Method of Spinal Cord Contusion Injury Created by Tissue Displacement
Experimental contusion spinal cord injury (SCI) is the most frequently used in vivo model to provide insight into the mechanisms of secondary injury. Many criteria have been utilized to control the severity of SCI, including the height of the weight drop, the magnitude of the force striking the spinal cord, and the degree of spinal cord tissue displacement. The tissue displacement method is the most reliable technique of producing experimental SCI after the target spine has been stabilized. We have described the creation of a contusion SCI model using the LISA that is versatile and may be adapted for use in mice, rats, and monkeys with minor adjustments in the mechanical settings. Reproducible and reliable contusion SCI may be produced using this method of tissue displacement that may be characterized by gradable behavioral and morphological assessment.
KeywordsSpinal cord injury Contusion SCI model Louisville Injury System Apparatus (LISA) Spinal cord tissue displacement injury
We acknowledge Norton Healthcare for their continued support.
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