Rodent Surgical Procedures and Tissue Collection
Collection of tissue for neurological investigation requires training and development of skills to assure precision in the sampling of not only the brain tissues but also other organs associated with each experiment. Preparation for sample analysis follows successful collection. A diverse amount of animal models have been successfully used for investigation of new pathways or specific diseases that may someday lead to improvement of future medicine. For that reason, tissue collection is extremely important to be mastered to achieve appropriate data results. In this chapter, we will cover steps to intubate, perfuse, remove, and separate the brain into anatomical sections as well as specific staining of the brain with triphenyltetrazolium chloride (TCC).
KeywordsRodent model Ventilation Tracheostomy Rodent model Intubation Brain Perfusion Fixation Brain dissection Triphenyltetrazolium chloride (TCC) staining
Thank you Dr. Weiwei Xu for providing the final TTC picture on Fig. 9.
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