol Rev 67:16–37CrossRefPubMedPubMedCentralGoogle Scholar
  39. Gerszberg A, Hnatuszko-Konka K, Kowalczyk T (2015) In vitro regeneration of eight cultivars of Brassica oleracea var. capitata. In Vitro Cell Dev Biol Plant 51:80–87CrossRefGoogle Scholar
  40. Ghnaya AB, Charles G, Branchard M (2007) Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars of Brassica napus L. Plant Cell Tissue Organ Cult 92:25–30Google Scholar
  41. Godwin I, Todd G, Ford-Lloyd B, Newbury HJ (1991) The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species. Plant Cell Rep 9:671–675CrossRefGoogle Scholar
  42. Guo DP, Zhu ZJ, Hu XX, Zheng SJ (2005) Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai). Plant Cell Tiss Org Cult 83:123–127CrossRefGoogle Scholar
  43. Halfhill MD, Richards HA, Mabon SA, Stewart CN Jr (2001) Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapa. Theor Appl Genet 103:659–667CrossRefGoogle Scholar
  44. Hansen LN, Earle ED (1994) Novel flowering and fatty acid characters in rapid cycling Brassica napus L. synthesized by protoplast fusion. Plant Cell Rep 14:151–156PubMedGoogle Scholar
  45. Heath DW, Earle ED (1996) Synthesis of Ogura male sterile rapeseed (Brassica napus L.) with cold tolerance by protoplast fusion and effects of atrazine resistance on seed yield. Plant Cell Rep 15:939–944CrossRefGoogle Scholar
  46. Houmiel KL, Slater S, Broyles D, Casagrande L, Colburn S, Gonzalez K, Mitsky TA, Reiser SE, Shah D, Taylor NB (1999) Poly (beta-hydroxybutyrate) production in oilseed leukoplasts of Brassica napus. Planta 209:547–550CrossRefGoogle Scholar
  47. Hu Q, Anderson SB, Dixelius C, Hansen LN (2002) Production of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool. Plant Cell Rep 21:147–152CrossRefGoogle Scholar
  48. Hur SH, Min BW (2015) Efficient development of transgenic cabbage with jasmonic acid carboxyl methyltransferase (JMT) gene based on PMI/mannose selection system. Plant Breed Biotechnol 3:226–237CrossRefGoogle Scholar
  49. Hussein EA, Taj-Eldeen AM, Al-Zubairi AS, Elhakimi AS, Al-Dubaie AR (2010) Phytochemical screening, total phenolics and antioxidant and antibacterial activities of callus from Brassica nigra L. hypocotyl explants. Int J Pharmacol 6:464–471CrossRefGoogle Scholar
  50. Jagannath A, Bandyopadhyay P, Arumugam N, Gupta V, Burma PK, Pental D (2001) The use of a spacer DNA fragment insulates the tissue specific expression of a cytotoxic gene (barnase) and allows high frequency generation of transgenic male sterile lines in Brassica juncea L. Mol Breed 8:11–23CrossRefGoogle Scholar
  51. Jin RG, Liu YB, Tabashnik BE, Borthakur D (2000) Development of transgenic cabbage (Brassica oleracea var. capitata) for insect resistance by Agrobacterium tumefaciens-mediated transformation. In Vitro Cell Dev Biol Plant 36:231–237CrossRefGoogle Scholar
  52. Khan MR, Rashid H, Quraishi A (2002) Effects of various growth regulators on callus formation and regeneration in Brassica napus cv. Oscar. Pak J Biol Sci 5:693–695CrossRefGoogle Scholar
  53. Kirti PB, Narasimhulu SB, Prakash S, Chopra VL (1992) Production and characterization of intergeneric somatic hybrids of Trachystoma ballii and Brassica juncea. Plant Cell Rep 11:90–92CrossRefGoogle Scholar
  54. Klima M, Abraha E, Vyvadilova M, Bechyn M (2009) Protoplast culture and fusion between Brassica carinata and Brassica napus. Agricult Trop Subtrop 42:4–45Google Scholar
  55. Kohlenbach HW, Wenzel G, Hoffmann F (1982) Regeneration of Brassica napus plantlets in cultures from isolated protoplasts of haploid stem embryos as compared with leaf protoplasts. Z Pflanzenphysiol 105:131–142CrossRefGoogle Scholar
