Plant Microbe Interface: The Plant Antimicrobial Peptides

  • S. ManivannanEmail author
  • P. Umadevi


Antimicrobial peptides (AMPs) are known to play important roles in plant development and stress tolerance. The AMPs are involved in the defense reaction of innate plant immunity and are known to increase the transcription level in response to abiotic or biotic stress factors. There are numerous reports available on the structure and the in vitro efficiency of antimicrobial peptides against phytopathogens. This chapter provides the available pipelines to identify the plant AMPs and its role in mediating the defense in plants in particular.


Peptides Regulation Defense Signature tags 


  1. Asiegbu FO, Choi W, Li G, Nahalkova J, Dean RA (2003) Isolation of a novel antimicrobial peptide gene (SpAMP) homologue from Pinus sylvestris (scots pine) following infection with the root rot fungus Heterobasidion annosum. FEMS Microbiol Lett 228:27–31CrossRefGoogle Scholar
  2. Cammue BPA, De Bolle MFC, Terras FRG, Proost P, Van Damme J, Rees SB, Vanderleyden J, Broekaert WF (1992) Isolation and characterization of a novel class of plant antimicrobial peptides from Mirabilis jalapa L. seeds. J Biol Chem 267:2228–2233PubMedGoogle Scholar
  3. Egorov TA, Odintsova TI, Vitaliy A, Pukhalsky VA, Grishin EV (2005) Diversity of wheat anti-microbial peptides. Peptides 26:2064–2073CrossRefGoogle Scholar
  4. Gillon AD, Saska I, Jennings CV, Guarino RF, Craik DJ, Anderson MA (2008) Biosynthesis of circular proteins in plants. Plant J 53:505–515CrossRefGoogle Scholar
  5. Huffaker A, Ryan CA (2007) Endogenous peptide defense signals in Arabidopsis differentially amplify signaling for the innate immune response. Proc Natl Acad Sci USA 104:10732–10736CrossRefGoogle Scholar
  6. Huffaker A, Pearce G, Ryan CA (2006) An endogenous peptide signal in Arabidopsis activates components of the innate immune response. Proc Natl Acad Sci USA 103:10098–10103CrossRefGoogle Scholar
  7. Huffaker A, Dafoe NJ, Schmelz EA (2011) ZmPep1, an Ortholog of Arabidopsis elicitor peptide 1, regulates maize innate immunity and enhances disease resistance. Plant Physiol 155:1325–1338CrossRefGoogle Scholar
  8. Ke T, Cao H, Huang J, Hu F, Huang J, Dong C, Ma X, Yu J, Mao H, Wang X, Niu Q, Hui F, Liu S (2015) EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus. BMC Genomics 16:653CrossRefGoogle Scholar
  9. Kovtun A, Shelenkov A, Odintsova T (2018) The diversity of putative antimicrobial peptides revealed in wheat by high throughput next generation transcriptome sequencing. 43rd FEBS Congress, PragueGoogle Scholar
  10. Mirouze M, Sels J, Richard O, Czernic P, Loubet S, Jacquier A, François IEJA, Cammue BPA, Lebrun M, Berthomieu P, Marque L (2006) A putative novel role for plant defensins: a defensin from the zinc hyper-accumulating plant, Arabidopsis halleri, confers zinc tolerance. Plant J 47:329–342CrossRefGoogle Scholar
  11. Nawrot R, Barylski J, Nowicki G, Broniarczyk J, Buchwald W, Gozdzicka-Jozefiak A (2014) Plant antimicrobial peptides. Folia Microbiol 59(3):181–196CrossRefGoogle Scholar
  12. Park CJ, Park CB, Hong SS, Lee HS, Lee SY, Kim SC (2000) Characterization and cDNA cloning of two glycine- and histidine-rich antimicrobial peptides from the roots of shepherd’s purse, Capsella bursa-pastoris. Plant Mol Biol 44:187–197CrossRefGoogle Scholar
  13. Pearce G, Yamaguchi Y, Barona G, Clarence A (2010) Ryan A subtilisin-like protein from soybean contains an embedded, cryptic signal that activates defense related genes. PNAS 107(33):14921–14925CrossRefGoogle Scholar
  14. Ramada MHS, Brand GD, Abrão FY, Oliveira M, Cardozo Filho JL, Galbieri R, Gramacho KP, Prates MV, Bloch C Jr (2017) Encrypted antimicrobial peptides from plant proteins. Sci Rep 7:13263CrossRefGoogle Scholar
  15. Salas CE, Badillo-Corona JA, Ramírez-Sotelo G, Oliver-Salvador C (2014) Biologically active and antimicrobial peptides from plants. Biomed Res Int 2015:11Google Scholar
  16. Silva ON, Porto WF, Migliolo L, Mandal SM, Gomes DG, Holanda HH, Silva RS, Dias SC, Costa MP, Costa CR, Silva MR, Rezende TM, Franco OL (2012) Cn-AMP1: a new promiscuous peptide with potential for microbial infections treatment. Biopolymers 98(4):322–331CrossRefGoogle Scholar
  17. Slavokhotova AA, Shelenkov AA, Odintsova TI (2015) Prediction of Leymus arenarius (L.) antimicrobial peptides based on de novo transcriptome assembly. Plant Mol Biol 89:203–214CrossRefGoogle Scholar
  18. Umadevi P, Soumya M, George JK, Anandaraj M (2018) Proteomics assisted profiling of antimicrobial peptide signatures from black pepper (Piper nigrum L.). Physiol Mol Biol Plants 24(3):379–387CrossRefGoogle Scholar
  19. Utkina LL, Yaroslav AA, Eugene AR, Tatyana VK, Anna AS, Peter BO, Vassilevski AA, Eugene VG, Tsezi AE, Tatyana IO (2013) Genes encoding 4-Cys antimicrobial peptides in wheat Triticum kiharae Dorof. et Migush.: multimodular structural organization, intraspecific variability, distribution and role in defence. FEBS J 280:3594–3608CrossRefGoogle Scholar
  20. Wang Q, Yanga S, Liua J, Terecskeib K, Ábrahámb E, Gombárc A, Domonkosc A, Szucsb A, Körmöczib P, Wangb T, Fodorc L, Maod L, Feid Z, Kondorosib E, Kalóc P, Keresztb A, Zhua H (2017) Host-secreted antimicrobial peptide enforces symbiotic selectivity in Medicago truncatula. PNAS 114(26):6854–6859PubMedGoogle Scholar
  21. Yamaguchi Y, Pearce G, Ryan CA (2006) The cell surface leucine-rich repeat receptor for AtPep1, an endogenous peptide elicitor in Arabidopsis, is functional in transgenic tobacco cells. Proc Natl Acad Sci U S A 103:10104–10109CrossRefGoogle Scholar
  22. Yamaguchi Y, Huffaker A, Bryan AC, Tax FE, Ryan CA (2010) PEPR2 is a second receptor for the Pep1 and Pep2 peptides and contributes to defense responses in Arabidopsis. Plant Cell 22:508–522CrossRefGoogle Scholar
  23. Zipfel C (2009) Early molecular events in PAMP-triggered immunity. Curr Opin Plant Biol 12:414–420CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of HorticultureSikkim UniversityGangtokIndia
  2. 2.Division of Crop Improvement and Biotechnology, ICAR-Indian Institute of Spices ResearchKozhikodeIndia

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