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Circulating miRNAs as Biomarker in Cancer

  • Gitte Brinch Andersen
  • Jörg TostEmail author
Chapter
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 215)

Abstract

Deregulation of microRNA expression has been shown to play an important role in human malignancies. The identification of circulating-free miRNAs in biofluids a decade ago led to great enthusiasm and motivation to develop non-invasive tests based on the expression of these small non-coding RNAs. Herein, we review the progress within the field of research for identifying circulating miRNA cancer biomarkers and discuss the advantages and challenges associated with this. We also discuss the methodological and analytical variables, which may influence the final miRNA quantification and the importance of standardizing pre-analytical, analytical, and post-analytical processes in order to enable a successful translation of the results from basic research into the clinics.

Keywords

miRNA Cancer Biomarker Biofluids Precision medicine Diagnosis Prognosis 

Abbreviations

EDTA

Ethylenediaminetetraacetic acid

HDL

High-density lipoproteins

LDCT

Low-dose computed tomography

MGCT

Malignant germ cell tumors

miRNA

MicroRNA

MSC

miRNA signature classifier

Nt

Nucleotides

qPCR

Quantitative polymerase chain reaction

snoRNA

Small nucleolar RNA

UTR

Untranslated region

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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Department of BiomedicineAarhus UniversityAarhus CDenmark
  2. 2.Laboratory for Epigenetics and EnvironmentCentre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Bâtiment G2EvryFrance

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