  56. Koo J, Kim Y, Kim J, Yeom M, Kumria R, Waie B, Rajam MV (2001) Plant regeneration from transformed embryogenic callus of elite Indica rice via Agrobacterium. Plant Cell Tiss Org Cult 67:63–71CrossRefGoogle Scholar
  57. Kranz E (1988) Somatic embryogenesis in stationary phase suspension cultures derived from hypocotyl protoplasts of Brassica napus L. Plant Cell Tiss Org Cult 12:141–146CrossRefGoogle Scholar
  58. Krikorian AD, Kelley K, Smith DI (1995) Hormones in tissue culture and micropropagation. In: Davies PJ (ed) Plant hormones. Physiology, biochemistry and molecular biology. Kluwer Academic, Dordrecht, pp 774–796CrossRefGoogle Scholar
  59. Kumar P, Srivastava DK (2016) Biotechnological applications in in vitro plant regeneration studies of broccoli (Brassica oleracea L. var. italica), an important vegetable crop. Biotechnol Lett 38:561–557CrossRefGoogle Scholar
  60. Kumar A, Sharma P, Thomas L, Agnihotri A, Banga SS (2009) Canola cultivation in India: scenario and future strategy. In: 16th Australian research assembly on Brassicas, Ballarat, pp 0–5Google Scholar
  61. Kuta DD, Tripathi L (2005) Agrobacterium-induced hypersensitive necrotic reaction in plant cells: a resistance response against Agrobacterium-mediated DNA transfer. Afr J Biotechnol 4:752–757Google Scholar
  62. Lee YH, Yoon IS, Suh SC, Kim HI (2002) Enhanced disease resistance in transgenic cabbage and tobacco expressing a glucose oxidase gene from Aspergillus niger. Plant Cell Rep 20:857–863CrossRefGoogle Scholar
  63. Lee YH, Chung KH, Kim HU, Jin YM, Kim HI, Park BS (2003) Induction of male sterile cabbage using a tapetum-specific promoter from Brassica campestris L. ssp. pekinensis. Plant Cell Rep 22:268–273CrossRefGoogle Scholar
  64. Lei JJ, Yang WJ, Yuan SH, Ying FY, Qiong LC (2006) Study on transformation of cysteine proteinase inhibitor gene into cabbage (Brassica oleracea var. capitata L.). Acta Hortic 706:231–238CrossRefGoogle Scholar
  65. Leroy XJ, Leon K, Charles G, Branchard M (2000) Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs. Plant Cell Rep 19:1102–1107CrossRefGoogle Scholar
  66. Li XB, Zheng SX, Dong WB, Chen GR, Mao HZ, Bai YY (1999) Insect-resistant transgenic plants of Brassica napus and analysis of resistance in the plants. Acta Genet Sin 26:262–268PubMedGoogle Scholar
  67. Li X, Peng RH, Fan HQ, Xiong AS, Yao QH, Cheng ZM, Li Y (2005) Vitreoscilla hemoglobin overexpression increases submergence tolerance in cabbage. Plant Cell Rep 23:710–715CrossRefGoogle Scholar
  68. Liu Clarke JH, Chevre AM, Landgren M, Glimelius K (1999) Characterization of sexual progenies of male sterile somatic cybrids between Brassica napus and Brassica tournefortii. Theor Appl Genet 99:605–610CrossRefGoogle Scholar
  69. Liu JW, DeMichele S, Bergana M, Bobik E, Hastilow C, Chuang LT, Mukerji P, Huang YS (2001) Characterization of oil exhibiting high gamma-linolenic acid from a genetically transformed canola strain. J Am Oil Chem Soc 78:489–493CrossRefGoogle Scholar
  70. Liu F, Ryschka U, Marthe F, Klocke E, Schumann G, Zhao H (2007a) Culture and fusion of pollen protoplasts of Brassica oleracea L. var. italica with haploid mesophyll protoplasts of B. rapa L. ssp. pekinensis. Protoplasma 231:89–97CrossRefGoogle Scholar
  71. Liu CW, Lin CC, Chen JJ, Tseng MJ (2007b) Stable chloroplast transformation in cabbage (Brassica oleracea L. var. capitata L.) by particle bombardment. Plant Cell Rep 26:1733–1744CrossRefGoogle Scholar
