Collagenase Induced Pontine Hemorrhage in Rats

  • Loretta O. Iniaghe
  • Devin W. McBride
  • Tim Lekic
  • Jiping Tang
  • John H. Zhang
Part of the Springer Series in Translational Stroke Research book series (SSTSR)


Pontine hemorrhage is a devastating subtype of stroke with high rates of morbidity and mortality and currently has no treatment. Animal models are useful in elucidating the pathophysiological changes which occur in pontine hemorrhage and to evaluate potential therapeutic interventions. This chapter describes the bacterial collagenase injection in rats which creates a reproducible hematoma in the brain stem of rats. Detailed methods, an overview of the outcomes, and the limitations and alternatives is presented.


Pontine hemorrhage Collagenase Hematoma Rat model 

Supplementary material

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Loretta O. Iniaghe
    • 1
    • 2
  • Devin W. McBride
    • 1
  • Tim Lekic
    • 1
  • Jiping Tang
    • 1
  • John H. Zhang
    • 1
    • 3
  1. 1.Department of Physiology and PharmacologyLoma Linda University School of MedicineLoma LindaUSA
  2. 2.Department of Pharmacology and ToxicologyUniversity of BeninBeninNigeria
  3. 3.Department of AnesthesiologyLoma Linda University School of MedicineLoma LindaUSA

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