Nanoemulsions: A Promising Tool for Dairy Sector

  • Anil Panghal
  • Navnidhi Chhikara
  • V. Anshid
  • Manga Veera Sai Charan
  • Vinod Surendran
  • Anju Malik
  • Sanju Bala Dhull
Part of the Nanotechnology in the Life Sciences book series (NALIS)


Nanoemulsions are the emulsions having droplet size below 100 nm developed by adopting pressure- or energy-based methods like homogenizers with a high-pressure valve or using microfluidizers. Within the droplets, the combination of functional food components is possible with the continuous or interfacial region. It provides the ability to encapsulate and release at a single delivering system. Such systems can carry numerous functional components, and the release of these components is controlled with a particular environmental trigger. In milk and milk-based products, various organoleptic characteristics like mouthfeel, taste, flavor, consistency, and rheological characteristics are considered as the quality determinants. The achievement of desired quality parameters in a product can be done by controlling the distribution and droplet size assisted by emulsification process. The stabilizing ability of emulsion in milk is possible without using any extraneous stabilizing agents due to inherent emulsifying capacity of milk proteins. The demand for nanoemulsions over conventional emulsions is increasing day by day, and research is emphasized on their particular applications and properties like functional beverages and foods, improved bioavailability of nutrients, and enhanced physical stability. The dairy-based products can be used as ingredients which are having different functions that supply physical stability as well as health and nutritional benefits. This chapter is focused on the nanoemulsion formation and their applications in dairy-based industries.


