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Experimental Approaches for Genome Sequencing

  • Mohd Sayeed Akhtar
  • Ibrahim A. Alaraidh
  • Khalid Rehman Hakeem
Chapter

Abstract

Genome analysis is gradually used to tackle the problems related to the diagnosis, profiling of microbiome, genotyping, mutation, and evolutionary studies. A number of challenges associated with genome analysis are related to the sequencing approaches and the adopted methodologies. Therefore, availability of genome sequences is directly influenced by improvising the sequencing technology, and the use of modern technologies makes the sequencing cost-effective. Thus, the aim of this chapter is to provide the in-depth knowledge of various experimental approaches used for sequencing of the genome.

Keywords

BAC to BAC approach cDNA Genome PCR Shotgun approach 

Notes

Acknowledgments

The authors (Mohd Sayeed Akhtar and Ibrahim A. Alaraidh) are highly grateful to the Department of Botany, Gandhi Faiz-e-Aam College, Shahajahanpur, U.P., India, and the Botany and Microbiology Department, Science College, King Saud University, Riyadh, Kingdom of Saudi Arabia.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mohd Sayeed Akhtar
    • 1
  • Ibrahim A. Alaraidh
    • 2
  • Khalid Rehman Hakeem
    • 3
    • 4
  1. 1.Department of BotanyGandhi Faiz-e-Aam CollegeShahjahanpurIndia
  2. 2.Botany and Microbiology DepartmentKing Saud University, Science CollegeRiyadhSaudi Arabia
  3. 3.Department of Biological SciencesKing Abdulaziz UniversityJeddahSaudi Arabia
  4. 4.Princess Dr. Najla Bint Saud Al-Saud Center for Excellence Research in BiotechnologyKing Abdulaziz UniversityJeddahSaudi Arabia

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