In experimental neurologic injury models, evaluating the effect of any manipulation or treatment on cerebral blood flow (CBF) gives significant information about metabolic changes in rodent’s brains. There are different methods of investigating brain microcirculation based on changes of H2 clearance, radionuclide labeled microspheres, pial artery diameter, and iodoantipyrine. Another method, CBF measurement using laser Doppler, is based on measurements of absolute changes in regional blood flow of the cortex and detects real time changes in microvascular perfusion in a noninvasive manner. In this chapter we will approach the brain monitoring mechanism with laser Doppler equipment in a step-by-step fashion. We will demonstrate the potential pitfalls and how to avoid them. A rat model will be used to demonstrate brain monitoring and the associated procedures that accompany the process.
KeywordsBrain monitoring Syncope Animal models Brain imaging Cerebral blood flow
A special thank you to Devin McBride for helping set up the equipment for the CBF pictures.
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