Analytical Methods for Mass Spectrometry-Based Metabolomics Studies

  • Siyu Wang
  • Ian A. Blair
  • Clementina MesarosEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1140)


The advancement of mass spectrometry-based analytical platform largely facilitates small-molecule metabolomics studies, which allows simultaneously analysis of a large number of metabolites from bio-samples and give a general picture of metabolic changes related to diseases or environmental alteration. Due to the large diversity of cellular metabolites, globally and precisely examining metabolic profile remains the most challenging part in metabolomic experiment. Mass spectrometry coupled with liquid chromatography enhances sensitivity and resolving power of metabolites identification and quantification, as well as versatility of analyzing a wide array of metabolites. In this chapter, we discussed the technical aspects of each step in the workflow of metabolomics studies we aimed to give technical guidelines for metabolomics investigation design and approach.


Metabolomics Liquid chromatography-mass spectrometry HILIC Ion pairing chromatography Polar metabolites Glycolysis Tricarboxylic acids cycle Pentose phosphate pathway 


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Authors and Affiliations

  1. 1.Penn SRP Center and Center of Excellence in Environmental Toxicology Center, Department of Systems Pharmacology and Translational Therapeutics, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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