Recent Applications of Mass Spectrometry at Clarkson University

  • Madhuri Jayathirtha
  • Danielle Whitham
  • Sydney Stradtman
  • Costel C. DarieEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1140)


Mass spectrometry (MS) is a powerful technique that has various applications including the identification and characterization of proteins, protein-protein interactions and protein post translational modifications, as well as other molecules (i.e. metabolites, lipids, nucleotides and polynucleotides). However, not too many undergraduate students within the USA and around the world have access to (and are trained in) MS. The undergraduate students in our department are taught to analyze proteomics and metabolomics data obtained from MS analysis, including de novo sequencing of peptides and to interpret the MS and MS/MS data acquired in positive and negative ionization modes. Here, we give some examples of MS data analyzed in the Biochemistry I class and then examples of some independent research projects performed by students over the years in the Biochemistry and Biotechnology laboratory, where MS is used for both proteins, peptides and metabolites analysis, thus demonstrating the applicability of MS analysis in diverse fields. The projects discussed include analysis of the protein content present in yogurt, beer, protein shakes, contact lenses, or milk of animal or vegetal origin.


Mass spectrometry Proteomics Metabolomics Undergraduate students 



Bicinchoninic acid protein assay


Electron spray ionization


Matrix assisted laser desorption ionization


Mass spectrometry




Protein Lynx Global Server


Proteins migrating faster than albumin


Quadruple time of flight


Sodium dodecyl sulfate-poly acrylamide gel electrophoresis


Total ion chromatogram


Ultra performance liquid chromatography



We would like to thank all the students, who did their independent research projects in Biochemistry and Biotechnology lab course using MS method for analysis and other students in the lab for creating a pleasant environment. Part of this chapter was partial credit for an extra credit assignment for the Biochemistry I class.


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Madhuri Jayathirtha
    • 1
  • Danielle Whitham
    • 1
  • Sydney Stradtman
    • 1
  • Costel C. Darie
    • 1
    Email author
  1. 1.Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular ScienceClarkson UniversityPotsdamUSA

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