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Biocatalysis pp 113-138 | Cite as

Immobilization of α-amylases and Their Analytical Applications

  • Om Prakash
  • Saumya Khare
Chapter

Abstract

The enzyme stability is a key parameter for its analytical applications. In this backdrop, the current chapter gives an insight about enzyme immobilization using various strategies highlighting its pros and cons. The chapter provides an overview about industrial application of immobilized α-amylase on various matrices with major focus on emerging technologies. The nanotechnology coupled with biotechnological advances provides new avenue in the area of enzyme immobilization. The chapter reveals the excellent properties of nanomaterials as efficient matrices for enzyme immobilization with enhanced catalytic activity, stability and reusability leading the process economically viable and environmentally feasible. The immobilized α-amylase on nanomatrices possess excellent properties contrary to their soluble forms are more preferred for industrial processes. Hence, the present chapter gives an idea about the ongoing research on industrially important α-amylase highlighting new approaches of nanotechnology for enzyme immobilization, which suggests its efficient role for various analytical applications.

Keywords

Alpha-amylase Enzyme immobilization Nanomaterial matrices Industrial application 

Abbreviations

AFSMNPs

Amino-functionalized silica-coated magnetite nanoparticles

AAM

α-amylase

AgNP

Ag nanoparticles

AgNP/ESM

Ag-nanoparticle-decorated eggshell membrane

Ca+2(Alg.St/PEI/GA)

Calcium (alginate.starch/polyethyleneimine/glutaraldehyde)

CLEA

Crosslinked enzyme aggregate

DEAE

Diethylaminoethylcellulose

EE

Encapsulated enzyme

ESM

Eggshell membrane

GNRs

Gold nanorods

HEMA-GMA

1-3 (hydroxyethylmethacrylate glycidyl methacrylate)

IPDI

Isophorone diisocyanate

LDH

Layered double-hydroxide

LOF

Luffa operculata fibre

M-CLEAs

Macromolecular cross-linked enzyme aggregates

MPIA

Magnetic particles immobilization α-amylase

MMIP

Magnetic Molecular Imprinted Polymer

NA

Naringin

NMOs

Nanostructured metal oxides

PPA

Porcine pancreatic α-amylase

PANI

Polyaniline

PEMA

3-D poly(ethylene-alt-maleic anhydride)

POMA

Poly(octadecene-alt-maleic anhydride)

PPyAgNp

Polypyrrole/silver nanocomposite

PUU

Polyurethane urea

PVA

Poly(vinyl alcohol)

PVA/PAA

Poly vinyl alcohol/poly acrylic acid

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Om Prakash
    • 1
  • Saumya Khare
    • 1
  1. 1.Department of Biochemistry, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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