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Recent Applications of Mass Spectrometry at Clarkson University

  • Madhuri Jayathirtha
  • Danielle Whitham
  • Sydney Stradtman
  • Costel C. DarieEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1140)

Abstract

Mass spectrometry (MS) is a powerful technique that has various applications including the identification and characterization of proteins, protein-protein interactions and protein post translational modifications, as well as other molecules (i.e. metabolites, lipids, nucleotides and polynucleotides). However, not too many undergraduate students within the USA and around the world have access to (and are trained in) MS. The undergraduate students in our department are taught to analyze proteomics and metabolomics data obtained from MS analysis, including de novo sequencing of peptides and to interpret the MS and MS/MS data acquired in positive and negative ionization modes. Here, we give some examples of MS data analyzed in the Biochemistry I class and then examples of some independent research projects performed by students over the years in the Biochemistry and Biotechnology laboratory, where MS is used for both proteins, peptides and metabolites analysis, thus demonstrating the applicability of MS analysis in diverse fields. The projects discussed include analysis of the protein content present in yogurt, beer, protein shakes, contact lenses, or milk of animal or vegetal origin.

Keywords

Mass spectrometry Proteomics Metabolomics Undergraduate students 

Abbreviations

BCA

Bicinchoninic acid protein assay

ESI

Electron spray ionization

MALDI

Matrix assisted laser desorption ionization

MS

Mass spectrometry

Pkl

Peaklist

PLGS

Protein Lynx Global Server

PMFA

Proteins migrating faster than albumin

QTof

Quadruple time of flight

SDS-PAGE

Sodium dodecyl sulfate-poly acrylamide gel electrophoresis

TIC

Total ion chromatogram

UPLC

Ultra performance liquid chromatography

Notes

Acknowledgement

We would like to thank all the students, who did their independent research projects in Biochemistry and Biotechnology lab course using MS method for analysis and other students in the lab for creating a pleasant environment. Part of this chapter was partial credit for an extra credit assignment for the Biochemistry I class.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Madhuri Jayathirtha
    • 1
  • Danielle Whitham
    • 1
  • Sydney Stradtman
    • 1
  • Costel C. Darie
    • 1
    Email author
  1. 1.Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular ScienceClarkson UniversityPotsdamUSA

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