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Rodent Spinal Cord Demyelination Models

  • Kariena R. Andres
  • Johnny R. Morehouse
  • Rachel Cary
  • Christine D. Yarberry
  • Nicholas J. Kuypers
  • Scott R. WhittemoreEmail author
Chapter
Part of the Springer Series in Translational Stroke Research book series (SSTSR)

Abstract

Oligodendrocyte loss and subsequent demyelination is a significant component of the demyelinating diseases such as multiple sclerosis (MS) and traumatic CNS injury such as spinal cord (SCI) or traumatic brain (TBI) injury. Therefore, remyelination, either by enhancing endogenous myelination or engrafting exogenous myelinating cells, is a viable therapeutic target to restore function. To assess specific approaches to facilitate functional remyelination in vivo, appropriate injury models are needed. This chapter will discuss the strengths and weaknesses of a number of demyelinating lesions of the spinal cord and provide guidelines for choosing which model best suits which experimental condition. Step by step procedures for both creating and assessing the lesion will be provided.

Keywords

Spinal cord injury Demyelination Ethidium bromide Lysolecithin Cuprizone 

Notes

Acknowledgements

Supported by RR15576/GM103507, NS054708, Norton Healthcare, Commonwealth of Kentucky Challenge for Excellence, and the Kentucky Spinal Cord and Head Injury Research Trust.

Supplementary material

Video 1

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Kariena R. Andres
    • 1
    • 2
  • Johnny R. Morehouse
    • 1
    • 2
  • Rachel Cary
    • 1
    • 2
  • Christine D. Yarberry
    • 1
    • 2
  • Nicholas J. Kuypers
    • 1
    • 2
    • 3
  • Scott R. Whittemore
    • 1
    • 2
    • 3
    Email author
  1. 1.Kentucky Spinal Cord Injury Research CenterUniversity of Louisville School of MedicineLouisvilleUSA
  2. 2.Department of Neurological SurgeryUniversity of Louisville School of MedicineLouisvilleUSA
  3. 3.Department of Anatomical Sciences and NeurobiologyUniversity of Louisville School of MedicineLouisvilleUSA

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