β-Amylase: General Properties, Mechanism and Panorama of Applications by Immobilization on Nano-Structures

  • Ranjana Das
  • Arvind M. Kayastha


The present chapter describes the enzyme β-amylase in detail so as to get a better understanding of its structure, function, reaction mechanism and potential utility in industry. Main function of the enzyme in plant is starch degradation. The enzyme hydrolyzes α,1-4 glycosidic linkages in starch and related polysaccharide and is distributed among plants, microorganism and fungi. β-Amylases require a minimum chain length of four glycosyl residues. Therefore, the final products of the degradation of linear glucan chains are maltose (4-O-α-D-glucopyranosyl-β-D-glucose) and a small amount of maltotriose, which is too short to be hydrolyzed by β-amylases. Degradation of branched polymers yield maltose and isomaltose as the enzyme cannot bypass α,1-6 linkages. Structure determination by X-ray crystallography of sweet potato and soybean β-amylases efficiently described the amino acid residues involved in catalysis. Detailed classifications of plant β-amylases have been discussed in this chapter which reveals that β-amylases are the major enzyme of starch degradation pathway. The enzyme finds application in industry as the end product maltose is indispensable as sweetener in food and pharmaceuticals because of its mild sweetness and lack of color formation. β-Amylases also delay starch retrogradation, thus maintaining the quality of bread and preventing it from staling. In addition, applications and future prospects of industrially important β-amylase enzyme, immobilized on various matrices with potential for biotechnological applications are discussed in detail.


β-Amylases Immobilized enzymes Catalytic applications Hydrolysis 


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Ranjana Das
    • 1
  • Arvind M. Kayastha
    • 1
  1. 1.School of Biotechnology, Institute of ScienceBanaras Hindu UniversityVaranasiIndia

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