Integration of “Omic” Approaches to Unravel the Heavy Metal Tolerance in Plants
Recent adverse climatic changes have limited crop productivity, which in turn causes food crises throughout the globe, but this is more concerning for developing countries. Interest for practical genomics as well as proteomics is currently widespread to understand the mechanism(s) of stress tolerance. The information gained through the said methods would facilitate the nutritional management and metabolic and genetic improvement of plants of our interest. Genomic and proteomic studies of control and stressed plants have empowered the recognizable proof of various traits and proteins that assume vital part in granting stress resistance to plants. Therefore, identification of “stress-responsive” protein(s)/gene(s) and utilization of thus obtained data provide a great chance to reduce effects of any particular or combined stress on plant. On the other hand, it is also necessary to investigate the cross talk between key stress reaction pathways/components in order to better understand the regulation of protection mechanism. This review provides a critical and extensive analysis of the proteomic profiling of plants in reply to heavy metal stress and highlights key techniques being employed for accomplishing ever-desired success. It also revealed an account of proteins identified to impart resistance under different abiotic stresses in plants. In addition, we present the role of functional genomics and various bioinformatics tools to understand the proteome maps of heavy metal-stressed plants.
KeywordsHeavy metal stress Proteomics Functional genomics Bioinformatics
- FAO (2012) Statistical yearbook Viale delle Terme di Caracalla. Rome ISBN 978-92-5-107083-3Google Scholar
- GenomeWeb News (2001) Backed by Compaq, Bruker, and Novartis, Gene-Prot opens industrial-scale proteomics facility. 26 April (www.genomeweb.com)Google Scholar
- Hajduch M, Rakwal R, Agrawal GK, Yonekura M, Pretova A (2001) High-resolution two-dimensional electrophoresis separation of proteins from metal-stressed rice (Oryza sativa L.) leaves: drastic reductions/fragmentation of ribulose-1,5-bisphosphatecarboxylase/oxygenase and induction of stress-related proteins. Electrophoresis 22:2824–2831CrossRefPubMedGoogle Scholar
- Shin DH, Kamal AHM, Suzuki T, Yun YH, Lee MS, Chung KY, Jeong HS, Park CH, Choi JS, Woo SH (2010) Reference proteome map of buckwheat (Fagopyrum esculentum and Fagopyrum tataricum) leaf and stem cultured under light or dark. Aust J Crop Sci 4(8):633–641Google Scholar
- Zhu J, Gong Z, Zhang C, Song CP, Damsz B, Inan G, Koiwa H, Zhu JK, Hasegawa PM, Bressan RA (2002) OSM1/SYP61 a syntaxin protein in arabidopsis controls abscisic acid–mediated and non-abscisic acid–mediated responses to abiotic stress. Plant Cell 14(12):3009–3028CrossRefPubMedPubMedCentralGoogle Scholar