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Microbiology of the Laetolil Tuff 7 with 3.66 Ma Australopithecus Afarensis Footprints, Ngorongoro Conservation Area, Tanzania

  • Anatoly N. ZaitsevEmail author
  • Dmitry Yu. Vlasov
  • Marina S. Zelenskaya
  • Olga A. Zaitseva
  • Olga A. Pavlova
  • Anton R. Chakhmouradian
  • Anton I. Savchenok
  • Larissa Leach
  • Michael Leach
  • Joshua Mwankunda
Conference paper
Part of the Lecture Notes in Earth System Sciences book series (LNESS)

Abstract

East Africa is one of the most important paleoanthropological localities on Earth. Laetoli and Olduvai Gorge (Tanzania) are among the world’s premier areas for Australopithecus afarensis, Paranthropus boisei, and Homo habilis remains. Laetoli is also unique in its preservation of footprint trails within Tuff 7 left by Australopithecus afarensis. Tuff 7 has been studied to characterize microorganisms living inside these tuffs and to estimate their potential involvement in destructive processes; 35 species of microfungi, as well as sterile white and dark mycelia were identified by cultural method. The results of the metagenomic analysis show that anamorphic ascomycetes are the predominant group in all samples. They occur as hyphae and mycelia inside the tuffs, and are concentrated in pores, microcracks and cavities. The number of micromycetes is moderate (up to 7000 CFU per gram of substrate). Typical cultivated microfungi are from genera Aspergillus and Fusarium. They are known as active destructors of natural and artificial substrates and can colonize building materials. Molecular genetic methods revealed a large group of different bacteria (23 phyla) within the tuff. The microbiota consists mostly of Actinobacteria, Proteobacteria, Bacteroidetes and Cyanobacteria. Their relative distribution shows the preferential occurrence of Bacteroidetes in the upper part of stratigraphic sections (soil), and concentration of Actinobacteria and Proteobacteria within the tuffs. Exposure of the Footprint tuff could lead to the development of photosynthetic microorganisms (Cyanobacteria). We conclude that microbiological activity within the study area appears to be moderate and the Footprint Tuff does not presently require any treatment with biocides. However, the presence of black biofilms on the surface of the Footprint conservation mound concrete shows that biodestruction does occur.

Keywords

Australopithecus afarensis Footprint Tuff Microfungi Bacteria Laetoli Tanzania 

Notes

Acknowledgements

We thank M.N. Pagolskaya (St. Petersburg) and J.S. (J4) Pyuza (GMP Consulting Engineers Ltd.) for help during field work at Laetoli. Figure 36.1 is adapted by permission from the Jet Propulsion Laboratory (California Institute of Technology). We would like to thank the Tanzania Commission for Science and Technology for granting permission to conduct research (2016-245-NA-2016-181, 2017-234-NA-2016-115 for A.N. Zaitsev, 2016-242-NA-2016-181 for A.I. Savchenok). Field work at Laetoli was supported by Peter Rich Architects—GMP Consulting Engineers—Laetoli JV (Arusha), St. Petersburg State University (grants 0.42.955.2016 and 3.42.740.2017) and Ngorongoro Conservation Area Authorities (Tanzania). At various stages, this research was funded by the Peter Rich Architects—GMP Consulting Engineers—Laetoli JV (Arusha), Natural History Museum (London) and St. Petersburg State University (Resource Centres for X-ray Diffraction Studies, Geo-Environmental Research and Modelling, and Molecular and Cell Technologies and Culture Collection of Microorganisms, grant 3.20.1855.2015). The research results were partially obtained on the equipment of the Resource Center “Development of Cellular and Molecular Technologies” of St. Petersburg State University.

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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Anatoly N. Zaitsev
    • 1
    • 3
    Email author
  • Dmitry Yu. Vlasov
    • 2
  • Marina S. Zelenskaya
    • 2
  • Olga A. Zaitseva
    • 4
  • Olga A. Pavlova
    • 5
    • 9
  • Anton R. Chakhmouradian
    • 6
  • Anton I. Savchenok
    • 1
  • Larissa Leach
    • 7
  • Michael Leach
    • 7
  • Joshua Mwankunda
    • 8
  1. 1.Department of MineralogySaint Petersburg State UniversitySaint PetersburgRussia
  2. 2.Saint Petersburg State UniversitySaint PetersburgRussia
  3. 3.Image and Analysis Centre, The Natural History MuseumLondonUK
  4. 4.The Manege, Central Exhibition HallSaint PetersburgRussia
  5. 5.Beagle Ltd.Saint PetersburgRussia
  6. 6.Department of Geological SciencesUniversity of ManitobaWinnipegCanada
  7. 7.GMP Consulting Engineers Ltd.ArushaTanzania
  8. 8.Ngorongoro Conservation Area AuthorityArushaTanzania
  9. 9.Resource Center “Development of Cellular and Molecular Technologies”, Saint Petersburg State UniversitySaint PetersburgRussia

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