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Rodent Surgical Procedures and Tissue Collection

  • Cesar Reis
  • Onat Akyol
  • Jay Malaguit
  • Lei Huang
  • Budbazar Enkhjargal
  • Richard ApplegateII
  • Robert Martin
  • Gary Stier
  • John Zhang
Chapter
Part of the Springer Series in Translational Stroke Research book series (SSTSR)

Abstract

Collection of tissue for neurological investigation requires training and development of skills to assure precision in the sampling of not only the brain tissues but also other organs associated with each experiment. Preparation for sample analysis follows successful collection. A diverse amount of animal models have been successfully used for investigation of new pathways or specific diseases that may someday lead to improvement of future medicine. For that reason, tissue collection is extremely important to be mastered to achieve appropriate data results. In this chapter, we will cover steps to intubate, perfuse, remove, and separate the brain into anatomical sections as well as specific staining of the brain with triphenyltetrazolium chloride (TCC).

Keywords

Rodent model Ventilation Tracheostomy Rodent model Intubation Brain Perfusion Fixation Brain dissection Triphenyltetrazolium chloride (TCC) staining 

Notes

Acknowledgments

Thank you Dr. Weiwei Xu for providing the final TTC picture on Fig. 9.

References

  1. 1.
    Chen J, Xu X-M, Zhang JH. Animal models of acute neurological injuries. In: Zhang JH, editor. Animal models of acute neurological injuries. 1st ed. Totowa, NJ: Springer Science and Business Media; Humana Press; 2009.CrossRefGoogle Scholar
  2. 2.
    Jou IM, Tsai YT, Tsai CL, Wu MH, Chang HY, Wang NS. Simplified rat intubation using a new oropharyngeal intubation wedge. J Appl Physiol (1985). 2000;89(5):1766–70.CrossRefGoogle Scholar
  3. 3.
    Karwacki Z, Kowianski P, Morys J. General anaesthesia in rats undergoing experiments on the central nervous system. Folia Morphol (Warsz). 2001;60(4):235–42.Google Scholar
  4. 4.
    Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 2002;161(6):1961–71.CrossRefGoogle Scholar
  5. 5.
    Lee HB, Blaufox MD. Blood volume in the rat. J Nucl Med. 1985;26(1):72–6.PubMedGoogle Scholar
  6. 6.
    Ayer R, Cahill J, Sugawara T, Jadhav V, Zhang JH. Rodent surgical procedures and tissue collection. In: Chen J, Xu ZC, Xu X-M, Zhang JH, editors. Animal models of acute neurological injuries. Totowa, NJ: Humana Press; 2009. p. 19–38.CrossRefGoogle Scholar
  7. 7.
    Kramer M, Dang J, Baertling F, et al. TTC staining of damaged brain areas after MCA occlusion in the rat does not constrict quantitative gene and protein analyses. J Neurosci Methods. 2010;187(1):84–9.CrossRefGoogle Scholar
  8. 8.
    Benedek A, Moricz K, Juranyi Z, et al. Use of TTC staining for the evaluation of tissue injury in the early phases of reperfusion after focal cerebral ischemia in rats. Brain Res. 2006;1116(1):159–65.CrossRefGoogle Scholar
  9. 9.
    Joshi CN, Jain SK, Murthy PS. An optimized triphenyltetrazolium chloride method for identification of cerebral infarcts. Brain Res Brain Res Protoc. 2004;13(1):11–7.CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Cesar Reis
    • 1
    • 2
  • Onat Akyol
    • 2
  • Jay Malaguit
    • 2
  • Lei Huang
    • 1
    • 2
  • Budbazar Enkhjargal
    • 2
  • Richard ApplegateII
    • 3
    • 4
  • Robert Martin
    • 1
  • Gary Stier
    • 1
  • John Zhang
    • 1
    • 2
    • 5
    • 6
  1. 1.Department of AnesthesiologyLoma Linda University Medical CenterLoma LindaUSA
  2. 2.Department of Physiology and PharmacologyLoma Linda University School of MedicineLoma LindaUSA
  3. 3.Department of AnesthesiologyUC Davis School of MedicineSacramentoUSA
  4. 4.Department of Pain MedicineUC Davis School of MedicineSacramentoUSA
  5. 5.Department of AnesthesiologyLoma Linda University School of MedicineLoma LindaUSA
  6. 6.Department of NeurosurgeryLoma Linda University School of MedicineLoma LindaUSA

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