  72. Liu CW, Lin CC, Yiu JC, Chen JJ, Tseng MJ (2008) Expression of a Bacillus thuringiensis toxin (cry1Ab) gene in cabbage (Brassica oleracea L. var. capitata L.) chloroplasts confers high insecticidal efficacy against Plutella xylostella. Theor Appl Genet 117:75–88CrossRefGoogle Scholar
  73. Metz TD, Roush RT, Tang JD, Shelton AM, Earle ED (1995) Transgenic broccoli expressing a Bacillus thuringiensis insecticidal crystal protein – implications for pest resistance management strategies. Mol Breed 1:309–317CrossRefGoogle Scholar
  74. Miki B, McHugh S (2004) Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 107:193–232CrossRefGoogle Scholar
  75. Moghaieb REA, El-Awady MA, El Mergawy RG, Youssef SS, El-Sharkawy AM (2006) A reproducible protocol for regeneration and transformation in canola (Brassica napus L.). Afr J Biotechnol 5:143–148Google Scholar
  76. Munir M, Rashid H, Rauf M, Chaudhry Z, Bukhari MS (2008) Callus formation and plantlets regeneration from hypocotyl of Brassica napus by using different media combinations. Pak J Bot 40:309–315Google Scholar
  77. Munshi MK, Roy PK, Kabir MH, Ahmed G (2007) In vitro regeneration of cabbage (Brassica oleracea L. var. capitata) through hypocotyl and cotyledon culture. Plant Tissue Cult Biotechnol 17:131–136CrossRefGoogle Scholar
  78. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–495CrossRefGoogle Scholar
  79. Nagaharu U (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilisation. Jpn J Bot 7:389–452Google Scholar
  80. Odongo GA, Schlotz N, Herz C, Hanschen FS, Baldermann S, Neugart S, Lamy E (2017) The role of plant processing for the cancer preventive potential of Ethiopian kale (Brassica carinata). Food Nutr Res 61:1–11CrossRefGoogle Scholar
  81. Paul A, Sharma SR, Sresty TVS, Devi S, Bala S, Kumar PS, Saradhi PP, Frutos R, Altosaar I, Kumar PA (2005) Transgenic cabbage (Brassica oleracea var. capitata) resistant to Diamondback moth (Plutella xylostella). Indian J Biotechnol 4:72–77Google Scholar
  82. Pavlović S, Vinterhalter B, Mitić N, Adžić S, Pavlović N, Zdravković M, Vinterhalter D (2010) In vitro shoot regeneration from seedling explants in Brassica vegetables: red cabbage, broccoli, savoy cabbage and cauliflower. Arch Biol Sci 62:337–345CrossRefGoogle Scholar
  83. Pavlović S, Adžić S, Cvikić D, Zdravković J, Zdravković M, Pavlović S, Adžić S, Cvikić D, Zdravković J, Zdravković M (2012) In vitro culture as a part of Brassica oleracea var capitata L breeding. Genetika 44:611–618CrossRefGoogle Scholar
  84. Pental D, Pradhan AK, Sodhi YS, Mukhopadhyay A (1993) Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for Agrobacterium-mediated genetic transformation. Plant Cell Rep 12:462–467PubMedGoogle Scholar
  85. Pitzschke A, Hirt H (2010) New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation. EMBO J 29:1021–1032CrossRefPubMedPubMedCentralGoogle Scholar
  86. Prakash S, Kirti PB, Bhat SR (1998) A Moricandia arvensis-based cytoplasmic male sterility and fertility restoration system in Brassica juncea. Theor Appl Genet 97:488–492CrossRefGoogle Scholar
  87. Prasad KVSK, Sharmila P, Kumar PA, Saradhi PP (2000) Transformation of Brassica juncea (L.) Czern with bacterial coda gene enhances its tolerance to salt and cold stress. Mol Breed 6:489–499CrossRefGoogle Scholar
  88. Qamar Z, Jahangir GZ, Nasir IA, Husnain T (2014) In-vitro production of cabbage and cauliflower. Adv Life Sci 1:112–118Google Scholar
  89. Rafat A, Aziz MA, Rashid AA, Abdullah SNA, Kamaladini H, Sirchi MT, Javadi MB (2010) Optimization of Agrobacterium tumefaciens-mediated transformation and shoot regeneration after co-cultivation of cabbage (Brassica oleracea subsp. capitata) cv. KY Cross with AtHSP101 gene. Sci Hortic 124:1–8CrossRefGoogle Scholar