Emulsion Nanoemulsion Dairy products Dairy sector Milk 


  1. Aditya NP, Macedo AS, Doktorovova S, Souto EB, Kim S, Chang PS, Ko S (2014) Development and evaluation of lipid nanocarriers for quercetin delivery: a comparative study of solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC), and lipid nanoemulsions (LNE). LWT Food Sci Technol 59(1):115–121CrossRefGoogle Scholar
  2. Bhushan B (ed) (2017) Springer handbook of nanotechnology. Springer, Berlin/HeidelbergGoogle Scholar
  3. Cavazos-Garduño A, Flores AO, Serrano-Niño JC, Martínez-Sanchez CE, Beristain CI, García HS (2015) Preparation of betulinic acid nanoemulsions stabilized by ω-3 enriched phosphatidylcholine. Ultrason Sonochem 24:204–213PubMedCrossRefGoogle Scholar
  4. Cha DS, Chinnan MS (2004) Biopolymer-based antimicrobial packaging: a review. Crit Rev Food Sci Nutr 44(4):223–237PubMedCrossRefGoogle Scholar
  5. Charcosset C (2016) Preparation of nanomaterials for food applications using membrane emulsification and membrane mixing. In: Nanotechnology in the Agri-Food Industry, Emulsions by Grumezescu AM, Academic Press, Vol. 3, pp 37-69. Scholar
  6. Corredig M (2009) Molecular understanding of the interaction of dairy proteins with other food biopolymers. In: Dairy-derived ingredients. Woodhead Publishing, Cambridge, pp 371–393CrossRefGoogle Scholar
  7. Dewettinck K, Rombaut R, Thienpont N, Le TT, Messens K, Van Camp J (2008) Nutritional and technological aspects of milk fat globule membrane material. Int Dairy J 18(5):436–457CrossRefGoogle Scholar
  8. Dickinson E (2003) Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocoll 17(1):25–39CrossRefGoogle Scholar
  9. Donsì F, Annunziata M, Sessa M, Ferrari G (2011) Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT Food Sci Technol 44(9):1908–1914CrossRefGoogle Scholar
  10. Frestedt JL, Zenk JL, Kuskowski MA, Ward LS, Bastian ED (2008) A whey-protein supplement increases fat loss and spares lean muscle in obese subjects: a randomized human clinical study. Nutr Metab 5:8CrossRefGoogle Scholar
  11. Ghosh V, Mukherjee A, Chandrasekaran N (2013) Ultrasonic emulsification of food-grade nanoemulsion formulation and evaluation of its bactericidal activity. Ultrason Sonochem 20(1):338–344PubMedPubMedCentralCrossRefGoogle Scholar
  12. Gruenwald J (2009) Novel botanical ingredients for beverages. Clin Dermatol 27(2):210–216PubMedCrossRefGoogle Scholar
  13. Helmut Kaiser Consultancy (2009) Study: nanobased products inventory 2009 and commercialization. Accessed 15 June 2018.Google Scholar
  14. Kelmann RG, Kuminek G, Teixeira HF, Koester LS (2007) Carbamazepine parenteral nanoemulsions prepared by spontaneous emulsification process. Int J Pharm 342(1–2):231–239PubMedCrossRefGoogle Scholar
  15. Koroleva MY, Yurtov EV (2012) Nanoemulsions: the properties, methods of preparation and promising applications. Russ Chem Rev 81(1):21–43CrossRefGoogle Scholar
  16. Kuo F, Subramanian B, Kotyla T, Wilson TA, Yoganathan S, Nicolosi RJ (2008) Nanoemulsions of an anti-oxidant synergy formulation containing gamma tocopherol have enhanced bioavailability and anti-inflammatory properties. Int J Pharm 363(1–2):206–213PubMedCrossRefGoogle Scholar
  17. Laouini A, Fessi H, Charcosset C (2012) Membrane emulsification: a promising alternative for vitamin E encapsulation within nano-emulsion. J Membr Sci 423:85–96CrossRefGoogle Scholar
  18. Li PH, Chiang BH (2012) Process optimization and stability of D-limonene-in-water nanoemulsions prepared by ultrasonic emulsification using response surface methodology. Ultrason Sonochem 19(1):192–197PubMedCrossRefGoogle Scholar
  19. Liedtke S, Wissing S, Müller RH, Mäder K (2000) Influence of high pressure homogenisation equipment on nanodispersions characteristics. Int J Pharm 196(2):183–185PubMedCrossRefGoogle Scholar
  20. McClements DJ (2012) Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter 8(6):1719–1729CrossRefGoogle Scholar
  21. Noh SK, Koo SI (2004) Milk sphingomyelin is more effective than egg sphingomyelin in inhibiting intestinal absorption of cholesterol and fat in rats. J Nutr 134(10):2611–2616PubMedCrossRefGoogle Scholar
  22. Oh DH, Balakrishnan P, Oh YK, Kim DD, Yong CS, Choi HG (2011) Effect of process parameters on nanoemulsion droplet size and distribution in SPG membrane emulsification. Int J Pharm 404(1–2):191–197PubMedCrossRefGoogle Scholar
  23. Parris N, Purcell JM, Ptashkin SM (1991) Thermal denaturation of whey proteins in skim milk. J Agric Food Chem 39(12):2167–2170CrossRefGoogle Scholar
  24. Preetz C, Hauser A, Hause G, Kramer A, Mäder K (2010) Application of atomic force microscopy and ultrasonic resonator technology on nanoscale: distinction of nanoemulsions from nanocapsules. Eur J Pharm Sci 39(1–3):141–151PubMedCrossRefGoogle Scholar
  25. Qian C, McClements DJ (2011) Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: factors affecting particle size. Food Hydrocoll 25(5):1000–1008CrossRefGoogle Scholar
  26. Ribeiro Filho HM, Delagarde R, Peyraud JL (2005) Herbage intake and milk yield of dairy cows grazing perennial ryegrass swards or white clover/perennial ryegrass swards at low-and medium-herbage allowances. Anim Feed Sci Technol 119(1–2):13–27CrossRefGoogle Scholar
  27. Saberi AH, Fang Y, McClements DJ (2013) Fabrication of vitamin E-enriched nanoemulsions by spontaneous emulsification: effect of propylene glycol and ethanol on formation, stability, and properties. Food Res Int 54(1):812–820CrossRefGoogle Scholar
  28. Schokker EP, Singh H, Creamer LK (2000) Heat-induced aggregation of β-lactoglobulin A and B with α-lactalbumin. Int Dairy J 10(12):843–853CrossRefGoogle Scholar
  29. Solè I, Pey CM, Maestro A, González C, Porras M, Solans C, Gutiérrez JM (2010) Nano-emulsions prepared by the phase inversion composition method: Preparation variables and scale up. J Colloid Interface Sci 344(2):417–423PubMedCrossRefGoogle Scholar
  30. Sonneville-Aubrun O, Babayan D, Bordeaux D, Lindner P, Rata G, Cabane B (2009) Phase transition pathways for the production of 100 nm oil-in-water emulsions. Phys Chem Chem Phys 11(1):101–110PubMedCrossRefGoogle Scholar
  31. Strunz CC, Oliveira TV, Vinagre JC, Lima A, Cozzolino S, Maranhão RC (2008) Brazil nut ingestion increased plasma selenium but had minimal effects on lipids, apolipoproteins, and high-density lipoprotein function in human subjects. Nutr Res 28(3):151–155PubMedCrossRefGoogle Scholar
  32. Subramanian B, Kuo F, Ada E, Kotyla T, Wilson T, Yoganathan S, Nicolosi R (2008) Enhancement of anti-inflammatory property of aspirin in mice by a nano-emulsion preparation. Int Immunopharmacol 8(11):1533–1539PubMedCrossRefGoogle Scholar
  33. Tang SY, Shridharan P, Sivakumar M (2013) Impact of process parameters in the generation of novel aspirin nanoemulsions–comparative studies between ultrasound cavitation and microfluidizer. Ultrason Sonochem 20(1):485–497PubMedCrossRefGoogle Scholar
  34. Wilde PJ (2009) Emulsions and nanoemulsions using dairy ingredients. In: Dairy-derived ingredients by Corredig, M, Woodhead Publishing,Cambridge, UK, pp 539–564.CrossRefGoogle Scholar
  35. Yilmaz E, Borchert HH (2005) Design of a phytosphingosine-containing, positively-charged nanoemulsion as a colloidal carrier system for dermal application of ceramides. Eur J Pharm Biopharm 60(1):91–98PubMedCrossRefGoogle Scholar
  36. Yuan Y, Gao Y, Zhao J, Mao L (2008) Characterization and stability evaluation of β-carotene nanoemulsions prepared by high pressure homogenization under various emulsifying conditions. Food Res Int 41(1):61–68CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Anil Panghal
    • 1
  • Navnidhi Chhikara
    • 1
  • V. Anshid
    • 1
  • Manga Veera Sai Charan
    • 1
  • Vinod Surendran
    • 1
  • Anju Malik
    • 2
  • Sanju Bala Dhull
    • 3
  1. 1.Department of Food Technology and NutritionLovely Professional UniversityJalandharIndia
  2. 2.Department of Energy and Environmental SciencesChaudhary Devi Lal UniversitySirsaIndia
  3. 3.Department of Food Science and TechnologyChaudhary Devi Lal UniversitySirsaIndia

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