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Tissue Culture-Mediated Biotechnological Advancements in Genus Brassica

  • Monisha Mitra
  • Saikat GantaitEmail author
Chapter
  • 41 Downloads

Abstract

Brassica is documented as a distinguished genus, comprising a wide array of species, belonging to the family Brassicaceae that are majorly consumed as vegetables and processed for oil and condiments. Conventional breeding approaches are laborious; in addition, higher environmental influence interferes with the yield and quality of crop; hence, biotechnological intervention becomes a major requirement. Till date, a lot of studies have been carried out on biotechnological advancements of genus Brassica. Among those, tissue culture-mediated approaches, such as development of protocols for in vitro mass propagation, somaclonal variation, and secondary metabolites production, are highlighted in this chapter. Additionally, genetic transformation and protoplast culture, the other two major tissue culture-based methods, which were substantially used for genetic improvement of Brassica, have also been discussed.

Keywords

Agrobacterium Callus Genetic transformation Micropropagation Protoplast culture Somatic embryogenesis 

Abbreviations

2,4-D

2,4-dichlorophenoxy acetic acid

B5

Gamborg’s medium

BA

N6-benzyladenine

BAP

N6-benzylaminopurine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

Kinetin

6-furfurylaminopurine

MS

Murashige and Skoog medium

NAA

α-Naphthalene acetic acid

PGR

Plant growth regulator

SE

Somatic embryo

TDZ

N-phenyl-N′-(1,2,3-thidiazol-5-yl) urea or Thidiazuron

Zeatin

4-hydroxy-3-methyl-terms-2-butenyl aminopurine

Notes

Acknowledgements

The authors acknowledge the e-library assistance from the Central Library, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, West Bengal, India. Further, they are thankful to the anonymous reviewers and the editor of this chapter for their critical comments and suggestions on the manuscript.

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureBidhan Chandra Krishi ViswavidyalayaMohanpur, NadiaIndia
  2. 2.Crop Research Unit (Genetics and Plant Breeding)Bidhan Chandra Krishi ViswavidyalayaMohanpur, NadiaIndia

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