Micro-CT in Artificial Tissues

  • Leyla Türker ŞenerEmail author
  • Gürcan Albeniz
  • Göker Külüşlü
  • Işil Albeniz


The computed tomography (CT) technology, whose theoretical basis goes back to 1917, started after an Australian mathematician Johan Radon proved that the reconstruction of an (N) dimensional object might be obtained from the (N-1) dimensional projections of the same object. As the general structure, the micro-CT (microcomputed tomography) is similar to computed tomography. Computed tomography was invented in 1972 by engineer Godfrey Hounsfield and physician Allan Cormack. Hounsfield invented the first whole-body computed tomography device in 1975. Hounsfield and Allan McLeod Cormack were awarded with a Nobel Prize in 1979 for their studies on X-ray-based computed tomography and diagnostic techniques.


Micro-CT Artificial tissues Scaffolds 3D bioprinting Computed tomography Computer-assisted design Computer-assisted manufacturing (CAD/CAM) Magnetic resonance imaging (MRI) 


  1. 1.
    Panchbhai SA. Wilhelm conrad röntgen and the discovery of X-rays: revisited after centennial. J Indian Acad Oral Med Radiol. 2015;27(1):90–5.CrossRefGoogle Scholar
  2. 2.
    Plessis A, Broeckhoven C, Guelpa A, Gerhard le Roux S. Laboratory x-ray micro-computed tomography: a user guideline for biological samples. GigaScience. 2017;6(6):1–11.CrossRefGoogle Scholar
  3. 3.
    Bhattacharyya KB. Godfrey Newbold Hounsfield (1919–2004): the man who revolutionized neuroimaging. Ann Indian Acad Neurol. 2016;19(4):448–50. Scholar
  4. 4.
    Keleş A, Alçin H, Kamalak A, Versiani MA. Micro-CT evaluation of root filling quality in oval-shaped canals. Int Endod J. 2014;47(12):1177–84.CrossRefGoogle Scholar
  5. 5.
    Khalil S, Sun W. Bioprinting endothelial cells with alginate for 3D tissue constructs. J Biomech Eng. 2009;131:111002–8.CrossRefGoogle Scholar
  6. 6.
    Yao R, Zhang R, Luan J, Lin F. Alginate and alginate/gelatin microspheres for human adipose derived stem cell encapsulation and differentiation. Biofabrication. 2012;4(2):025007.CrossRefGoogle Scholar
  7. 7.
    Zhang Y, Chen H, Ozbolat IT. Characterization of printable micro-fluidic channels for organ printing. Texas: International Mechanical Engineering Congress & Exposition Houston; 2012.CrossRefGoogle Scholar
  8. 8.
    Langer R, Vacanti JP. Tissue engineering. Science. 1993;60:920–6.CrossRefGoogle Scholar
  9. 9.
    Hollister SJ. Porous scaffold design for tissue engineering. Nat Mater. 2005;4(7):518–24.CrossRefGoogle Scholar
  10. 10.
    Kim BS, Park IK, Hoshiba T, Jiang HL, Choi YJ, Akaike T, Cho CS. Design of artificial extracellular matrices for tissue engineering. Prog Polym Sci. 2011;36:238–68.CrossRefGoogle Scholar
  11. 11.
    Ma Z, Kotaki M, Yong T, He W, Ramakrishna S. Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering. Biomaterials. 2005;26(15):2527–36.CrossRefGoogle Scholar
  12. 12.
    Mann BK, West JL. Cell adhesion peptides alter smooth muscle cell adhesion, proliferation, migration, and matrix protein synthesis on modified surfaces and in polymer scaffolds. J Biomed Mater Res. 2002;60(1):86–93.CrossRefGoogle Scholar
  13. 13.
    Berry CC, Campbell G, Spadaccino A, Robertson M, Curtis AS. The influence of microscale topography on fibroblast attachment and motility. Biomaterials. 2004;25(26):5781–8.CrossRefGoogle Scholar
  14. 14.
    Wan Y, Wang Y, Liu Z, Qu X, Han B, Bei J, Wang S. Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly (L-lactide). Biomaterials. 2005;26(21):4453.CrossRefGoogle Scholar
  15. 15.
    Lam CXF, Mo XM, Teoh SH, Hutmacher DW. Scaffold development using 3D printing with a starch-based polymer. Mater Sci Eng C. 2002;20(1/2):49–56.CrossRefGoogle Scholar
  16. 16.
    An J, Ee Mei Teoh J, Suntornnond R, Kai Chua C. Design and 3D printing of scaffolds and tissues. Engineering. 2015;1(2):261–8.CrossRefGoogle Scholar
  17. 17.
    Ma Z, Kotaki M, Inai R, Ramakrishna S. Potential of nanofiber matrix as tissue-engineering scaffolds. Tissue Eng. 2005;11(1–2):101–9.CrossRefGoogle Scholar
  18. 18.
    He P, Zhao J, Zhang J, Li B, Gou Z, Gou M, Xi L. Bioprinting of skin constructs for wound healing. Burns Trauma. 2018;6:5.CrossRefGoogle Scholar
  19. 19.
    Lanza R, Langer R, Vacanti J. Principles of tissue engineering. 3rd ed. Cambridge: Academic Press; 1997. eBook ISBN: 9780123983701Google Scholar
  20. 20.
    Singh D, Singh D, Han SS. 3D printing of scaffold for cells delivery: advances in skin tissue engineering. Polymers. 2016;8(1):19.CrossRefGoogle Scholar

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© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • Leyla Türker Şener
    • 1
    Email author
  • Gürcan Albeniz
    • 2
  • Göker Külüşlü
    • 3
  • Işil Albeniz
    • 1
  1. 1.Istanbul Faculty of Medicine, Department of BiophysicsIstanbul UniversityIstanbulTurkey
  2. 2.Cerrahpaşa Faculty of Medicine, Department of General SurgeryIstanbul University CerrahpaşaIstanbulTurkey
  3. 3.Istanbul Faculty of Medicine, 3B Medical and Industrial Design LaboratoryIstanbul UniversityIstanbulTurkey

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