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Toxicity of Azo Dyes in Pharmaceutical Industry

  • Armina GičevićEmail author
  • Lamija Hindija
  • Alma Karačić
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 73)

Abstract

Azo compounds represent about two thirds of all synthetic dyes. Their usage in pharmaceutical industry has many purposes. One of the most important is coloring of pharmaceutical agents which improves their easy identification. Azo dyes often used in manufacturing of pharmaceuticals are: E102 Tartrazine, E110 Sunset Yellow FCF, Ponceau 4R (Cochineal Red A), Azorubine (Carmoisine), Amaranth, E133 Brilliant Blue and E129 Allura Red. Many azo dyes show carcinogenic and mutagenic activity, and they can provoke allergic reactions. Generally, toxicity of ingredients grows with the increase of benzene rings in their structure. Carcinogenicity of azo dyes directly depends on the structure of molecule and on mechanism of degradation. Products of degradation of azo dyes are mostly aromatic amines with different structures and they can also have carcinogenic properties. Carcinogenicity of many azo dyes is due to their cleaved products such as benzidine. Benzidine is known as carcinogen for the human urinary bladder. Except of carcinogenic and mutagenic activity, azo dyes can alter biochemical markers and they can provoke allergic reactions.

Keywords

Azo dyes Toxicity Carcinogenicity Allergic reactions Pharmaceutical industry 

Notes

Conflict of Interest

The authors have no conflicts of interest to disclose.

Reference

  1. 1.
    Šuleková, M., Smrčová, M., Hudák, A., Heželová, M., Fedorová, M.: Organic colouring agents in the pharmaceutical industry. Folia Vet. 61(3), 32–46 (2017)CrossRefGoogle Scholar
  2. 2.
    Pawar, S., Kumar, A.: Issues in the formulation of drugs for oral use in children: role of excipients. Paediatr. Drugs 4(6), 371–379 (2002)CrossRefGoogle Scholar
  3. 3.
    Chung, K.T.: Azo dyes and human health: a review. J. Environ. Sci. Health C Environ. Carcinog. Ecotoxicol. Rev. 34(4), 233–261 (2016)CrossRefGoogle Scholar
  4. 4.
    Feng, J., Cerniglia, C.E., Chen, H.: Toxicological significance of azo dye metabolism by human intestinal microbiota. Front Biosci. (Elite ed.) 4, 568–586 (2012)CrossRefGoogle Scholar
  5. 5.
    Floriano, J.M., da Rosa, E., do Amaral, Q.D.F., Zuravski, L., Chaves, P.E.E., Machado, M.M., et al.: Is tartrazine really safe? In silico and ex vivo toxicological studies in human leukocytes: a question of dose. Toxicol Res. (Camb.) 7(6), 1128–1134 (2018)CrossRefGoogle Scholar
  6. 6.
    Gao, Y., Li, C., Shen, J., Yin, H., An, X., Jin, H.: Effect od food azo dye tartrazine on learning and memory functions in mice and rats, and the possible mechanisms involved. J. Food Sci. 76(6), 125–129 (2011)CrossRefGoogle Scholar
  7. 7.
    Khayyat, L.I., Essawy, A.E., Sorour, J.M., Soffar, A.: Sunset yellow and Allura red modulate Bcl2 and COX2 expression levels and confer oxidative stress-mediated renal and hepatic toxicity in male rats. PeerJ. 6, e5689 (2018)CrossRefGoogle Scholar
  8. 8.
    Qu, D., Gu, Y., Feng, L., Han, J.: High content analysis technology for evaluating the joint toxicity of sunset yellow and sodium sulfite in vitro. Food Chem. 233, 135–143 (2017)CrossRefGoogle Scholar
  9. 9.
    Ali, M.Y., Hassan, G.M., Hassan, A.M.S., Mohamed, Z.A., Ramadan, M.F.: In vivo genotoxicity assessment of sunset yellow and sodium benzoate in female rats. Drug Chem. Toxicol, 1–10 (2018)Google Scholar
  10. 10.
    Haverić, A., Haverić, S., Hadžić, M., Lojo-Kadrić, N., Ibrulj, S.: Genotoxicity and cytotoxicity analysis of curcumin and sunset yellow in human lymphocyte culture. Cell. Mol. Biol. (Noisy-le-grand) 64(3), 87–91 (2018)CrossRefGoogle Scholar
  11. 11.
    Amin, K.A., Abdel Hameid, H., Abd Elsttar, A.H.: Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem. Toxicol. 48(10), 2994–2999 (2010)CrossRefGoogle Scholar
  12. 12.
    Cemek, M., Büyükokuroğlu, M.E., Sertkaya, F., Alpdağtaş, S., Hazini, A., Önül, A., et al.: Effects of food colour additives on antioxidant functions and bioelement contents of liver, kidney and brain tissues in rats. J. Food Nutr. 2(10), 686–691 (2014)Google Scholar
  13. 13.
    Rowe, R.C., Sheskey, P.J., Quinn, M.E.: Handbook of Pharmaceutical Excipients, 6th edn. Pharmaceutical Press and American Pharmacists Association, USA (2009)Google Scholar
  14. 14.
    EFSA: Scientific opinion on the re-evaluation of Azorubine/Carmoisine (E 122) as a food additive. https://www.efsa.europa.eu/en/efsajournal/pub/1332. Accessed 12 Dec 2018
  15. 15.
    Uysal, H., Genc, S., Ayar, A.: Toxic effects of chronic feeding with food azo dyes on drosophila melanogaster oregon R. Sci. Iran. C 24(6), 3081–3086 (2017)Google Scholar
  16. 16.
    Remy, M., Thaler, S., Schumann, R.G., May, C.A., Fiedorowicz, M., Schuettauf, F., et al.: An in vivo evaluation of brilliant blue G in animals and humans. Br. J. Ophthalmol. 92, 1142–1147 (2008)CrossRefGoogle Scholar
  17. 17.
    Bastaki, M., Farrel, T., Bhusari, S., Pant, K., Kulkarni, R.: Lack of genotoxicity in vivo for food colour additive allura red AC. Food Chem. Toxicol. 105, 308–314 (2017)CrossRefGoogle Scholar
  18. 18.
    Rajan, A.P., Anandan, S.: Investigation of carcinogenic and mutagenic property of food colour using catfish Clarias Batrachus by using alkaline single-cell gel electrophoresis (comet) assay and micronucleus assay. Int. J. Med. Res. Pharmacol. Sci. 4(7), 29–34 (2017)Google Scholar

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Armina Gičević
    • 1
    Email author
  • Lamija Hindija
    • 2
  • Alma Karačić
    • 3
  1. 1.Department of Pharmaceutical Analysis, Faculty of PharmacyUniversity of SarajevoSarajevoBosnia and Herzegovina
  2. 2.Department of Pharmaceutical Technology, Faculty of PharmacyUniversity of SarajevoSarajevoBosnia and Herzegovina
  3. 3.SarajevoBosnia and Herzegovina

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