Utilization of α-Glucosidic Disaccharides by Ogataea (Hansenula) polymorpha: Genes, Proteins, and Regulation
Utilization of α-glucosidic sugars such as maltose, maltotriose, isomaltose and sucrose has been extensively studied in a conventional yeast Saccharomyces cerevisiae as many important processes such as baking, brewing, and bioethanol production rely on fermentation of these sugars. In 1998, a non-conventional yeast Ogataea (formerly Hansenula) polymorpha was reported to grow on α-glucosidic disaccharides maltose and sucrose using intracellular α-glucosidase for their hydrolysis. Later on, the list of α-glucosidic sugars assimilated by O. polymorpha and hydrolyzed by its α-glucosidase was extended by adding maltotriose, isomaltose, palatinose, maltulose, and some others. In this chapter, we review the data on genetics, genomics, transport, and intracellular hydrolysis of α-glucosidic sugars in O. polymorpha. We also address evolution of yeast α-glucosidases and regulation of α-glucosidase and permease genes. Relevant data on other yeasts, mostly on S. cerevisiae, are used for comparison.
KeywordsMaltose Methylotrophic yeast Sugar transport Gene cluster Genome mining
This book chapter is based on experimental work supported by grants from the Estonian Research Council (ETF 3923, ETF 5676, ETF 7528; ETF 9072 and PUT1050).
- Ávila J, González C, Brito N et al (2002) A second Zn(II)2Cys6 transcriptional factor encoded by the YNA2 gene is indispensable for the transcriptional activation of the genes involved in nitrate assimilation in the yeast Hansenula polymorpha. Yeast 19:537–544. https://doi.org/10.1002/yea.847PubMedCrossRefGoogle Scholar
- Cihan A, Ozcan B, Tekin N, Cokmus C (2011) Characterization of a thermostable α-glucosidase from Geobacillus thermodenitrificans F84a, pp 945–955Google Scholar
- Janecek S (2009) Amylolytic enzymes-focus on the alpha-amylases from archaea and plants. Nova Biotechnol 9Google Scholar
- Krakenaĭte RP, Glemzha AA (1983) Some properties of two forms of alpha-glucosidase from Saccharomyces cerevisiae-II. Biokhimiia Mosc Russ 48:62–68Google Scholar
- Kramarenko T, Karp H, Järviste A, Alamäe T (2000) Sugar repression in the methylotrophic yeast Hansenula polymorpha studied by using hexokinase-negative, glucokinase-negative and double kinase-negative mutants. Folia Microbiol (Praha) 45:521–529. https://doi.org/10.1007/BF02818721CrossRefGoogle Scholar
- Limtong S, Srisuk N, Yongmanitchai W et al (2008) Ogataea chonburiensis sp. nov. and Ogataea nakhonphanomensis sp. nov., thermotolerant, methylotrophic yeast species isolated in Thailand, and transfer of Pichia siamensis and Pichia thermomethanolica to the genus Ogataea. Int J Syst Evol Microbiol 58:302–307. https://doi.org/10.1099/ijs.0.65380-0PubMedCrossRefGoogle Scholar
- Suppi S, Michelson T, Viigand K, Alamäe T (2013) Repression vs. activation of MOX, FMD, MPP1 and MAL1 promoters by sugars in Hansenula polymorpha: the outcome depends on cell’s ability to phosphorylate sugar. FEMS Yeast Res 13:219–232. https://doi.org/10.1111/1567-1364.12023PubMedCrossRefGoogle Scholar
- Viigand K (2018) Utilization of α-glucosidic sugars by Ogataea (Hansenula) polymorpha. Dissertation, University of Tartu. http://hdl.handle.net/10062/61743
- Viigand K, Tammus K, Alamäe T (2005) Clustering of MAL genes in Hansenula polymorpha: cloning of the maltose permease gene and expression from the divergent intergenic region between the maltose permease and maltase genes. FEMS Yeast Res 5:1019–1028. https://doi.org/10.1016/j.femsyr.2005.06.003PubMedCrossRefGoogle Scholar
- Visnapuu T, Mäe A, Alamäe T (2008) Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato. Process Biochem 43:414–422. https://doi.org/10.1016/j.procbio.2008.01.002CrossRefGoogle Scholar
- Zimmermann FK, Entian K-D (1997) Yeast Sugar Metabolism. CRC Press, Boca RatonGoogle Scholar