  90. Ravanfar SA, Aziz MA (2015) Shoot tip regeneration and optimization of Agrobacterium tumefaciens-mediated transformation of Broccoli (Brassica oleracea var. italica) cv. Green Marvel. Plant Biotechnol Rep 9:27–36CrossRefGoogle Scholar
  91. Ravanfar SA, Aziz MA, Kadir MA, Rashid AA, Sirchi MHT (2009) Plant regeneration of Brassica oleracea subsp. italica (Broccoli) cv. Green Marvel as affected by plant growth regulators. Afr J Biotechnol 8:2523–2528Google Scholar
  92. Ravanfar SA, Aziz MA, Kadir MA, Rashid AA, Haddadi F (2011) In vitro adventitious shoot regeneration and acclimatisation of Brassica oleracea subsp. italica cv. Green Marvel. Afr J Biotechnol 10:5614–5619Google Scholar
  93. Ravanfar SA, Salim S, Aziz MA, Abdullah SNA, Rashid AA (2014) Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) “KY Cross”. Plant Biotechnol 31:275–280CrossRefGoogle Scholar
  94. Ravanfar SA, Aziz MA, Saud HM, Abdullah JO (2015) Optimization of in vitro regeneration and Agrobacterium tumefaciens-mediated transformation with heat-resistant cDNA in Brassica oleracea subsp. italica cv. Green Marvel. Curr Genet 61:653–663CrossRefGoogle Scholar
  95. Ravanfar SA, Orbovic V, Moradpour M, Abdul Aziz M, Karan R, Wallace S, Parajuli S (2017) Improvement of tissue culture, genetic transformation, and applications of biotechnology to Brassica. Biotechnol Genet Eng Rev 33:1–25CrossRefGoogle Scholar
  96. Redden R, Vardy M, Edwards D, Raman H, Batley J (2009) Genetic and morphological diversity in the Brassicas and wild relative. In: 16th Australian research assembly on Brassicas, Ballarat, Victoria. Retrieved from
  97. Ren JP, Dickson MH, Earle ED (2000) Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea. Theor Appl Genet 100:810–819CrossRefGoogle Scholar
  98. Rodrigues AADJ, Santos EDO, Takane RJ, Carvalho ACPPD (2017) Artificial light and growth regulators on the in vitro etiolation of Cattleya labiata. Rev Ciênc Agron 48:296–302Google Scholar
  99. Rout GR, Mohapatra A, Jain S (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24:531–560CrossRefGoogle Scholar
  100. Sharma S, Gambhir G, Srivastava DK (2014) High frequency organogenesis in cotyledon and hypocotyl explants of cabbage (Brassica oleracea L. var. capitata). Natl Acad Sci Lett 37:5–12CrossRefGoogle Scholar
  101. Shekhawat GS, Verma K, Jana S, Singh K, Teotia P, Prasad A (2010) In vitro biochemical evaluation of cadmium tolerance mechanism in callus and seedlings of Brassica juncea. Protoplasma 239:31–38CrossRefGoogle Scholar
  102. Shewmaker CK, Sheehy JA, Daley M, Colburn S, Ke DY (1999) Seed specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects. Plant J 20:401–412CrossRefGoogle Scholar
  103. Sigareva MA, Earle ED (1997) Direct transfer of a cold-tolerant Ogura male sterile cytoplasm into cabbage (Brassica oleracea ssp. capitata) via protoplast fusion. Theor Appl Genet 94:213–220CrossRefGoogle Scholar
  104. Stanišić M, Ninković S, Savić J, Ćosić T, Mitić N (2018) The effects of β-lactam antibiotics and hygromycin B on de novo shoot organogenesis in apple cv. golden delicious. Arch Biol Sci 70:179–190CrossRefGoogle Scholar
  105. Steinmetz KA, Potter JD (1996) Vegetables, fruit, and cancer prevention: a review. J Am Diet Assoc 96:1027–1039CrossRefGoogle Scholar
  106. Stewart CN, Adang MJ, All JN, Raymer PL, Ramachandran S, Parrott WA (1996) Insect control and dosage effects in transgenic canola containing a synthetic Bacillus thuringiensis cry1Ac gene. Plant Physiol 112:115–120CrossRefGoogle Scholar
