Flax Small RNAs
Small RNAs (short RNA molecules, generally, 20-24 nucleotides in length) are the key regulators of numerous biological processes in plants. The most extensively studied small RNAs in plant species are microRNAs (miRNAs), which regulate gene expression at the post-transcriptional level. In flax, miRNAs were predicted on the basis of genome and transcriptome sequencing data. Moreover, high-throughput sequencing of small RNAs and evaluation of miRNA levels using quantitative PCR allowed determination of miRNAs in various tissues and under stress conditions and identification of differentially expressed miRNAs. Furthermore, the target genes of miRNAs were predicted. Obtained results brought important knowledge about the regulation of cell processes in flax plants via miRNA. However, further analysis covering more classes of small RNAs is needed.
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