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Structure and Function of Molecular Chaperones that Govern Immune Peptide Loading

  • David H. MarguliesEmail author
  • Jiansheng Jiang
  • Kannan Natarajan
Chapter
Part of the Subcellular Biochemistry book series (SCBI, volume 93)

Abstract

Major histocompatibility class I (MHC-I) molecules bind peptides derived from cellular synthesis and display them at the cell surface for recognition by receptors on T lymphocytes (TCR) or natural killer (NK) cells. Such recognition provides a crucial step in autoimmunity, identification of bacterial and viral pathogens, and anti-tumor responses. Understanding the mechanism by which such antigenic peptides in the ER are loaded and exchanged for higher affinity peptides onto MHC molecules has recently been clarified by cryo-EM and X-ray studies of the multimolecular peptide loading complex (PLC) and a unimolecular tapasin-like chaperone designated TAPBPR. Insights from these structural studies and complementary solution NMR experiments provide a basis for understanding mechanisms related to immune antigen presentation.

Keywords

Immune recognition Major histocompatibility complex Chaperone Tapasin TAPBPR Protein loading complex Structural biology X-ray crystallography 

Abbreviations

MHC

Major histocompatibility complex

MHC-I

MHC class I

MHC-II

MHC class II

TCR

T cell receptor

NK

Natural killer

ER

Endoplasmic reticulum

PLC

Peptide loading complex

TAP

Transporter associated with antigen processing

TAPBP or tapasin

TAP binding protein

TAPBPR

TAP binding protein, related

NMR

Nuclear magnetic resonance

Notes

Acknowledgements

This research was supported by the Intramural Research Program of the National Institutes of Health, NIAID.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • David H. Margulies
    • 1
    Email author
  • Jiansheng Jiang
    • 1
  • Kannan Natarajan
    • 1
  1. 1.Molecular Biology Section, Laboratory of Immune System BiologyNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

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