  107. Stretnović-Rajičić T, Ninkowić S, Miljuš-Dukić J, Vinterhalter B, Vinterhalter D (2006) Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B. oleracea var. capitata. Biol Plant 50:525–530CrossRefGoogle Scholar
  108. Tang GX, Zhou WJ, Li HZ, Mao BZ, He ZH, Yoneyama K (2003) Medium, explant and genotype factors influencing shoot regeneration in oilseed Brassica spp. J Agron Crop Sci 189:351–358CrossRefGoogle Scholar
  109. Tsukazaki H, Kuginuki Y, Aida R, Suzuki T (2002) Agrobacterium-mediated transformation of a doubled haploid line of cabbage. Plant Cell Rep 21:257–262CrossRefGoogle Scholar
  110. Turgut K, Barghchi M, Scott R (1998) Efficient shoot regeneration and somatic embryogenesis from immature cotyledons of Brassica napus L. Plant Breed 117:503–504CrossRefGoogle Scholar
  111. Van Dorst B, Mehta J, Rouah-Martin E, Backeljau J, De Coen W, Eeckhout D, Robbens J (2011) Selection of scFv phages specific for chloramphenicol acetyl transferase (CAT), as alternatives for antibodies in CAT detection assays. J Appl Toxicol 32:783–789CrossRefGoogle Scholar
  112. Wang YP, Sonntag K, Rudloff E (2003) Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica. Theor Appl Genet 106:1147–1155CrossRefGoogle Scholar
  113. Wang YP, Sonntag K, Rudloff E, Chen JM (2005) Intergeneric somatic hybridization between Brassica napus L. and Sinapis alba L. J Integr Plant Biol 47:84–91CrossRefGoogle Scholar
  114. Xiang Y, Wong WKR, Ma MC, Wong RSC (2000) Agrobacterium mediated transformation of Brassica campestris ssp. parachinensis with synthetic Bacillus thuringiensis Cry1Ab and Cry1Ac genes. Plant Cell Rep 19:251–256CrossRefGoogle Scholar
  115. Yamagishi H, Landgren M, Forsberg J, Glimelius K (2002) Production of asymmetric hybrids between Arabidopsis thaliana and Brassica napus utilizing an efficient protoplast culture system. Theor Appl Genet 104:959–964CrossRefGoogle Scholar
  116. Yan Z, Tian Z, Huang B, Huang R, Meng J (1999) Production of somatic hybrids between Brassica oleracea and the C3-C4 intermediate species Moricandia nitens. Theor Appl Genet 99:1281–1286CrossRefGoogle Scholar
  117. Yang MZ, Jia SR, Pua EC (1991) High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br. Plant Cell Tiss Org Cult 24:79–82CrossRefGoogle Scholar
  118. Yi D, Lei C, Li Y, Zhuang M, Zhang Y, Fang Z, Yang L (2011) Transformation of cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 gene for control of Diamondback Moth. Agric Sci China 10:1693–1700CrossRefGoogle Scholar
  119. Yi D, Cui L, Wang L, Liu Y, Zhuang M, Zhang Y, Zhang J, Lang Z, Zhang Z, Fang Z, Yang L (2013) Pyramiding of Bt cry1Ia8 and cry1Ba3 genes into cabbage (Brassica oleracea L. var. capitata) confers effective control against diamond back moth. Plant Cell Tiss Org Cult 115:419–428CrossRefGoogle Scholar
  120. Zhang HX, Hodson JN, Williams JP, Blumwald E (2001) Engineering salt tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci USA 98:12832–12836CrossRefGoogle Scholar
  121. Zhao S, Lei JJ, Chen GJ, Cao BH (2008) Application of kanamycin in transgenic mustard (Brassica juncea Coss.). Heretidas 30:501–507Google Scholar
  122. Zhong R, Zhu F, Liu YL, Li SG, Kang LY, Luo P (1997) Oilseed rape transformation and the establishment of a bromoxynil-resistant transgenic oilseed rape. Acta Bot Sin 39:22–27Google Scholar

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaMohanpur, NadiaIndia
  2. 2.Crop Research Unit (Genetics and Plant Breeding)Bidhan Chandra Krishi ViswavidyalayaMohanpur, NadiaIndia

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