Bio-inert Interactions in an Oil—Microorganisms System

  • Lidia I. SvarovskayaEmail author
  • Andrey Y. Manakov
  • Lyubov K. Altunina
  • Larisa A. Strelets
Conference paper
Part of the Lecture Notes in Earth System Sciences book series (LNESS)


Most microorganisms are capable of the enzymatic oxidation of petroleum hydrocarbons and their use as a source of nutrition and energy. In the laboratory, enzymatic oil oxidation (biodegradation) in a liquid mineral medium is carried out with constant stirring for 30 and 60 days. The biodegradation of oil is carried out by a community of hydrocarbon-oxidizing microorganisms isolated from the composition of crude oil. The enzymatic activity of isolates is investigated individually. The paper presents the data of biodestructive changes in the physicochemical properties of oil, its group and component composition at different stages of biodegradation. Particular attention is paid to changes in the content of polar components in the composition of biodegraded oils. It is found that a ‘water in oil’ emulsion is formed in the course of cultivation of microorganisms in contact with oil. In this case, changes in the interfacial tension and pour point of oil and increase in its viscosity and density are observed. An analysis of samples of crude and biodegraded oil by the method of IR spectrometry showed that the degree of hydrocarbon oxidation and the content of aromatic structures (C=C), resins, and asphaltenes increase with increasing time of contact with microorganisms. The accumulation of oxygen-containing compounds (C=O) confirms the destructive activity of microorganisms. Emulsions of crude and biodegraded oil samples were under study in the course of nucleation of hydrates, depending on the degree of oxidation against the background of changes in the group composition of oil, the content of acids, resins and asphaltenes, and an increase in viscosity and density.


Oil Microorganisms Biodegradation Gas hydrates 


  1. Aman ZM, Koh CA (2016) Interfacial phenomena in gas hydrate systems. Chem Soc Rev 45:1678–1690. Scholar
  2. Augustinovic Z, Birketveit O, Clements K et al (2012) Microbes—oilfield enemies or allies. Oilfield Rev 24(2):4–17Google Scholar
  3. Barth T, Hoiland S, Fotland P et al (2004) Acidic compounds in biodegraded petroleum. Org Geochem 35(11–12):1513–1525. Scholar
  4. Borgund AE, Hoiland S, Barth T et al (2009) Molecular analysis of petroleum derived compounds that adsorb onto gas hydrate surfaces. Appl Geochem 24(5):777–786CrossRefGoogle Scholar
  5. Chen L, Feng Y, Okajima J et al (2018) Production behavior and numerical analysis for 2017 methane hydrate extraction test of Shenhu, South China Sea. J Nat Gas Sci Eng 53:55–66CrossRefGoogle Scholar
  6. Erstad K, Hvidsten IV, Askvik KM et al (2009) Changes in crude oil composition during laboratory biodegradation: acids and oil-water. Oil-Hydr Interfacial Prop Energy Fuels 23(8):4068–4076. Scholar
  7. Gerhcardt Ph (ed) (1981) Manual of methods for general bacteriology. American Society for Microbiology, WashingtonGoogle Scholar
  8. Greaves D, Boxall J, Mulligan J et al (2008) Hydrate formation from high water content—crude oil emulsions. Chem Eng Sci 63(18):4570–4579. Scholar
  9. Harayama S, Kishira H, Kasai Yu et al (1999) Petroleum biodegradation in marine environments. J Molec Microbiol Biotechnol 1(1):63–70Google Scholar
  10. Hoiland S, Borgund AE, Barth T et al (2005a) Wettability of Freon hydrates in crude oil/brine emulsions: the effects of chemical additives. In: Proceeding of 5th international conference on Gas Hydrate, Trondheim, Tapir Academic Press, Norway, 13–16 June 2005CrossRefGoogle Scholar
  11. Hoiland S, Askvik KM, Fotland P et al (2005b) Wettability of Freon hydrates in crude oil/brine emulsions. J Coll Interf Sci 287(1):217–225. Scholar
  12. Kapellos GE (2017) Microbial strategies for oil biodegradation. In: Becker SM (ed) Modeling of microscale transport in biological processes, 1st ed. Academic Press, Cambridge, Massachusetts, pp 19–39CrossRefGoogle Scholar
  13. Kelland MA (2006) History of the development of low dosage hydrate inhibitors. Energy Fuel 20(3):825–847CrossRefGoogle Scholar
  14. Koshelev VN, Gordadze GN, Ryabov VD et al (2005) Transformations of crude oils in in-situ combustion and prolonged contact with the environment. Chem Technol Fuels Oils 41(2):104–107. Scholar
  15. Rogers R, Radich J, Hiong S (2011) The multiple roles of microbes in the formation, dissociation and stability of seafloor gas hydrates. In: Proceedings of the 7th international conference on gas hydrates (ICGH). Edinburg, Scotland, United Kingdom, 17–21 July 2011Google Scholar
  16. Sloan ED, Koh CA (2007) Clathrate hydrates of natural gases, 3rd edn. CRC Press, Boca RatorCrossRefGoogle Scholar
  17. Speight GJ, Arjoon KK (2012) Bioremediation of petroleum and petroleum products. First published, Wiley-ScrivenerGoogle Scholar
  18. Stoporev AS, Manakov AY, Altunina LK et al (2015) Nucleation rates of methane hydrate from water in oil emulsions. Can J Chem 93(8):882–887. Scholar
  19. Stoporev AS, Svarovskaya LI, Strelets LA et al (2018a) Effect of reactor wall material on the nucleation of methane hydrate in water-in-oil emulsions. Mendeleev Commun 28(3):343–344. Scholar
  20. Stoporev AS, Ogienko AG, Sizikov AA et al (2018b) Unexpected formation of sII methane hydrate in some water-in-oil emulsions: different reasons for the same phenomenon. J Nat Gas Sci Eng 60:284–293. Scholar
  21. Svarovskaya LI, Filatov DA, Gerelmaa T et al (2009) IR and1H NMR assessments of the biodegradation of oil. Pet Chem 49(2):136–141CrossRefGoogle Scholar
  22. Svarovskaya LI, Altunina LK, Razd’yakonova GI et al (2016) Carbon sorbents for the development of biotechnologies. Int Polymer Sci Technol 43(4):15–18CrossRefGoogle Scholar
  23. Svarovskaya LI, Serebrennikova OV, Duchko MA (2017) Changes in the composition of the bituminous components of valley peat under stimulated microbial action. Solid Fuel Chem 51(2):67–77. Scholar
  24. Svarovskaya LI, Manakov AYu, Alltunina LK (2018) Nucleation and formation of gas hydrates depending on the physical and chemical properties of oils. Sib J life Sci Agric 10(1):64–74Google Scholar
  25. Turner DJ, Miller KT, Sloan ED (2009) Methane hydrate formation and an inward growing shell model in water-in-oil dispersions. Chem Eng Sci 64(18):3996–4004. Scholar
  26. Vater J, Kablitz B, Wilde C (2002) Matrix assisted laser desorption ionization-time of flight mass spectrometry lipopeptide biosurfactants in whole cells and culture extracts of Bacillus subtilis C-1 isolated from petroleum sludge. Appl Environ Microbiol 68(12):6210–6219. Scholar

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Lidia I. Svarovskaya
    • 1
    Email author
  • Andrey Y. Manakov
    • 2
  • Lyubov K. Altunina
    • 1
  • Larisa A. Strelets
    • 1
  1. 1.Institute of Petroleum Chemistry SB RASTomskRussia
  2. 2.Institute of Inorganic Chemistry SB RASNovosibirskRussia

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